Serotonin and acetylcholine modulate the sensitivity of early sea urchin embryos to protein kinase C activators

Buznikov, G. A.; Маршак, Т. Л.; Мальченко, Л. А.; Nikitina, L. A.; Shmukler, Yuri B.; Buznikov, Alex G.; Rakić, Lj.; Whitaker, Michael

doi:10.1016/s1095-6433(98)10045-4

Cited by 7 publications

(10 citation statements)

References 27 publications

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“…5-HT and ACh antagonists affect cleavage divisions in echinoderms, polychaetes, nudibranchs, amphibians and mammals, most probably, via intracellular receptors . On the other hand, some processes, including cleavage furrow completion and direct blastomere interaction, are probably mediated by 5-HT receptors, localised at the membrane surface (Shmukler, 1993;Buznikov et al, 1998;Shmukler et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Serotonergic-induced ion currents in cleaving sea urchin embryos

Shmukler

Tosti²

2002

Invertebrate Reproduction & Development

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Using the whole-cell voltage clamp technique on early sea urchin embryos at different stages of the cell cycle, we showed that agonists of 5-HT receptor subtypes induce inward currents. The current induced by 5-HT3 receptor agonists is dose dependent and reverses between -25 and + 15 mV suggesting, it is carried by nonspecific ion channels. 5-HT3 receptor agonists showed more pronounced effects when administered during cleavage h o w formation. The effects are accompanied by the increase of steady-state conductance and calcium current activity. These results suggest that serotonergics and ion channel activity play a role in the regulation of cellular events during cleavage division in the early sea urchin embryo, supporting the idea of a protosynapse between blastomeres.

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Section: Introductionmentioning

confidence: 99%

Serotonergic-induced ion currents in cleaving sea urchin embryos

Shmukler

Tosti²

2002

Invertebrate Reproduction & Development

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“…Much research has led to the suggestion that early embryos, at cleavage and gastrulation stages, use 5‐HT, together with GABA and acetylcholine, to regulate cell division and morphogenetic cell movements (Renaud et al 1983; Buznikov 1984). A functional coupling of 5‐HT with a second messenger system through the regulation of protein kinase C (PKC) and a calcium mechanism has been found during cleavage of sea urchin eggs (Buznikov et al 1998; Shmukler et al 1999). 5‐Hydroxytryptamine and 5‐HT antagonists administrated to chick embryos affect early morphogenesis, interfering with primitive streak formation, neurulation and somitogenesis (Palèn et al 1979).…”

Section: Introductionmentioning

confidence: 99%

Serotonin localization in Phallusia mammillata larvae and effects of 5‐HT antagonists during larval development

Pennati¹,

Groppelli²,

Sotgia³

et al. 2001

Dev Growth Differ

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The neurotransmitter 5-hydroxytryptamine (5-HT, serotonin) plays an important role in a wide range of non-neural processes. Using immunofluorescence with an antiserotonin antibody, 5-HT was localized in the brain and in some neurons of the larval tail of Phallusia mammillata. To test the effect of 5-HT on development, we treated embryos with two different 5-HT receptor subtype antagonists. Treatment at the gastrula stage with 10 microM ondansetron, an antagonist of the 5-HT(3) receptor, induced anterior truncation and a short tail. At 10 microM, ritanserin, a 5-HT(2B) receptor antagonist, induced larval phenotypes characterized by a roundish trunk region with flat papillae. The juveniles developed from these larvae had an abnormal cardiocirculatory system: their heart contractions were ineffective and their blood cells accumulated in the heart cavity. We conclude that an appropriate level of 5-HT is necessary for correct development and morphogenesis. Moreover, a different key role for multiple receptors in modulating the morphogenetic effects of 5-HT is suggested.

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“…they weaken embryostatic effects of serotonin antagonists [72][73][74] and evoke the increase in cAMP levels in embryonic cells [75]. At the same time the ability of transmitter ligands to influence the activity of protein kinase C and intracellular free calcium ion levels was also shown in early sea embryos [65,76,77]. The presence and functional activity of both transmitters and second messengers inside the embryonic cells may seem excessive but only at a superficial glance.…”

Section: Embryonic Transmitters -Functions and Specific Featuresmentioning

confidence: 99%