Serotypes and anti-microbial susceptibility of Plesiomonas shigelloides isolates from humans, animals and aquatic environments in different countries

González-Rey, Carlos; Svenson, Stefan B.; Bravo, Laura; Siitonen, Anja; Pasquale, Vincenzo; Dumontet, Stefano; Ciznár, I; Krovacek, Karel

doi:10.1016/j.cimid.2003.08.001

Cited by 32 publications

(27 citation statements)

References 24 publications

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“…The two genera of bacteria expressed varying levels of resistance to erythromycin. Such high susceptibility levels of fish bacteria have been reported elsewhere (Aravena-Román et al 2012;DePaola et al 1995;Gonzalez-Rey et al 2004). In a study by Akinbowale and others (Akinbowale et al 2006), the Plesiomonas isolate tested was sensitive to all drugs tested except kanamycin, however, their study observed varying levels of resistance by other bacteria to several antibiotics.…”

Section: Discussionmentioning

confidence: 67%

Occurrence and antibiotic susceptibility of fish bacteria isolated from Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish) in Uganda

et al. 2018

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The intention of this study was to identify the bacterial pathogens infecting Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish), and to establish the antibiotic susceptibility of fish bacteria in Uganda. A total of 288 fish samples from 40 fish farms (ponds, cages, and tanks) and 8 wild water sites were aseptically collected and bacteria isolated from the head kidney, liver, brain and spleen. The isolates were identified by their morphological characteristics, conventional biochemical tests and Analytical Profile Index test kits. Antibiotic susceptibility of selected bacteria was determined by the Kirby-Bauer disc diffusion method. The following well-known fish pathogens were identified at a farm prevalence of; Aeromonas hydrophila (43.8%), Aeromonas sobria (20.8%), Edwardsiella tarda (8.3%), Flavobacterium spp. (4.2%) and Streptococcus spp. (6.3%). Other bacteria with varying significance as fish pathogens were also identified including Plesiomonas shigelloides (25.0%), Chryseobacterium indoligenes (12.5%), Pseudomonas fluorescens (10.4%), Pseudomonas aeruginosa (4.2%), Pseudomonas stutzeri (2.1%), Vibrio cholerae (10.4%), Proteus spp. (6.3%), Citrobacter spp. (4.2%), Klebsiella spp. (4.2%) Serratia marcescens (4.2%), Burkholderia cepacia (2.1%), Comamonas testosteroni (8.3%) and Ralstonia picketti (2.1%). Aeromonas spp., Edwardsiella tarda and Streptococcus spp. were commonly isolated from diseased fish. Aeromonas spp. (n = 82) and Plesiomonas shigelloides (n = 73) were evaluated for antibiotic susceptibility. All isolates tested were susceptible to at-least ten (10) of the fourteen antibiotics evaluated. High levels of resistance were however expressed by all isolates to penicillin, oxacillin and ampicillin. This observed resistance is most probably intrinsic to those bacteria, suggesting minimal levels of acquired antibiotic resistance in fish bacteria from the study area. To our knowledge, this is the first study to establish the occurrence of several bacteria species infecting fish; and to determine antibiotic susceptibility of fish bacteria in Uganda. The current study provides baseline information for future reference and fish disease management in the country.

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Section: Discussionmentioning

confidence: 67%

Occurrence and antibiotic susceptibility of fish bacteria isolated from Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish) in Uganda

et al. 2018

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“…P. shigelloides, A. hydrophila A. baumannii, and P. fluorescens are opportunistic in nature and may cause secondary infections in immunocompromised marine mammals and humans. Therefore, the resistance patterns that they exhibit are of particular interest (Palleroni, 1984;Mathewson and Dupont, 1992;Gonzalez-Rey et al, 2004). P. shigelloides is most commonly resistant to piperacillin and intermediate in resistance to erythromycin, whereas ciprofloxacin and tetracycline are typically effective antibiotic treatment in humans (Reinhardt and George, 1985;Stock and Wiedmann, 2001).…”

