Seroreactive species-specific lipooligosaccharides of Mycobacterium mucogenicum sp. nov. (formerly Mycobacterium chelonae-like organisms): identification and chemical characterization

Muñoz, Manuel; Raynaud, Catherine; Lanéelle, Marie‐Antoinette; Julián, Esther; López-Marı́n, Luz M.; Silve, Gaby; Ausina, Vicente; Daffé, Mamadou; Luquin, Marina

doi:10.1099/00221287-144-1-137

Cited by 19 publications

(17 citation statements)

References 39 publications

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“…T type II isolates exhibited only 0.6% partial rpoB gene sequence divergence with the region described by Kim et al (30). However, only one isolate has been described as M. mucogenicum ATCC 49650 T , whereas a large collection of M. mucogenicum isolates was more closely related to M. mucogenicum ATCC 49651 by cell wall analysis (45); likewise, our data confirm that the vast majority of M. mucogenicum isolates are closely related to the latter strain, suggesting that M. mucogenicum ATCC 49651 could become the type strain for this species. In our study, isolate D13, recovered from an bronchial aspirate, exhibited 1.7% divergence with M. septicum and was the second clinical strain of this emerging species (26,53).…”

Section: Discussionmentioning

confidence: 86%

rpoB -Based Identification of Nonpigmented and Late-Pigmenting Rapidly Growing Mycobacteria

2003

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Nonpigmented and late-pigmenting rapidly growing mycobacteria (RGM) are increasingly isolated in clinical microbiology laboratories. Their accurate identification remains problematic because classification is labor intensive work and because new taxa are not often incorporated into classification databases. Also, 16S rRNA gene sequence analysis underestimates RGM diversity and does not distinguish between all taxa. We determined the complete nucleotide sequence of the rpoB gene, which encodes the bacterial ␤ subunit of the RNA polymerase, for 20 RGM type strains. After using in-house software which analyzes and graphically represents variability stretches of 60 bp along the nucleotide sequence, our analysis focused on a 723-bp variable region exhibiting 83.9 to 97% interspecies similarity and 0 to 1.7% intraspecific divergence. Primer pair Myco-F-Myco-R was designed as a tool for both PCR amplification and sequencing of this region for molecular identification of RGM. This tool was used for identification of 63 RGM clinical isolates previously identified at the species level on the basis of phenotypic characteristics and by 16S rRNA gene sequence analysis. Of 63 clinical isolates, 59 (94%) exhibited <2% partial rpoB gene sequence divergence from 1 of 20 species under study and were regarded as correctly identified at the species level. Mycobacterium abscessus and Mycobacterium mucogenicum isolates were clearly distinguished from Mycobacterium chelonae; Mycobacterium mageritense isolates were clearly distinguished from "Mycobacterium houstonense." Four isolates were not identified at the species level because they exhibited >3% partial rpoB gene sequence divergence from the corresponding type strain; they belonged to three taxa related to M. mucogenicum, Mycobacterium smegmatis, and Mycobacterium porcinum. For M. abscessus and M. mucogenicum, this partial sequence yielded a high genetic heterogeneity within the clinical isolates. We conclude that molecular identification by analysis of the 723-bp rpoB sequence is a rapid and accurate tool for identification of RGM.Rapidly growing mycobacteria (RGM) that require less than 7 days to produce easily visible colonies on solid media (42) comprise 56 environmental species. Fifteen species commonly encountered in humans and animals (7, 39) belong primarily to the Mycobacterium chelonae-abscessus, Mycobacterium fortuitum, and Mycobacterium smegmatis groups (7). They are increasingly encountered in clinical microbiology laboratories (54,65,67,70,76). They are responsible for pseudo-outbreaks of health care-associated septicemia and lung disease following bronchoscopy (1, 2, 37, 69). They are also responsible for colonization of airways (8, 15); skin and soft-tissue infections characterized by slowly progressive granulomatous inflammation, lymphadenitis, disseminated infection, and chronic pulmonary disease (4,7,26,28,66,69,75). Also, communityacquired outbreaks of skin infection following liposuction (43), M. fortuitum furunculosis following footbaths (73), hypersensitivit...

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Section: Discussionmentioning

confidence: 86%

rpoB -Based Identification of Nonpigmented and Late-Pigmenting Rapidly Growing Mycobacteria

2003

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“…In most cases in which the structural basis of colonial variations was investigated, it was found that rough morphotypes were devoid of some free glycolipids that were present in the smooth morphotypes. For example, it has been reported that in Mycobacterium kansasii and Mycobacterium mucogenicum the smooth and rough colony morphologies differ in the presence of trehalose-containing lipooligosaccharides, which are only present in smooth colony surfaces (Belisle and Brennan 1989;Mun˜oz et al 1998). Most Mycobacterium avium complex isolates segregate into smooth-transparent-, smooth-opaque-and rough-colony variants (Moehring and Solotorovsky 1965;Vestal and Kubica 1966;Schaefer et al 1970;Reddy et al 1996).…”

