Seroprevalence of <i>Ehrlichia canis</i> antibodies among dogs in Turkey

Batmaz, Hasan; Şentürk, Sezgin; Yılmaz, Zeki; Harrus, Shimon; Nevo, Ettay; Waner, Trevor

doi:10.1136/vr.148.21.665

Cited by 22 publications

(20 citation statements)

References 7 publications

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“…Analysis on variables such as sex, breed and age did not show any significant differences (p < 0.05) among them, although the prevalence was higher in male dogs (18.2%), which is in agreement with Rodriguez-Vivas et al (2005) and Solano-Gallego et al (2006) but not with Batmaz et al (2001). Furthermore, the frequency of seropositivity was higher in dogs aged 1-3 years (18.3%) than in other age groups.…”

Section: Discussionsupporting

confidence: 69%

“…The higher seroprevalence found in Turkey (21%) and Israel (30%) (BATMAZ et al, 2001;BANETH et al, 1996, respectively) might be associated with different climatic conditions, the severity of tick infestation and use of preventive or therapeutic anti-ectoparasite applications.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, several strains of E. canis such as the Thai, Turkish, Venezuelan and Greek strains have been detected using PCR in different geographical regions. The seroprevalence of E. canis among dogs in the Middle East was reported to be 21% in Turkey (BATMAZ et al, 2001) and 30% in Israel (BANETH et al, 1996). Recent surveys in the cities of Kerman and Ahvaz in Iran revealed that 14.63% and 9.6% of dogs were seropositive for E. canis respectively (AKHTARDANESH et al, 2010;AVIZEH et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Molecular and serological detection of Ehrlichia canis in naturally exposed dogs in Iran: an analysis on associated risk factors

Maazi

Malmasi

Shayan

et al. 2014

Rev. Bras. Parasitol. Vet.

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The general aim of this study, which was conducted for the first time in Iran, was to evaluate the seroprevalence and geographical distribution of Ehrlichia canis in a dog population in Iran, followed by molecular confirmation using PCR and sequencing. Blood samples were collected from 240 dogs in different areas of Alborz and Tehran Provinces and initially analyzed using the immunofluorescent antibody (IFA) test to detect anti-Ehrlichia canis IgG antibodies. Subsequently, nested PCR was performed based on a fragment of the 16S rRNA gene of E. canis on serologically positive samples. The results showed that 40/240 dogs (16.6%) presented anti-Ehrlichia canis IgG antibodies and that nine of the blood samples from the 40 seropositive dogs (22.5%) contained E. canis DNA, which was confirmed by sequencing. The seroprevalence of E. canis tended to be higher in purebred, one to three-year-old male dogs living in the Plain zone, in rural areas; however, this difference was not statistically significant.Keywords: Ehrlichiosis, Ehrlichia canis, IFA, nested PCR, dogs, Iran. ResumoO objetivo geral deste estudo, que foi feito pela primeira vez no Irã, foi avaliar a soroprevalência e distribuição geográfica de Ehrlichia canis em população de cães no Irã, seguida da confirmação molecular por meio de PCR seguida de sequenciamento. Amostras de sangue de 240 cães de diferentes áreas das Províncias de Alborz e Teerã foram coletadas e, inicialmente, analisadas pelo Reação de Imunofluorescência (IFA) para detecção de anticorpos IgG anti-Ehrlichia canis Subsequentemente, uma reação do tipo nested PCR baseada em um fragmento do gene 16S rRNA de E. canis foi realizada nas amostras sorologicamente positivas. Os resultados mostraram que 40/240 cães (16,6%) apresentaram anticorpos IgG anti-Ehrlichia canis e nove (22,5%) das amostras de sangue dos 40 cães soropositivos continham DNA de E. canis, confirmado por sequenciamento. A soroprevalência de E. canis, embora não estatisticamente significativa, mostrou uma tendência em se apresentar maior em cães machos com 1-3 anos, de raça pura, que vivem em zonas planas e áreas rurais.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular and serological detection of Ehrlichia canis in naturally exposed dogs in Iran: an analysis on associated risk factors

Maazi

Malmasi

Shayan

et al. 2014

Rev. Bras. Parasitol. Vet.

