Serotipos y patrones de resistencia antibiótica en aislados betahemolíticos de Streptococcus agalactiae de madres colonizadas y recién nacidos con enfermedad invasiva

Liébana-Martos, María del Carmen; Cabrera-Alavargonzalez, Jorge; Rodríguez‐Grangér, Javier; Miranda-Casas, Consuelo; Sampedro-Martínez, Antonio; Gutiérrez‐Fernández, José; Rosa-Fraile, Manuel; Navarro-Marí, José María

doi:10.1016/j.eimc.2014.02.023

Cited by 8 publications

(9 citation statements)

References 32 publications

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“…The percentage of NT strains found resembles data reported by Liébana-Martos et al (2015), who detected 6.40% of these isolates [41]. This lack of reactivity with the agglutination test may be due to poor expression of the capsule.…”

Section: Serotype Determinationsupporting

confidence: 87%

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Prevalence, serotypes and virulence genes of Streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation

et al. 2021

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Background In pregnant women Streptococcus agalactiae (GBS) can be transmitted to newborn causing severe infections. It is classified into 10 serotypes (Ia, Ib, II-IX). The severity of neonatal disease is determined by the capsular serotype and virulence factors such as the polysaccharide capsule, encoded by the cps gene, protein C, which includes the Cα surface proteins (bca gene), Rib (rib gene) and Cβ (bac gene); the proteins Lmb (lmb gene), FbsB (fbsB gene), FbsA (fbsA gene), the cyl operon encoding a β-hemolysin (hylB gene), the CAMP factor (cfb gene) and the C5a peptidase (scpB gene). The aim of this work was to determine the degree of GBS colonization in pregnant women, the serotypes distribution and to investigate virulence-associated genes. Methods We worked with 3480 samples of vagino-rectal swabs of women with 35–37 weeks of gestation. The identification of the strains was carried out using conventional biochemical tests and group confirmatory serology using a commercial latex particle agglutination kit. Two hundred GBS strains were selected. Their serotype was determined by agglutination tests. The monoplex PCR technique was used to investigate nine virulence-associated genes (cps, bca, rib, bac, lmb, fbsB, fbsA, hylB and scpB). Results The maternal colonization was 9.09%. The serotypes found were: Ia (33.50%), III (19.00%), Ib (15.50%), II (14.00%), V (7.00%) and IX (5.50%). 5.50% of strains were found to be non-serotypeable (NT). The nine virulence genes investigated were detected simultaneously in 36.50% of the strains. The genes that were most frequently detected were scpB (100.00%), fbsA (100.00%), fbsB (100.00%), cylB (95.00%), lmb (94.00%) and bca (87.50%). We found associations between serotype and genes bac (p = 0.003), cylB (p = 0.02), rib (p = 0.01) and lmb (p < 0.001). Conclusions The frequency of vaginal-rectal colonization, serotypes distribution and associated virulence genes, varies widely among geographical areas. Therefore, epidemiological surveillance is necessary to provide data to guide decision-making and planning of prevention and control strategies.

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Section: Serotype Determinationsupporting

confidence: 87%

“…However, studies conducted by Poyart et al (2007) and Imperi et al (2010) reported molecular methods that erroneously identified some strains [43,44]. Therefore, the use of two different techniques was suggested for special cases of NT strains [41].…”

Section: Serotype Determinationmentioning

confidence: 99%

Prevalence, serotypes and virulence genes of Streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation

et al. 2021

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“…Because of the streptococcus agalactiae infectious leading to early neonatal morbidity and mortality,the streptococcus agalactiae examination is important for pregnant women. Many meta-analysis studies shown that approximately 50% streptococcus agalactiae colonization pregnant women maybe transmit the bacteria by delivery process to their newborns now [27][28][29].The populations in developing or underdeveloped countries have different lifestyles, live in different geographical locations, different ages, socioeconomic levels, schooling, and gestational ages and have different importance for streptococcus agalactiae infectious [11,12,15,18,[30][31][32][33][34]. it is essential to improve and extend streptococcus agalactiae screening performance and accuracy in pregnant women, especially underdeveloped countries.…”

Section: Discussionmentioning

confidence: 99%

To Evaluate Impact on Detection Rate of Streptococcus Agalactiae in the Third Trimester of Pregnancy

