Serotonin antagonists induce anxiolytic and anxiogenic-like behavior in zebrafish in a receptor-subtype dependent manner

Nowicki, Magda; Tran, Steven; Muraleetharan, Arrujyan; Marković, Stefan

doi:10.1016/j.pbb.2014.09.022

Cited by 75 publications

(40 citation statements)

References 60 publications

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“…In both the light/dark and novel tank tests, the 5-HT 1A receptor antagonist WAY 100,635 decreases anxiety-like behaviour (Maximino et al, 2013b); however, the antagonist p-MPPF increases anxiety-like behaviour in the novel tank test (Nowicki et al, 2014). Antagonists at 5-HT 2 and 5-HT 3 receptors were shown to increase anxietylike behaviour in the novel tank test (Nowicki et al, 2014), while 5-HT 1B receptor antagonists decrease it (Maximino et al, 2013b;Nowicki et al, 2014).…”

Section: Monoaminergic Innervation In Teleostsmentioning

confidence: 95%

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

Soares¹,

Maximino²

2018

Preprint

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Animal-focused research has been crucial for scientific advancement however, in this matter, rodents are still taking a starring role. Coming out from merely being supportive of evidence found in rodents, the use of fish models has slowly taken a more central role and expanded its overall contributions in areas such as social sciences, evolution, physiology, and recently in translational medical research. In neurosciences, zebrafish has been widely adopted, contributing to our understanding of the genetic control of brain processes, and the effects of pharmacological manipulations. However, discussion continues regarding the paradox of function versus structure, when fish and mammals are compared, and on the potentially evolutionarily conserved nature of behaviour across fish species. From the behavioural stand point we explorted aversive/stress and social behaviour in selected fish models, and refer to the extensive contributions of stress and monoaminergic systems. We suggest that, in spite of marked neuroanatomical differences between fish and mammals, stress and sociality are conserved at the behavioural and molecular levels. We also suggest that stress and sociality are mediated by monoamines in predictable and non-trivial ways, and that monoamines could “bridge” the relationship between stress and social behaviour. To reconcile the level of divergence with the level of similarity, neuroanatomy, pharmacology, behavioural analysis, and ecology studies conducted in the laboratory and in nature need to add to each other and enhance our understanding of fish behaviour and ultimately how this all may translate to better model systems for translational studies.

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Section: Monoaminergic Innervation In Teleostsmentioning

confidence: 95%

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

Soares¹,

Maximino²

2018

Preprint

Self Cite

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“…Immersion-based drug delivery was used because of its less invasive nature compared to other methods of administration (e.g. injection) and frequent use in adult zebrafish research (Nowicki et al, 2014; Tran et al, 2015b; Levin et al, 2007; Conners et al, 2014; Sackerman et al, 2010). …”

Section: Methodsmentioning

confidence: 99%

“…Video recordings were taken from the front of the testing tanks during the 60 minute exposure. The he frontal view allows better video-detection as the fish appear brighter and more distinguishable from the background, and this method has been shown to allow psychopharmacological analysis and quantification of a number of natural behavioural responses in zebrafish (Nowicki et al, 2014). EthoVision XT 8.0 (Noldus Info Tech, Wageningen, The Netherlands) was used to analyze the behavioural recordings and quantify the total distance travelled (cm) fish swam.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of phosphorylated tyrosine hydroxylase attenuates ethanol-induced hyperactivity in adult zebrafish (Danio rerio)

Nowicki

Tran

Chatterjee

2015

Pharmacology Biochemistry and Behavior

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Zebrafish have been successfully employed in the study of the behavioural and biological effects of ethanol. Like in mammals, low to moderate doses of ethanol induce motor hyperactivity in zebrafish, an effect that has been attributed to the activation of the dopaminergic system. Acute ethanol exposure increases dopamine (DA) in the zebrafish brain, and it has been suggested that tyrosine hydroxylase, the rate-limiting enzyme of DA synthesis, may be activated in response to ethanol via phosphorylation. The current study employed tetrahydropapaveroline (THP), a selective inhibitor of phosphorylated tyrosine hydroxylase, for the first time, in zebrafish. We treated zebrafish with a THP dose that did not alter baseline motor responses to examine whether it can attenuate or abolish the effects of acute exposure to alcohol (ethanol) on motor activity, on levels of DA, and on levels of dopamine’s metabolite 3,4-dihydroxyphenylacetic acid (DOPAC). We found that 60-minute exposure to 1% alcohol induced motor hyperactivity and an increase in brain DA. Both of these effects were attenuated by pre-treatment with THP. However, no differences in DOPAC levels were found among the treatment groups. These findings suggest that tyrosine hydroxylase is activated via phosphorylation to increase DA synthesis during alcohol exposure in zebrafish, and this partially mediates alcohol’s locomotor stimulant effects. Future studies will investigate other potential candidates in the molecular pathway to further decipher the neurobiological mechanism that underlies the stimulatory properties of this popular psychoactive drug.

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“…In both the light/dark and novel tank tests, the 5-HT1A receptor antagonist WAY 100,635 decreases defensive behaviour (Maximino et al, 2013b); however, the antagonist p-MPPF increases anxiety-like behaviour in the novel tank test (Nowicki et al, 2014). Antagonists at 5-HT2 and 5-HT3 receptors were shown to increase defensive behaviour in the novel tank test (Nowicki et al, 2014), while 5-HT1B receptor antagonists decrease it (Maximino et al, 2013b;Nowicki et al, 2014).…”

Section: Serotonin and The Aversive Behaviour Network Of Zebrafishmentioning

confidence: 95%

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

Soares

Maximino

2018

Preprint

Self Cite

View full text Add to dashboard Cite

Animal-focused research has been crucial for scientific advancement however, in this matter, rodents are still taking a starring role. Coming out from merely being supportive of evidence found in rodents, the use of fish models has slowly taken a more central role and expanded its overall contributions in areas such as social sciences, evolution, physiology, and recently in translational medical research. In neurosciences, zebrafish has been widely adopted, contributing to our understanding of the genetic control of brain processes, and the effects of pharmacological manipulations. However, discussion continues regarding the paradox of function versus structure, when fish and mammals are compared, and on the potentially evolutionarily conserved nature of behaviour across fish species. From the behavioural stand point we explored aversive/stress and social behaviour in selected fish models, and refer to the extensive contributions of stress and monoaminergic systems. We suggest that, in spite of marked neuroanatomical differences between fish and mammals, stress and sociality are conserved at the behavioural and molecular levels. We also suggest that stress and sociality are mediated by monoamines in predictable and non-trivial ways, and that monoamines could "bridge" the relationship between stress and social behaviour. To reconcile the level of divergence with the level of similarity, we need neuroanatomical, pharmacological, behavioural, and ecological studies conducted in the laboratory and in nature.These areas need to add to each other to enhance our understanding of fish behaviour and ultimately how this all may translate to better model systems for translational studies.

show abstract

Serotonin antagonists induce anxiolytic and anxiogenic-like behavior in zebrafish in a receptor-subtype dependent manner

Cited by 75 publications

References 60 publications

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

Inhibition of phosphorylated tyrosine hydroxylase attenuates ethanol-induced hyperactivity in adult zebrafish (Danio rerio)

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

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