Serotonergic Modulation of Walking in Drosophila

Howard, Clare E.; Chen, Chin‐Lin; Tabachnik, Tanya; Hormigo, Rick; Ramdya, Pavan; Mann, Richard S.

doi:10.1016/j.cub.2019.10.042

Cited by 47 publications

(33 citation statements)

References 101 publications

(161 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, we found that the P -element insertion in 5-HT2A fully abrogated the male lifespan differences between fixed and choice dietary environments, largely by increasing lifespan when nutrients were presented individually (see Figure 2A ). Mutant flies were modestly short-lived on the standard, fixed diet, which is likely due to the pleotropic effects of this receptor ( Gasque et al, 2013 ; Chakraborty et al, 2019 ; Howard et al, 2019 ). We also investigated the influence of RNAi-based knockdown of 5-HT2A expression using multiple RNAi lines.…”

Section: Resultsmentioning

confidence: 99%

Drosophila serotonin 2A receptor signaling coordinates central metabolic processes to modulate aging in response to nutrient choice

Lyu

Weaver

Shaukat

et al. 2021

eLife

View full text Add to dashboard Cite

It has been recognized for nearly a century that diet modulates aging. Despite early experiments suggesting that reduced caloric intake augmented lifespan, accumulating evidence indicates that other characteristics of the diet may be equally or more influential in modulating aging. We demonstrate that behavior, metabolism, and lifespan in Drosophila are affected by whether flies are provided a choice of different nutrients or a single, complete medium, largely independent of the amount of nutrients that are consumed. Meal choice elicits a rapid metabolic reprogramming that indicates a potentiation of TCA cycle and amino acid metabolism, which requires serotonin 2A receptor. Knockdown of glutamate dehydrogenase, a key TCA pathway component, abrogates the effect of dietary choice on lifespan. Our results reveal a mechanism of aging that applies in natural conditions, including our own, in which organisms continuously perceive and evaluate nutrient availability to promote fitness and well-being.

show abstract

Section: Resultsmentioning

confidence: 99%

Drosophila serotonin 2A receptor signaling coordinates central metabolic processes to modulate aging in response to nutrient choice

Lyu

Weaver

Shaukat

et al. 2021

eLife

View full text Add to dashboard Cite

show abstract

“…Behavioral studies of walking flies demonstrate that vibration of the substrate can cause flies to stop walking ( Fabre et al, 2012 ; Howard et al, 2019 ). To determine if 10Bα neurons could drive this pausing behavior, we optogenetically activated 10Bα neurons in tethered, intact flies walking on a spherical treadmill ( Figure 8A ).…”

Section: Resultsmentioning

confidence: 99%

“…Instead, 10Bα neurons drive pauses in walking behavior. Previous behavioral experiments found that flies will stop walking when they sense the ground vibrating ( Fabre et al, 2012 ; Howard et al, 2019 ) – 10Bα neurons may mediate this stopping via vibration-sensitive inputs from the FeCO. If true, this would imply that the FeCO functions as both a proprioceptive and exteroceptive organ.…”

Section: Discussionmentioning

confidence: 99%

Central processing of leg proprioception in Drosophila

Agrawal

Dickinson

Sustar

et al. 2020

eLife

View full text Add to dashboard Cite

Proprioception, the sense of self-movement and position, is mediated by mechanosensory neurons that detect diverse features of body kinematics. Although proprioceptive feedback is crucial for accurate motor control, little is known about how downstream circuits transform limb sensory information to guide motor output. Here, we investigate neural circuits in Drosophila that process proprioceptive information from the fly leg. We identify three cell-types from distinct developmental lineages that are positioned to receive input from proprioceptor subtypes encoding tibia position, movement, and vibration. 13Bα neurons encode femur-tibia joint angle and mediate postural changes in tibia position. 9Aα neurons also drive changes in leg posture, but encode a combination of directional movement, high frequency vibration, and joint angle. Activating 10Bα neurons, which encode tibia vibration at specific joint angles, elicits pausing in walking flies. Altogether, our results reveal that central circuits integrate information across proprioceptor subtypes to construct complex sensorimotor representations that mediate diverse behaviors, including reflexive control of limb posture and detection of leg vibration.

show abstract

“…It appears that the biogenic amine serotonin (5-HT) is involved in such place learning (Sitaraman et al, 2008(Sitaraman et al, , 2017Sitaraman and LaFerriere, 2020). Furthermore, different 5-HT subsets or 5-HT regulation interfere with feeding (Albin et al, 2015;Liu et al, 2015), food seeking behaviour (He et al, 2020), locomotion (Yellman et al, 1997;Howard et al, 2019), sleep architecture (Liu et al, 2019), and quiescence (Pooryasin and Fiala, 2015). Thus, 5-HT manipulation provides a good candidate for further studies in the Panopticon (Tierney, 2020).…”

Section: Discussionmentioning

confidence: 99%

Long-lasting impact of starvation experience on fly activity and place preference

Mahishi

Triphan

Hesse

et al. 2020

Preprint

View full text Add to dashboard Cite

Animal behaviours are demonstrably governed by sensory stimulation, previous experience and internal states like hunger. With increasing hunger, priorities shift towards foraging and feeding. During foraging, flies are known to employ efficient path integration strategies. However, general long-term activity patterns for both hungry and satiated flies in conditions of foraging remain to be better understood. Similarly, little is known about how chronic contact chemosensory stimulation affects locomotion. To address these questions, we have developed a novel, simplistic fly activity tracking setup – the Panopticon. Using a 3D-printed Petri dish inset, our assay allows recording of walking behaviour, of several flies in parallel, with all arena surfaces covered by a uniform substrate layer. We tested two constellations of providing food: i) in single patches, and ii) omnipresent within the substrate layer. Fly tracking is done with FIJI, further assessment, analysis and presentation is done with a custom-built MATLAB analysis framework. We find that starvation history leads to a long-lasting reduction in locomotion, as well as a delayed place preference for food patches not driven by immediate hunger motivation.

show abstract

Serotonergic Modulation of Walking in Drosophila

Cited by 47 publications

References 101 publications

Drosophila serotonin 2A receptor signaling coordinates central metabolic processes to modulate aging in response to nutrient choice

Drosophila serotonin 2A receptor signaling coordinates central metabolic processes to modulate aging in response to nutrient choice

Central processing of leg proprioception in Drosophila

Long-lasting impact of starvation experience on fly activity and place preference

Contact Info

Product

Resources

About