Section: Discussionmentioning

confidence: 99%

“…The NCTC strain is a stable rough variant of the original smooth strain isolated by Stanford and Paul (Stanford and Paul 1973;Stanford et al 2004). Since it has been reported that in the genus Mycobacterium differences in colonial morphologies are directly related to different contents of surface exposed antigens (Barrow and Brennan 1982;Belisle and Brennan 1989;Mun˜oz et al 1998), we believed that it would be interesting to study whether the immunomodulatory properties of smooth and rough colony variants of M. vaccae were the same or presented differences. Therefore, in the present work we have studied the growth conditions in which smooth M. vaccae ATCC 15483 T switches to a rough phenotype; the biochemical and microscopic surface differences between both variants, and the immunological response elicited by mice immunised with smooth or with rough morphotypes.…”

Section: Introductionmentioning

confidence: 98%

The production of a new extracellular putative long-chain saturated polyester by smooth variants of Mycobacterium vaccae interferes with Th1-cytokine production

Rodríguez-Güell

Agustí

Corominas

et al. 2006

Antonie Van Leeuwenhoek

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Mycobacterium vaccae is of major pharmaceutical interest as an immunotherapeutic agent. Although M. vaccae 15483 ATCC(T) strain displays smooth and rough colonial morphologies on solid culture media, it is not known in which conditions M. vaccae switches from one colonial morphotype to the other or whether there are biological differences, especially immunological, between them. We have found that the change from a smooth to rough stable variant occurs spontaneously at 30 degrees C. The analysis of the composition of the cell wall in both variants showed that the smooth morphotype presents an extracellular material that has never previously been described and was identified as a long-chain saturated polyester that, interestingly, is not produced by the rough morphotype. Our results also indicate that this compound could be implicated in the spreading ability of smooth colonies. Proliferation, IFN-gamma and IL-12(p40) production by splenocyte cultures was significantly higher in mice immunised with the rough variant compared with those immunised with the smooth one. This latter finding suggests that the different colonial morphology of M. vaccae may affect the immunomodulatory effects induced from M. vaccae preparations.

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“…LOSs were found and described in Mycobacterium kansasii (6 -8), Mycobacterium gastri (8,9), Mycobacterium szulgai (10), Mycobacterium malmoense (11), Mycobacterium gordonae (12), Mycobacterium butyricum (13), Mycobacterium mucogenicum (14), the Canetti variant of Mycobacterium tuberculosis (15) and, more recently in Mycobacterium marinum (Mma) (16). However, they remain among the less studied mycobacterial glycolipids at a biosynthetic, structural, and functional point of view.…”

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confidence: 99%

Mycobacterium marinum Lipooligosaccharides Are Unique Caryophyllose-containing Cell Wall Glycolipids That Inhibit Tumor Necrosis Factor-α Secretion in Macrophages

Rombouts

Burguière

Maës

et al. 2009

Journal of Biological Chemistry

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Earlier studies have reported a role for lipooligosaccharides (LOSs) in sliding motility, biofilm formation, and infection of host macrophages in Mycobacterium marinum. Although a LOS biosynthetic gene cluster has recently been identified in this species, many structural features of the different LOSs (LOS-I-IV) are still unknown. This clearly hampers assessing the contribution of each LOS in mycobacterial virulence as well as structure-function-based studies of these important cell wallassociated glycolipids. In this study, we have identified an M. marinum isolate, M. marinum 7 (Mma7), which failed to produce LOS-IV but instead accumulated large amounts of LOS-III. Local genomic comparison of the LOS biosynthetic cluster established the presence of a highly disorganized region in Mma7 compared with the standard M strain, characterized by multiple genetic lesions that are likely to be responsible for the defect in LOS-IV production in Mma7. Our results indicate that the glycosyltransferase LosA alone is not sufficient to ensure LOS-IV biosynthesis. The availability of different M. marinum strains allowed us to determine the precise structure of individual LOSs through the combination of mass spectrometric and NMR techniques. In particular, we established the presence of two related 4-C-branched monosaccharides within LOS-II to IV sequences, of which one was never identified before. In addition, we provided evidence that LOSs are capable of inhibiting the secretion of tumor necrosis factor-␣ in lipopolysaccharidestimulated human macrophages. This unexpected finding suggests that these cell wall-associated glycolipids represent key effectors capable of interfering with the establishment of a proinflammatory response.

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Seroreactive species-specific lipooligosaccharides of Mycobacterium mucogenicum sp. nov. (formerly Mycobacterium chelonae-like organisms): identification and chemical characterization

Cited by 19 publications

References 39 publications

rpoB -Based Identification of Nonpigmented and Late-Pigmenting Rapidly Growing Mycobacteria

rpoB -Based Identification of Nonpigmented and Late-Pigmenting Rapidly Growing Mycobacteria

The production of a new extracellular putative long-chain saturated polyester by smooth variants of Mycobacterium vaccae interferes with Th1-cytokine production

Mycobacterium marinum Lipooligosaccharides Are Unique Caryophyllose-containing Cell Wall Glycolipids That Inhibit Tumor Necrosis Factor-α Secretion in Macrophages

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