Serotypes and Antimicrobial Susceptibility of Actinobacillus pleuropneumoniae Isolated from Piglets with Pleuropneumonia.

Asawa, Tamae; Kobayashi, Hideki; Mitani, Kenji; Ito, Nobuyoshi; Morozumi, Tatsuya

doi:10.1292/jvms.57.757

Cited by 26 publications

(41 citation statements)

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“…There was a high resistance rate to particular antimicrobials, notably TC, FF, ABPC, PCG, and TMP/SMX in this study. Although previous reports revealed a relatively high degree of activity against A. pleuropneumoniae isolates (Asawa et al 1995), TC and TMP/SMX are still among the recommended antimicrobials for PP therapy . Nevertheless, since these antimicrobials have been used extensively for the treatment of numerous swine diseases over the past several decades, increasing resistance rates have been reported in several European countries (Guti errez-Mart ın et al 2006).…”

Section: Discussionmentioning

confidence: 95%

“…Some studies have reported that there is no correlation between A. pleuropneumoniae serotype and antimicrobial resistance (Matter et al 2007;Kucerova et al 2011), whereas other studies concluded that different A. pleuropneumoniae serotypes displayed varying levels of antimicrobial resistance (Asawa et al 1995;Lee et al 2015). Such heterogeneous antimicrobial resistance among serotypes has caused significant problems for controlling PP.…”

Section: Discussionmentioning

confidence: 99%

“…Antibiotic selective pressure is known to affect resistance in commensal Actinobacillus strains that reside in the tonsils without causing disease (Matter et al 2007). Previous studies have reported that different A. pleuropneumoniae serotypes displayed varying levels of antimicrobial resistance (Asawa et al 1995;Lee et al 2015). Such heterogeneous antimicrobial resistance among the different serotypes has caused significant problems for PP treatment.…”

Section: Introductionmentioning

confidence: 99%

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Molecular serotyping and antimicrobial resistance profiles ofActinobacillus pleuropneumoniaeisolated from pigs in South Korea

Kim

Hur

Lee

et al. 2016

Veterinary Quarterly

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Background: Actinobacillus pleuropneumoniae (APP) causes porcine pleuropneumonia (PP). Objective: Serotypes and antimicrobial resistance patterns in APP isolates from pigs in Korea were examined. Methods: Sixty-five APP isolates were genetically serotyped using standard and multiplex PCR (polymerase chain reaction). Antimicrobial susceptibilities were tested using the standardized disk-agar method. PCR was used to detect b-lactam, gentamicin and tetracycline-resistance genes. The random amplified polymorphic DNA (RAPD) patterns were determined by PCR. Results: Korean pigs predominantly carried APP serotypes 1 and 5. Among 65 isolates, one isolate was sensitive to all 12 antimicrobials tested in this study. Sixty-two isolates was resistant to tetracycline and 53 isolates carried one or five genes including tet(B), tet(A), tet(H), tet(M)/tet (O), tet(C), tet(G) and/or tet(L)-1 markers. Among 64 strains, 9% and 26.6% were resistance to 10 and three or more antimicrobials, respectively. Thirteen different antimicrobial resistance patterns were observed and RAPD analysis revealed a separation of the isolates into two clusters: cluster II (6 strains resistant to 10 antimicrobials) and cluster I (the other 59 strains). Conclusion: Results show that APP serotypes 1 and 5 are the most common in Korea, and multidrug resistant strains are prevalent. RAPD analysis demonstrated that six isolates resistant to 10 antimicrobials belonged to the same cluster.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular serotyping and antimicrobial resistance profiles ofActinobacillus pleuropneumoniaeisolated from pigs in South Korea

Kim

Hur

Lee

et al. 2016

Veterinary Quarterly

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“…Serovars 1 and 2 are common in Japan [1]. Serovar 2 is predominant in Korea [11], in Spain, serovars 2 and 4 are predominant [8].…”

Section: Discussionmentioning

confidence: 98%

“…The distributions of serovars differ among countries [1,8,11]. Serovars 1, 2, 3, 5, 7, and 8 of A. pleuropneumoniae have been isolated and confirmed in Taiwan [3,6,9,10]; serovar 1 was the predominate isolate [5].…”

