Seroprevalence of Neospora caninum in dairy cattle ranches with high abortion rate: Special emphasis to serologic co-existence with Toxoplasma gondii, Brucella abortus and Listeria monocytogenes

Yıldız, Kader; Kul, Oğuz; Babür, Cahit; Kılıç, Selçuk; Gazyağci, Aycan Nuriye; Çelebі, Bekіr; Gürcan, İsmayil Safa

doi:10.1016/j.vetpar.2009.06.004

Cited by 40 publications

(30 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This suggests the likelihood of even higher abortion rates in buffalo attributed to coexistence of pathogens. Our findings on co-infection are in compliance with a previous study (26) on dairy cattle in Turkey demonstrating 13.8% co-existence of N. caninum and B. abortus. The widespread occurrence of N. caninum co-infection in cattle with abortifacient pathogens has been recently described in Punjab (Pakistan) (27).…”

supporting

confidence: 93%

Co-infection of water buffaloes in Punjab, Pakistan, with Neospora caninum and Brucella abortus

Nasir¹,

Ashraf²,

Shakoor³

et al. 2014

Turk J Vet Anim Sci

View full text Add to dashboard Cite

Neosporosis and brucellosis are 2 well-known diseases causing bovine abortion. This study determined co-infection of Neospora caninum and Brucella abortus in buffaloes for the first time in Pakistan. A monoclonal antibody based competitive ELISA was used to test buffalo sera (n = 312) for N. caninum specific antibodies, while rose bengal plate test assessed B. abortus antibodies. N. caninum and B. abortus prevalence was 42.8% (±6.7, 95% C.I.) and 12.2% (±3.6, 95% C.I.), respectively. Buffaloes >3 years old exhibited higher prevalence than younger ones. Co-infection was particularly significant as 13.2% of Neospora infected buffaloes were also Brucella infected with an overall co-existence of 12.2%, suggesting higher abortion risk in such animals than in those infected with either one of the pathogens. Proper disposal of aborted fetuses and placentae, and testing and culling infected animals are suggested to impede transmission of pathogens.

show abstract

supporting

confidence: 93%

Co-infection of water buffaloes in Punjab, Pakistan, with Neospora caninum and Brucella abortus

Nasir¹,

Ashraf²,

Shakoor³

et al. 2014

Turk J Vet Anim Sci

View full text Add to dashboard Cite

show abstract

“…In the present study, from the 41 dairy cows that aborted, 51.2% were seropositive for N. caninum; comparatively lower seropositivity were reported for N. caninum in other studies, varying between 6.3 to 28.6% (MEERSCHMAN et al, 2002;KOIWAI et al, 2005;YILDIZ et al, 2009). In this study, dams that were more than 36 months of age had a higher risk of being seropositive; similar findings were previously described Diagnosis of Neospora caninum abortions in dairy cows Mummified fetuses (mum) did not have their tissues and blood tested, and age determined.…”

Section: Discussioncontrasting

confidence: 52%

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Macedo

Miura

et al. 2017

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.Keywords: Neosporosis, fetuses, lesion, serology, cattle. ResumoO objetivo deste estudo foi avaliar a ocorrência de N. caninum associado a abortamentos em vacas de leite do estado de Santa Catarina, sul do Brasil pelo uso das técnicas de ELISA (HerdCheck, IDEXX), reação em cadeia pela polimerase (PCR) e imunohistoquímica (IHC). O sangue das vacas leiteiras que abortaram, bem como, o líquido intratorácico e amostras de tecidos (cérebro, coração, fígado e pulmão) de seus fetos foram coletados e usados para sorologia, PCR (Np21+ e Np6+), e IHC. Vinte e uma vacas (51,2%) de um total de 41, bem como, oito fetos (26,7%) de um total de 30 foram positivos no ELISA (IDEXX) para N. caninum. As vacas > 36 meses de idade tiveram um maior risco de serem soropositivas do que os animais mais jovens. PCR e IHC revelaram que 38,8% e 25,0% dos fetos foram positivos para N. caninum, respectivamente para cada um dos testes. As vacas soropositivas tiveram uma maior frequência de fetos que também foram positivos no fluído intratorácico, na PCR ou na IHC. Em resumo, o presente estudo observou uma alta frequência de N. caninum em abortos de vacas leiteiras na região estudada, com maior prevalência de N. caninum em vacas com mais de 36 meses de idade. Além disso, sorologia, PCR e IHC deveriam ser utilizadas conjuntamente para melhor diagnóstico de neosporose em bovinos.

