Seroprevalence of Zika virus among blood donors before the epidemic in Barranquilla, Colombia, 2015-2016.

Bayona-Pacheco, Brayan; Acosta‐Reyes, Jorge; Navarro, Édgar; San-Juan, Homero; Bula, Johan; Baquero, Hernando

doi:10.1590/0001-3765201920180860

Cited by 6 publications

(9 citation statements)

References 26 publications

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“…In Colombia, another South American country, the prevalence of ZIKV was on the order of 2.62% among blood donors in the period from 2015 to 2016 [ 31 ]. The fact that we did not find cases in our study reinforces that since 2018, clinical screening and population awareness have probably allowed this prevalence to decrease among blood-bank donors [ 32 ].…”

Section: Discussionsupporting

confidence: 68%

Prevalence of Dengue, Chikungunya and Zika Viruses in Blood Donors in the State of Pará, Northern Brazil: 2018–2020

et al. 2022

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Arboviruses have been reported over the years as constant threats to blood transfusion recipients, given the high occurrence of asymptomatic cases and the fact that the presence of viremia precedes the onset of symptoms, making it possible that infected blood from donors act as a source of dissemination. This work aims to identify the prevalence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) infection in blood donors during epidemic and non-epidemic periods; classify the donor as symptomatic or asymptomatic; and verify the need to include DENV, CHIKV and ZIKV in the nucleic acid test (NAT) platform in northern Brazil. We investigated 36,133 thousand donations in two years of collection in Northern Brazil. One donor was positive for DENV and one for CHIKV (0.002% prevalence). As the prevalence for arboviruses was low in this study, it would not justify the individual screening of samples from donors in a blood bank. Thus, DENV- and CHIKV-positive samples were simulated in different amounts of sample pools, and both were safely detected by molecular biology even in a pool of 14 samples, which would meet the need to include these three viruses in the routine of blood centers in endemic countries such as Brazil.

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Section: Discussionsupporting

confidence: 68%

Prevalence of Dengue, Chikungunya and Zika Viruses in Blood Donors in the State of Pará, Northern Brazil: 2018–2020

et al. 2022

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“…Samples from local blood banks before the date were tested to estimate the presence of the virus in Colombia. Researchers found samples from June to July 2015 contained antibodies against Zika virus and the seroprevalence grown over time ( Bayona-Pacheco et al, 2019 ), which suggesting that the occurrence of the virus were much earlier than reported.…”

Section: Discussionmentioning

confidence: 99%

Serosurvey for SARS-CoV-2 among blood donors in Wuhan, China from September to December 2019

et al. 2022

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The emerging of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused COVID-19 pandemic. The first case of COVID-19 was reported at early December in 2019 in Wuhan city, China. To examine specific antibodies against SARS-CoV-2 in biological samples before December 2019 would give clues when the epidemic of SARS-CoV-2 might start to circulate in populations. We obtained all 88,517 plasmas from 76,844 blood donors in Wuhan between 1st September and 31st December 2019. We first evaluated the pan-immunoglobin (pan-Ig) against SARS-CoV-2 in 43,850 samples from 32,484 blood donors with suitable sample quality and enough volume. 264 samples from 213 donors were pan-Ig reactive, then further tested IgG and IgM, and validated by neutralizing antibodies against SARS-CoV-2. 213 samples (from 175 donors) were only pan-Ig reactive, 8 (from 4 donors) were pan-Ig and IgG reactive and 43 (from 34 donors) were pan-Ig and IgM reactive. Microneutralization assay showed all negative results. In addition, 213 screened reactive donors were analyzed and didn’t show obviously temporal or regional tendency, but the distribution of age showed a difference compared with all tested donors. Then we reviewed SARS-CoV-2 antibody results from these donors who donated several times from September 2019 to June 2020, partly tested in a previous published study, no one was found a significant increase in S/CO of antibodies against SARS-CoV-2. Our findings showed no SARS-CoV-2 specific antibodies existing among blood donors in Wuhan, China before 2020, indicating no evidence of transmission of COVID-19 before December 2019 in Wuhan, China.

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“…After the emergence of ZIKV in Brazil in 2015, infection by the virus was associated with a significant increase in the number of infants born with microcephaly and birth defects, as well as an increase in neurological disease cases in all age groups, such as myelitis and Guillain-Barre 7 , 8 . Several studies have shown that ZIKV can be transmitted through sex and blood transfusion, which complicates the understanding of the transmission process as infected individuals may not be aware of the transmission potential 9 - 13 . Since ZIKV was declared as a global emergency by the World Health Organization, countries vulnerable to ZIKV emergence should be able to detect and confirm ZIKV cases regardless of whether or not active cases are detected in endemic regions 14 - 16 .…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a vero cell-based suspension method for the detection of Zika virus

Popuche,

Huaman,

Loyola

et al. 2023

Rev Peru Med Exp Salud Publica

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Objective. To develop and validate a cell suspension method using Vero 76 cells for culturing Zika virus (ZIKV) based on infection of detached freshly seeded cells. Material and methods. Three different multiplicities of ZIKV infection were used to develop and compare this novel method to the standard confluent cell monolayer method. In addition, we preliminary validated the cell suspension method using well-characterized ZIKV positive and negative clinical samples. The standard confluent cell monolayermethod was used as the reference method, and viral isolation was confirmed by a ZIKV-specific RT-PCR. We estimated the sensitivity, and its 95% confidence intervals, for the cell suspension method. Also, a technical comparison of the cell suspension method against the cell monolayer method was performed. Results. Our findings suggest that both the viral load and replication of ZIKV were comparable between both monolayer- and suspension-infection methods. Although both methods were suitable for culturing and isolating ZIKV, the cell suspension method was easier, cheaper, and quicker as well as a sensitive isolation technique. The cell suspension method was significantly more sensitive in detecting Zika in inconclusive cases by RT-PCR, with a fourfold increase compared to the confluent cell monolayer method. Conclusion. The cell suspension method has the potential to be an effective method for cultivating and isolating ZIKV and its application is potentially useful in both research and clinical settings.

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Seroprevalence of Zika virus among blood donors before the epidemic in Barranquilla, Colombia, 2015-2016.

Cited by 6 publications

References 26 publications

Prevalence of Dengue, Chikungunya and Zika Viruses in Blood Donors in the State of Pará, Northern Brazil: 2018–2020

Prevalence of Dengue, Chikungunya and Zika Viruses in Blood Donors in the State of Pará, Northern Brazil: 2018–2020

Serosurvey for SARS-CoV-2 among blood donors in Wuhan, China from September to December 2019

Development and validation of a vero cell-based suspension method for the detection of Zika virus

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