Jiang

Jia

et al. 2023

Preprint

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Background: The streptococcus agalactiae infectious leading to early neonatal morbidity and mortality,the streptococcus agalactiae screen became an important examination for pregnant women. The Centers for Disease Control and Prevention (CDC) recommends whole detection protocols of isolation and culture on streptococcus agalactiae.However, The essential factors including incubation time of agar plates, enrichment time of broth, and transport media storage conditions be ignored. This study was designed to understand above easy ignored point and to provide scientific proof for optimization detection method, revision of health standards and prevention and control of disease. Methods: The transport medium without and with enrichment were directly inoculated onto Sheep blood agar plate for 24~48 hours. The positive detection rate of streptococcus agalactiae on different incubation time, with and without enrichment were compared,respectively. The transport medium with enrichment were inoculated for 24~48 hours.The positive detection rate of streptococcus agalactiae on different enrichment time were compared. The transport medium were respectively stored at 2℃~8℃ and 20℃~25℃. Interval 24 hours within 5 days, The recovery rate of streptococcus agalactiae on transport medium different storage temptature were compared. The growth of streptococcus agalactiae colonies were examined for pale pink to red, round and pearly colonies. The isolates colonies were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry to confirm negative or positive. Results: The overall results shown that the detection rate were significantly higher at 48 hours with directly culture methods (P＜0.01)or after enrichment culture methods (P＜0.05). The detection rate have no difference after enrichment for 24 hours or 48 hours (P＞0.05). The recovery rate of streptococcus agalactiae transport medium (stored at 2℃~8℃) were 100% within 4 days and 83.33% at 5th day. However, the recovery rate of streptococcus agalactiae transport medium (stored at 20℃~25℃) were 100% within 2 days,97.22% at 3th day, 52.78% at 4th day and 33.33% at 5th day, respectively. Conclusions: In conclusion, the results suggested that the enrichment culture time was 24 hours, the transport media stable best time is 2 days stored at 20℃~25℃ or 4 days stored at 2℃~8℃, and the best incubation time is 48 hours for streptococcus agalactiae clinical testing in pregnant women.

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“…Six studies reported that colonized pregnant women were referred for prophylaxis, 2–4,6,14,16 and the others presented screening information, but without an approach about prophylactic measures 18–20,26,29,30,41,42,49,50 . Among the pregnant women screened at delivery, only some were treated with antibiotics, whereas there was no report of any type of intervention in this regard in other studies 1,9,21,25,27,43,47 . Two studies dedicated to the study of GBS colonization in other gestational periods reported treatment through the use of antibiotics of pregnant women 15,24 .…”

Section: Discussionmentioning

confidence: 99%

Distribution and Prevalence of Serotypes of Group B Streptococcus Isolated from Pregnant Women in 30 Countries: A Systematic Review

Silva

Oliveira

et al. 2023

Maternal Fetal Med

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Objective: This review aimed to compile scientific data on the distribution and prevalence of group B Streptococcus (GBS) serotypes isolated from pregnant women across 30 countries from 2010 to 2019.Methods: This was a systematic review that addresses the distribution and prevalence of GBS in pregnant women. The search included studies published between January 2010 and December 2019 in PubMed, Virtual Health Library (BVS), ScienceDirect, Scientific Electronic Library Online (SciELO), and LILACS databases. We also surveyed relevant articles published in English, Spanish, and Portuguese between February and April 2020. Original articles, communication, short report, theses, and dissertations were included. The prevalence of GBS colonization, method for capsular serotyping, antimicrobial resistance, distribution and prevalence of serotypes were extracted from each study.Results: In all, 795 publications were identified. After applying the eligibility criteria, 48 articles were included for the final systematic analysis; most articles were from Asia and were published during the years 2014 to 2017. For the identification of serotypes, most studies used the polymerase chain reaction technique. There were records of all 10 GBS serotypes, namely, Ia, Ib, and II-IX, among the countries analyzed. GBS susceptibility and resistance to antibiotics were addressed in 37.5% of the publications analysed. Conclusion:This review showed that GBS serotypes are distributed differently in the 30 analyzed countries, with serotypes Ia, Ib, and II to V being the most prevalent. Furthermore, our results highlighted the relationship of GBS with maternal colonization, implications for neonates, and antibiotic resistance.

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Serotipos y patrones de resistencia antibiótica en aislados betahemolíticos de Streptococcus agalactiae de madres colonizadas y recién nacidos con enfermedad invasiva

Cited by 8 publications

References 32 publications

Prevalence, serotypes and virulence genes of Streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation

Prevalence, serotypes and virulence genes of Streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation

To Evaluate Impact on Detection Rate of Streptococcus Agalactiae in the Third Trimester of Pregnancy

Distribution and Prevalence of Serotypes of Group B Streptococcus Isolated from Pregnant Women in 30 Countries: A Systematic Review

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