Section: Discussionmentioning

confidence: 98%

Antibiotic-Resistant Organisms Cultured from Atlantic Bottlenose Dolphins (Tursiops truncatus) Inhabiting Estuarine Waters of Charleston, SC and Indian River Lagoon, FL

et al. 2009

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Bottlenose dolphins (Tursiops truncatus) from estuarine waters of Indian River Lagoon, FL (IRL) and Charleston, SC (CHS) were cultured to screen for microorganism colonization and to assess antibiotic sensitivity. Swabs (n = 909) were collected from the blowhole, gastric fluid, and feces of 171 individual dolphins The most frequently cultured organisms were Plesiomonas shigelloides (n = 161), Aeromonas hydrophila (n = 144), Escherichia coli (n = 85), and Pseudomonas fluorescens (n = 82). In descending frequency, organisms demonstrated resistance to erythromycin, ampicillin, and cephalothin. Human and animal pathogens resistant to antibiotics used in human and veterinary medicine were cultured. Escherichia coli (E. coli) more often was resistant in IRL dolphins. Three cases of methicillin-resistant Staphylococcus aureus (MRSA) were found at CHS. Emergence of antibiotic resistance is not confined to humans. Bottlenose dolphins may serve as sentinels for transfer of resistance from humans and animals or indicate that antibiotics are reaching the marine environment and causing resistance to emerge through selective pressure and genetic adaptation.

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“…This scheme describes about 100 O types and 50 H types, with a high number of combinations of these types. Correlation between some serovars and sources has been suggested (27).…”

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confidence: 99%

Recombining Population Structure of Plesiomonas shigelloides ( Enterobacteriaceae ) Revealed by Multilocus Sequence Typing

et al. 2007

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Plesiomonas shigelloides is an emerging pathogen that is widespread in the aquatic environment and is responsible for intestinal diseases and extraintestinal infections in humans and other animals. Virtually nothing is known about its genetic diversity, population structure, and evolution, which severely limits epidemiological control. We addressed these questions by developing a multilocus sequence typing (MLST) system based on five genes (fusA, leuS, pyrG, recG, and rpoB) and analyzing 77 epidemiologically unrelated strains from several countries and several ecological sources. The phylogenetic position of P. shigelloides within family Enterobacteriaceae was precisely defined by phylogenetic analysis of the same gene portions in other family members. Within P. shigelloides, high levels of nucleotide diversity (average percentage of nucleotide differences between strains, 1.49%) and genotypic diversity (64 distinct sequence types; Simpson's index, 99.7%) were found, with no salient internal phylogenetic structure. We estimated that homologous recombination in housekeeping genes affects P. shigelloides alleles and nucleotides 7 and 77 times more frequently than mutation, respectively. These ratios are similar to those observed in the naturally transformable species Streptococcus pneumoniae with a high rate of recombination. In contrast, recombination within Salmonella enterica, Escherichia coli, and Yersinia enterocolitica was much less frequent. P. shigelloides thus stands out among members of the Enterobacteriaceae. Its high rate of recombination results in a lack of association between genomic background and O and H antigenic factors, as observed for the 51 serotypes found in our sample. Given its robustness and discriminatory power, we recommend MLST as a reference method for population biology studies and epidemiological tracking of P. shigelloides strains.Plesiomonas shigelloides is a species of rod-shaped gramnegative bacteria recently classified in the family Enterobacteriaceae and is the only oxidase-positive member of this family (32). Freshwater and estuarine water are considered to be the natural environment of P. shigelloides (36), which is often isolated from fish and seafood (30,42). Like many Enterobacteriaceae species, P. shigelloides is also found in a wide range of hosts, including dogs,

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Serotypes and anti-microbial susceptibility of Plesiomonas shigelloides isolates from humans, animals and aquatic environments in different countries

Cited by 32 publications

References 24 publications

Occurrence and antibiotic susceptibility of fish bacteria isolated from Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish) in Uganda

Occurrence and antibiotic susceptibility of fish bacteria isolated from Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish) in Uganda

Antibiotic-Resistant Organisms Cultured from Atlantic Bottlenose Dolphins (Tursiops truncatus) Inhabiting Estuarine Waters of Charleston, SC and Indian River Lagoon, FL

Recombining Population Structure of Plesiomonas shigelloides ( Enterobacteriaceae ) Revealed by Multilocus Sequence Typing

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