show abstract

“…Carriage rate for animals was similar to that of humans, with some differences depending on the species and a little higher rate during indoor season, as compared to animals on pasture (Iida et al 1991). On the other hand, Yildiz et al (2009) determined seroprevalence of Neospora caninum in dairy cattle with higher abortion rates and coexistence of N. caninum with L. monocytogenes antibodies and indicated that N. caninum seroprevalence was 10.77% (60/557) while the co-existence rate of N. caninum seropositivity with L. monocytogenes was 42.85% (162/378). Moreover, AbdEl-Malek, et al ( 2010) examined 28 stool cultures from hospitalized children with diarrheal disease in Assiut Univ.…”

Section: Discussionmentioning

confidence: 68%

Detection of Circulating Immunoglobulins in Serum of Sheep Naturally Infected with Chlamydia Trachomatis and Listeria Monocytogenes in Two Egyptian Provinces

Zidan¹,

Tarabees²,

Dahy³

2014

AJVS

View full text Add to dashboard Cite

C. trachomatis, L. monocytogenes, ELISAChlamydia trachomatis and Listeria monocytogenes are important reproductive tract pathogens in a wide variety of animals including sheep. In humans, chronic or repeated infection of the female genital tract with Chlamydia trachomatis has been identified as a significant factor in the development of occlusive infertility or increased risk of ectopic pregnancy. The aim of the current study is to detect circulating immunoglobulins in serum of sheep naturally infected with Chlamydia trachomatis and Listeria monocytogenes in two Egyptian provinces. A total of 82 serum samples from 4 sheep flocks in two Egyptian provinces were examined with enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies of Chlamydia trachomatis and Listeria monocytogenes. The results of ELISA revealed the detection of antibodies against the two pathogens at a very high percentage (98.78% and 100% for Chlamydia trachomatis and Listeria monocytogenes, respectively) despite of absence of elevated abortion rates in the examined sheep flocks. It could be concluded that chlamydial and listerial infections occur frequently in Egyptian sheep flocks, even in the absence of elevated abortion rates and there is possible risk to pregnant women in rural areas where chlamydial and listerial infections in farm animals are widespread. Also, ewes that experienced Chlamydia trachomatis or Listeria monocytogenes induced abortion provide a unique opportunity to study the host: parasite dynamic as it relates to persistent infection. This natural model of persistent infection may, in some ways, be superior to more contrived models in which the chlamydial or listerial isolate is not a normal reproductive pathogen of the study animal. Thus, the study of persistent Chlamydial or Listerial infection in sheep may be used for the benefit of both human and veterinary medicine.

show abstract

Seroprevalence of Neospora caninum in dairy cattle ranches with high abortion rate: Special emphasis to serologic co-existence with Toxoplasma gondii, Brucella abortus and Listeria monocytogenes

Cited by 40 publications

References 26 publications

Co-infection of water buffaloes in Punjab, Pakistan, with Neospora caninum and Brucella abortus

Co-infection of water buffaloes in Punjab, Pakistan, with Neospora caninum and Brucella abortus

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Detection of Circulating Immunoglobulins in Serum of Sheep Naturally Infected with Chlamydia Trachomatis and Listeria Monocytogenes in Two Egyptian Provinces

Contact Info

Product

Resources

About