Serotonin uptake and imipramine binding in blood platelets and brain of Fawn-hooded and Sprague Dawley rats

The Flinders Sensitive Line Rat Model of Depression—25 Years and Still Producing

“…A similar finding has been reported in humans (Pettinati, 2004). The FH rats exhibit abnormalities in central serotonergic function (Arora et al, 1983;Aulakh et al, 1994;Bendotti and Samanin, 1987;DumbrilleRoss and Tang, 1981), but whether these serotonergic abnormalities contribute to their behavior remains to be determined. Interestingly, the first results showing decreased NPY expression in hippocampal fibers in a model of depression were obtained in FH rats; these changes were reversed following electroconvulsive stimuli (ECS) (Mathé et al, 1998).…”

Sex-Dependent Modulation of Ethanol Consumption in Vesicular Monoamine Transporter 2 (VMAT2) and Dopamine Transporter (DAT) Knockout Mice

“…Reduced cerebrospinal fluid levels of the serotonin metabolite 5-HIAA are observed in some alcoholics (Linnoila et al, 1983), and are associated with increased ethanol consumption in rhesus monkeys (Higley et al, 1996). Ethanol-preferring rats display alterations in tissue serotonin levels (McBride et al, 1990Aulakh et al, 1994;Zhou et al, 1994), responses to serotoninergic agents (Gudelsky et al, 1985;Aulakh et al, 1988aAulakh et al, , b, 1992Aulakh et al, , 1994Wang et al, 1988), serotonin reuptake (Arora et al, 1983;Hulihan-Giblin et al, 1993;Chen and Lawrence, 2000), and serotonin receptor densities (Hulihan-Giblin et al, 1992Wong et al, 1993;McBride et al, 1994McBride et al, , 1997Chen and Lawrence, 2000). A variety of serotonergic agents can reduce voluntary ethanol consumption in animal models and in humans (for a review, see Lovinger, 1999;Myrick et al, 2001), and serotonin receptor gene KOs also affect ethanol self-administration (Crabbe et al, 1996;Risinger et al, 1996Risinger et al, , 1999Risinger et al, , 2000Rubinstein et al, 1997;El-Ghundi et al, 1998;Phillips et al, 1998).…”

Differential Neuroendocrine Responses to the 5-HT Agonist m-Chlorophenylpiperazine in Fawn-Hooded Rats Relative to Wistar and Sprague-Dawley Rats

“…Blood platelets from FH rats are deficient in storage, release, and uptake of serotonin (5-HT) [2,30], Platelets have been used as a model for the study of cen tral serotoninergic nerve function because of the similari ties in the manner in which both store and metabolize 5-HT. Binding experiments with [!H]-imipramine ('H-IMI) in pla telets and brain tissue suggest that the 'H-lMI-binding site in both tissues is identical and may be related to the 5-HTuptake site [4], Some reports have suggested either an ab sence [8] or a diminished number [2] of 'H-IMI-binding sites on platelets and in brain tissue of FH rats, although these findings have been disputed [13,23].…”

“…Blood platelets from FH rats are deficient in storage, release, and uptake of serotonin (5-HT) [2,30], Platelets have been used as a model for the study of cen tral serotoninergic nerve function because of the similari ties in the manner in which both store and metabolize 5-HT. Binding experiments with [!H]-imipramine ('H-IMI) in pla telets and brain tissue suggest that the 'H-lMI-binding site in both tissues is identical and may be related to the 5-HTuptake site [4], Some reports have suggested either an ab sence [8] or a diminished number [2] of 'H-IMI-binding sites on platelets and in brain tissue of FH rats, although these findings have been disputed [13,23]. Although brain 5-HT concentrations were not found to be diminished in the brains of FH rats relative to Sprague-Dawley (SD) rats [15], several studies have examined the possibility that FH rats have an altered central nervous system serotoninergic function [II,34], w-Chlorophenylpiperazine (MCPP), a metabolite of the antidepressant trazodone, has been shown to have high affinity for 5-HT receptors in radioligand studies [29].…”