Seroprevalence of Trypanosoma cruzi Infection in Pregnant Women Suggests a High Risk for Congenital Transmission in Central Veracruz, Mexico

López‐Monteon, Aracely; Montero, Hilda; González-Constantino, Ruth Sarahi; Limón-Flores, Alberto Y.; Varela-Cardoso, Miguel; Luna-Hernández, Gerardo; Dumonteil, Eric; Ramos-Ligonio, Ángel

doi:10.2478/s11686-020-00197-z

Cited by 3 publications

(4 citation statements)

References 21 publications

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Section: Discussionsupporting

confidence: 85%

“…In Palenque and Poza Rica, two other urban cities in Mexico, rates of 5% and 3.5% were found among pregnant women. Some studies in other regions report rates as high as 17% [40]. Our study reinforces the importance of screening pregnant women for T. cruzi infection, as they have a higher seroprevalence than the general population: A 2005 study using blood bank specimens found a 0.37% seroprevalence in Mexico City [41]; compare this to the 2.12% seroprevalence found in pregnant women [38].…”

Section: Discussionsupporting

confidence: 82%

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Chagas Disease in Pregnant Women from Endemic Regions Attending the Hospital General de Mexico, Mexico City

et al. 2022

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Trypanosoma cruzi infection leads to Chagas disease (CD), a neglected tropical infection of significant public health importance in South and Central America and other, non-endemic, countries. Pregnant women and their children are of particular importance to screen as T. cruzi can be transmitted vertically. The objective of this study was to screen for T. cruzi infection among pregnant women from endemic areas seen at the Hospital General de Mexico for prenatal care, so that they and their children may be quickly connected to CD treatment. Pregnant women were recruited through the hospital prenatal clinic and screened for T. cruzi infection using a series of serological and molecular tests. Of 150 screened patients, mean age 26.8 (SD 6.4), 30 (20.0%) were positive by at least one diagnostic test. Of these, only nine (6%) were positive as determined by PCR. Diagnosis of chronic CD is difficult in endemic places like Mexico due to the limitations of current commercially available diagnostic tests. Further evaluation of diagnostic performance of various assays could improve current CD diagnostic algorithms and proper care management in these regions. Genetic variability in the parasite may also play a role in the differing assay performances seen in this study, and this may be a valuable avenue of further research.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 82%

Chagas Disease in Pregnant Women from Endemic Regions Attending the Hospital General de Mexico, Mexico City

et al. 2022

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“…The T. cruzi infections transmitted by blood transfusion [21][22][23], by oral route [24], and by accident in hospitals and research laboratories [25,26]. Moreover, the congenital, sexually transmitted T. cruzi infection from males and females to naive mates could play an ongoing pandemic role [6,[27][28][29][30][31][32][33][34][35][36][37][38]39].…”

Section: Introductionmentioning

confidence: 99%

“…The prevalence of Chagas disease, in the absence of systematic methods to obtaining epidemiologic data for the meta-analysis, has been assumed by the extrapolation of the information from independent field studies carried out in various counties of the Latin America [2,[23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42]. The systematic, country-wide survey that was conducted in Brazil, during the years 1975-1980, employed the indirect immunofluorescence method for the search of the T. cruzi antibody eluted from a drop of blood serum sample collected in filter paper.…”

Section: Introductionmentioning

confidence: 99%

The Trypanosoma cruzi kinetoplast DNA minicircle sequences transfer biomarker of the multidrug treatment of Chagas disease

Teixeira

Sousa

Gomes

et al. 2021

Preprint

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BackgroundThe Trypanosoma cruzi infection renders the transfer of the mitochondrion kinetoplast DNA minicircle sequences into the host’s genome. The Aves are refractory to the infection, but chicks hatched from the T. cruzi inoculated eggs integrate the DNA minicircle sequences into the germ line cells. Rabbits, mice and chickens with the minicircle sequences mutations develop the Chagas cardiomyopathy and the DNA transfer underpins the heart disease.MethodologyThe PCR with the specific primer sets revealed the Protist nuclear DNA and the kinetoplast DNA in the agarose gels bands probed with the radiolabel specific sequences from tissues of the T. cruzi-infected rabbits and of the mice. A target- primer TAIL-PCR amplification employing primer sets from the chickens, rabbits and mice, in combination with primer sets from the the T. cruzi kinetoplast minicircle sequences was used. This approach led us to disclose the integration sites of the kinetoplast DNA biomarker, then, used to monitor the effect of multidrug treatment of the T. cruzi infected mice.Principal findingsThe Southern hybridization, clone and sequence of the amplification products revealed the DNA minicircle sequences integrations sites in the LINE transposable elements. An array of inhibitors of eukaryote cells division was used to arrest the DNA transfer. It was shown that nine out of 12 inhibitors prevented the kinetoplast DNA integration into the macrophage genome. The multidrug treatment of the acutely T. cruzi-infected mice with Benznidazole, Azidothymidine and Ofloxacin lessened circa 2.5-fold the rate of the minicircle sequences integrations in the mouse genome and inhibited the rejection of the target heart cells.Conclusion and significanceThe T. cruzi mitochondrion kinetoplast minicircle sequences transfer driven pathogenesis of Chagas disease is an ancient Cross-Kingdom DNA phenomenon of evolution and, therefore, paradigm research with effective purposing inhibitors is needed.Authors summaryChagas disease is considered the main cause of human heart failure in the Western Hemisphere. The treatment of the clinically manifested Chagas heart disease is considered unsatisfactory. Perhaps the most important problem in the field of Chagas disease is determination of the pathogenesis of the target heart cells lysis. We showed the transfer of the T. cruzi kDNA minicircle sequences into the genome of rabbits and mice, and to Bird refractory to the infections. The inoculation of a few T. cruzi in the fertile chicken eggs renders the kDNA sequences integration in the stem cells. Interestingly, the chicks that hatched retain the kDNA and develop the Chagas-like cardiomyopathy indistinguishable to that in the rabbits and mice. This result prompted the multidrug treatment of the Chagas heart disease with inhibitors of the eukaryotic cells division. We showed that nine out of 12 inhibitors prevented the transfer of the kDNA mutations into the macrophage genome, and that the treatment of the acutely T. cruzi-infected mice with Benznidazole + Ofloxacin + Azidothymidine lowered circa 2.5-fold the rate of the mutations in the chromosomes. These findings translated to the pathology showing inhibition of the heart lesions in the treated T. cruzi-infected mice. We suggest purposing new inhibitors should be tested to overturning the Chagas heart disease.

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Diversity of Trypanosoma cruzi parasites infecting Triatoma dimidiata in Central Veracruz, Mexico, and their One Health ecological interactions

Murillo-Solano

Ramos-Ligonio

López‐Monteon

et al. 2021

Infection, Genetics and Evolution

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Seroprevalence of Trypanosoma cruzi Infection in Pregnant Women Suggests a High Risk for Congenital Transmission in Central Veracruz, Mexico

Cited by 3 publications

References 21 publications

Chagas Disease in Pregnant Women from Endemic Regions Attending the Hospital General de Mexico, Mexico City

Chagas Disease in Pregnant Women from Endemic Regions Attending the Hospital General de Mexico, Mexico City

The Trypanosoma cruzi kinetoplast DNA minicircle sequences transfer biomarker of the multidrug treatment of Chagas disease

Diversity of Trypanosoma cruzi parasites infecting Triatoma dimidiata in Central Veracruz, Mexico, and their One Health ecological interactions

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