Seroprevalence of neutralizing antibodies to human adenovirus type 5 in healthy adults in China

Abstract

Human adenoviruses have been used to develop candidate HIV vaccine vectors, but a major obstacle to the use of vectors derived from Ad5 and other common serotypes is the high prevalence of neutralizing antibodies (NAbs) in humans. Prior studies have reported a prevalence of Ad5 between 60% and 70% in Europe and US, and up to 98% in surveyed African and Asian (Thai) tropical countries. However, few studies have reported on the Ad5 prevalence in Chinese populations. In this study, a total of 1,250 healthy adult serum samples from six administratively separate regions of China were screened by high-throughput luciferase-based virus neutralization assays. Results showed that overall 72% of healthy adults were Ad5 seropositive, and 46.4% with baseline Ad5 NAb titers of >200 in China. Comprehensive analysis by geographical region and age showed that the seroprevalence of Ad5 in southern China such as Guangxi was higher than that in other regions. Geographical differences and climate were considered as the major factors affecting the titer levels of Ad5 in healthy adults. In addition, no apparent gender and ethnic difference was found in any group classified according to region, age, or NAb titer. The present study may provide useful insights for the future development of Ad5-based vaccines and gene therapy.

Prevalence of serum neutralizing antibodies to adenovirus type 5 (Ad5) and 41 (Ad41) in children is associated with age and sanitary conditions

“…International serosurveys demonstrate that the seroprevalence of Ad5 NAb varies in different geographic regions although the reason for that is still unknown [22,23]. Prevalence rate of Ad5 NAb in China was reported to be 60-82% in healthy adults, which slightly fluctuated according to different geographic locations and age [24][25][26]. Our results showed that the seroprevalence of Ad5 NAb in adults in Xining was 75.7%, which was consistent with the published investigations [24][25][26].…”

“…Prevalence rate of Ad5 NAb in China was reported to be 60-82% in healthy adults, which slightly fluctuated according to different geographic locations and age [24][25][26]. Our results showed that the seroprevalence of Ad5 NAb in adults in Xining was 75.7%, which was consistent with the published investigations [24][25][26]. Yu and the colleagues determined the Ad2 and Ad5 NAb titers of 274 children below 12 years of age in Changchun (the capital city of Jilin province, China), and found that Ad5 NAb prevalence rate was age dependent and the overall prevalence of Ad5 NAb was 43%.…”

Seroprevalence of neutralizing antibodies to human adenoviruses type‐5 and type‐26 and chimpanzee adenovirus type‐68 in healthy Chinese adults

“…In the present study, anti‐AdHu5 neutralizing antibodies were detected in 73.1% of Chinese subjects. Yu et al [2012] showed a similar result that the positive rate of anti‐AdHu5 neutralizing antibodies in healthy adults from the other six regions in China is 72%.…”

A serological survey of human adenovirus serotype 2 and 5 circulating pediatric populations in Changchun, China, 2011

“…However, our prior epidemiological study of Ad5 NAbs showed that areas in southern China may have a higher seroprevalence of Ad5 than in other regions [12]. The objective of this current study was to determine the prevalence of NAbs against both Ad2 and Ad5 in pediatric populations in northeast China.…”