Seroprevalence of Leptospira spp. in Horses of Distinct Climatic Regions of Punjab, Pakistan

Sohail, Muhammad; Khan, Muhammad Sarwar; Avais, Muhammad; Zahoor, Muhammad; Ijaz, Muhammad; Ullah, Aman; Fatima, Zahida; Naseer, Omer; Khattak, Irfan; Ali, Sadaqat

doi:10.1016/j.jevs.2016.01.007

Cited by 10 publications

(9 citation statements)

References 30 publications

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“…In this study buffaloes of district Rajanpur presented higher prevalence of anitleptospiral antibodies than the animals from Muzaffargarh district. These types of results are in agreement with the findings of Sohail et al (2016) who also have reported significant difference of Leptospira sero-prevalence and study area in horses of Pakistan. The difference in prevalence in both of the study districts might be due to different production and rearing systems.…”

Section: Discussionsupporting

confidence: 92%

“…This study was conducted in buffaloes of flood hit zones of Pakistan, and flood water is supposed to be positively associated with the occurrence of disease. These findings are justified by Bandino et al (2015) and Sohail et al (2016) as they proposed floods and rain fall both strongly associated with sero-prevalence of Leptospira.…”

Section: Discussionmentioning

confidence: 75%

“…Letospira's sero-prevalence has been reported in human as well as in domestic animals of Pakistan by rapid microscopic slide agglutination test (Ahmad, 1987). There are also few studies which have reported this pathogen in dogs (Saleem et al, 2013a), horses (Sohail et al, 2016), bovine (Ijaz et al, 2018a, b;Islam et al, 2019) and human population (Saleem et al, 2013b;Sohail et al, 2018) of Pakistan. This study shows the association of leptospira sero positivity with reproductive parameters which is supported by Boqvist et al (2002).…”

Section: Discussionmentioning

confidence: 99%

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Seroprevalence of Leptospirosis and Its Association With Reproductive and Productive Parameters From Buffalo Population of Rajanpur and Muzaffargarh Districts of Pakistan

2020

THE JAPS

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Leptospirosis is one of the important infectious diseases adversely affecting the fertility and reproductive parameters of domestic animals. The current study was aimed to investigate the sero-prevalence of leptospirosis and its association with reproductive and productive parameters from buffalo population of Rajanpur and Muzaffargarh districts of Punjab, Pakistan. A total of 386 buffalo serum samples were analyzed for anti-leptospira antibodies through indirect ELISA using "Anti-Leptospira IgG kit". The information regarding disease determinants were captured in questionnaire. Univariable and multivariable logistic regression models were used to correlate the potential risk factors with the disease dynamics. The results revealed an overall 12.69% prevalence of leptospirosis in both of the study districts, with higher percentage reported in district Rajanpur followed by Muzaffargarh presenting 20.72 and 04.66% prevalence at significant difference (p≤0.05), respectively. The univariable analysis found district, estrus repetition, body condition score and history of retained placenta as significant candidates (p ≤ 0.05) while, multivariable logistic regression presented district, gender of animal and estrus repetition as the key risk factors (Odds Ratio>1). The study concluded that leptospirosis is prevalent in study districts and there is association of assumed risk factors with disease dynamics, which demands early installment of control strategies before the disease become unleashed.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

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Seroprevalence of Leptospirosis and Its Association With Reproductive and Productive Parameters From Buffalo Population of Rajanpur and Muzaffargarh Districts of Pakistan

2020

THE JAPS

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“…Despite high rates of seropositivity among animals in Pakistan (2,20), to date, there have been no data on the molecular studies of leptospirosis in horses or in any mammalian host. This investigation reports the first molecular evidence of equine leptospirosis in Pakistan, where previous serological reports have revealed a prevalence of over 40% (21). The aim of this investigation was to detect the presence of leptospiral DNA in horse blood samples and to analyse genetic diversity of the Leptospira in the country.…”

Section: Discussionmentioning

confidence: 95%

“…In Pakistan, serological studies on equine leptospirosis showed a prevalence of up to 44% in some areas of the country, which is quite alarming (21). All previous studies reporting Leptospira in Pakistan were based on serological testing (2,20).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterisation of Leptospira strains in Pakistan

Sohail

Khan

Avais

et al. 2016

Journal of Veterinary Research

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Introduction: Leptospirosis affects a wide range of mammals, humans, and even a few poikilothermic animal species. In Pakistan, serological studies of equine leptospirosis have reported a prevalence of over 40%, but no study has ever been conducted towards molecular detection of Leptospira in horses. Material and Methods: Blood samples from 128 horses were screened using ELISA and 41 positive samples were examined for the presence of leptospiral DNA using specific primers for 16S rRNA gene. Results: Out of 41 tested samples, 20 samples were found to be PCR-positive, revealing a fragment of 306 bp after gel electrophoresis. Sequencing and phylogenetic analysis of positive samples revealed circulation of pathogenic Leptospira spp. in Pakistani horses. No evidence of circulation of intermediate species was found in this study. Conclusion: This study reports the first molecular evidence of equine leptospirosis in Pakistan and lays ground for further research in this area. It also confirms the efficiency of 16S rRNA for the diagnosis of equine leptospirosis.

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