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confidence: 99%

Serotypes, Antimicrobial Susceptibility, and Minimal Inhibitory Concentrations of Actionbacillus pleuropneumoniae Isolated from Slaughter Pigs in Taiwan (2002-2007)

Yang

Lin

et al. 2011

J. Vet. Med. Sci.

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ABSTRACT. In total, 211 isolates of A. pleuropneumoniae were collected from pigs with hemorrhagic pneumonia at slaughterhouses during [2002][2003][2004][2005][2006][2007]. Serotypes, antimicrobial susceptibility and minimum inhibitory concentration (MIC) values were determined for each isolate of A. pleuropneumoniae to 10 antimicrobial agents. Serovar 1 of A. pleuropneumoniae was predominant in Taiwan in 138 of the 211 isolates, followed by serovars 2 and 5. More than 90% of collected isolates were sensitive to ceftiofur, cephalothin, and chloramphenical. However, lincospectin and gentamicin were relatively less susceptible with sensitivities of only 2.4 and 5.7%, respectively. Additionally, ceftiofur had the highest in vitro activity with an MIC 50 of 2.2 µg/ml, followed by cephalothin (2.7 µg/ml) and chloramphenicol (7.9 µg/ ml). Lincospectin had the least activity with MIC 50 and MIC 90 values of 73.9 and 114.5 µg/ml, respectively. The data indicate that ceftiofur and cephalothin were extremely active against A. pleuropneumoniae and with minimum MIC values. These drugs are suitable for controlling and treating hemorrhagic pleuropneumonia outbreaks in swine.KEY WORDS: Actinobacillus pleuropneumoniae, antimicrobial susceptibility, minimum inhibitory concentration, serotype, swine.J. Vet. Med. Sci. 73(2): 205-208, 2011 Porcine necrotizing hemorrhagic pleuropneumonia caused by Actinobacillus pleuropneumoniae is one of the most costly respiratory diseases in swine industries. The acute type of this disease is highly contagious and has a high mortality rate, it is economically important for pig producers [15].Fifteen serovars of A. pleuropneumoniae have been recognized [2]. The distributions of serovars differ among countries [1,8,11]. Serovars 1, 2, 3, 5, 7, and 8 of A. pleuropneumoniae have been isolated and confirmed in Taiwan [3,6,9,10]; serovar 1 was the predominate isolate [5].Administering antimicrobial agents is the most common and effective strategy employed for controlling and treating this lethal disease in the field. Several antimicrobial agents have been employed clinically. Since antimicrobial susceptibility can change over time in the same area [8], an effective treatment using antimicrobial agents against A. pleuropneumoniae in the field requires detailed information about the antimicrobial activities of antimicrobial agents. This study investigates the serotypes of A. pleuropneumoniae isolates and in vitro activity of antimicrobial agents against A. pleuropneumoniae isolates collected from slaughterhouse pigs in Taiwan. MATERIALS AND METHODSA. pleuropneumoniae isolation and serotyping: In total, 685 lung specimens with typical hemorrhagic necrotizing pneumonia obtained from 5 pig slaughterhouses were collected during [2002][2003][2004][2005][2006][2007]. All isolates were cultured on tryptic soy blood agar plates (Difco Lab., Detroit, MI, U.S.A.) with 1% nicotinamide adenine dinucleotide (SigmaAldrich, St. Louis, MO, U.S.A.). Plates were incubated for 24 hr at 37°C under 5% CO 2 . Isolates were ...

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Relationship between serotype and the antimicrobial susceptibility of Mannheimia haemolytica isolates collected between 1991 and 2010

Katsuda

Kohmoto

Mikami

2013

Research in Veterinary Science

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Serotypes and Antimicrobial Susceptibility of Actinobacillus pleuropneumoniae Isolated from Piglets with Pleuropneumonia.

Cited by 26 publications

References 0 publications

Molecular serotyping and antimicrobial resistance profiles ofActinobacillus pleuropneumoniaeisolated from pigs in South Korea

Molecular serotyping and antimicrobial resistance profiles ofActinobacillus pleuropneumoniaeisolated from pigs in South Korea

Serotypes, Antimicrobial Susceptibility, and Minimal Inhibitory Concentrations of Actionbacillus pleuropneumoniae Isolated from Slaughter Pigs in Taiwan (2002-2007)

Relationship between serotype and the antimicrobial susceptibility of Mannheimia haemolytica isolates collected between 1991 and 2010

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