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“…Algunos estudios no muestran diferencias respecto al género (23); sin embargo, otros estudios evidencian mayor positividad en machos que en hembras, contrario a lo observado en el presente estudio (24). Esto implica la necesidad de evaluar este posible factor asociado en cada población particular.…”

Section: Discussionunclassified

Seroprevalencia de Ehrlichia canis en perros con sospecha de infección por patógenos transmitidos por garrapatas en Medellín, 2012-2014

Yarce¹,

Osorio²,

Arias³

2015

Rev. Med. Vet.

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ResumenEn Colombia son exiguas las investigaciones sobre ehrlichiosis canina y en Medellín son nulas. Esta investigación tuvo como objetivo determinar la seroprevalencia de Ehrlichia canis y su distribución según sexo, edad, raza y tamaño en caninos diagnosticados en un laboratorio veterinario de Medellín, entre 2012 y 2014. Para ello se diseñó un estudio transversal en 781 caninos. Se calculó la seroprevalencia global de infección y específica según sexo, grupo de edad, tamaño y raza del canino. En el análisis bivariado se usaron las pruebas Z, chi cuadrado de Pearson y U de Mann-Whitney. En el análisis multivariado se hizo regresión logística binaria. Se incluyeron 57 razas, de las cuales las más frecuentes fueron criollos, labradores y french poodle; 54,9 % eran machos y 56,9 %, adultos. La prevalencia global de la infección fue 24,8 %; las mayores seroprevalencias específicas se observaron en las hembras (25,9 %), los seniles (29,7 %) y los pertenecientes a razas grandes (27,6 %). El riesgo de infección en adultos y seniles fue 2 veces el hallado en cachorros; la probabilidad de infección en los cocker spaniel fue 6,4 veces la hallada en los bulldog francés; el riesgo de infección en lobo siberiano, pug y labrador fue 7,8, 5,5 y 4,1 veces lo observado en los bulldog. La alta seroprevalencia de ehrlichiosis canina y la identificación de los caninos adultos, seniles y las razas cocker spaniel, lobos siberianos, pug y labradores como las de mayor riesgo evidencian la necesidad de formular programas de prevención y atención de esta infección en la ciudad.Palabras clave: Ehrlichia canis, enfermedades de los perros, ensayo de inmunoadsorción enzimática, prevalencia. Seroprevalence of Ehrlichia canis in Dogs with Suspected Infection by Tick-Borne Pathogens in Medellín, 2012-2014 AbstractResearch is meager on canine ehrlichiosis in Colombia and it is absent in Medellín. This research aims to determine the seroprevalence of Ehrlichia canis and its distribution by sex, age, race and size in dogs diagnosed in a veterinary laboratory in Medellín, between 2012 and 2014. To the effect, a cross-sectional study was designed in 781 dogs. Overall seroprevalence of infection and specific by sex, age, size, and canine breed were calculated. In the bivariate analysis, Z test, Pearson's chi-square test, and the Mann-Whitney U test were used. In the multivariate analysis, binary logistic regression was performed. 57 races were included, of which the most frequent were Creoles, Labradors, and French poodles; 54.9% were males, and 56.9% were adults. Overall prevalence of infection was 24.8%; highest specific seroprevalences were observed in females (25.9%), senile dogs (29.7%), and those belonging to large breeds (27.6%). The risk of infection in adult and senile dogs was two times higher than in puppies; the probability of infection was 6.4 times higher in cocker spaniels than in French Seroprevalencia de Ehrlichia canis en perros con sospecha de infección por patógenos transmitidos por garrapatas en

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“…CME'nin teşhisinde son zamanlarda PCR tabanlı moleküler teknikler kullanılmış olup E. canis'in farklı genotiplerinin ayrımında en yaygın 16S rRNA gen bölgesinin kullanıldığı bildirilmiştir (49). Türkiye'de CME üzerine yapılan çalışmalar oldukça sınırlı olup bunlar ağırlıklı olarak antikor saptamaya yönelik olmuştur (2,6,9,19,21,44,50). Bunun yanında, Türkiye'de konvansiyonel ve nested PCR teknikleri kullanılarak CME üzerine yapılmış birkaç moleküler çalışma da rapor edilmiştir (3).…”

Section: Tartışma Ve Sonuçunclassified

Köpeklerde kene kaynaklı bazı protozoon ve rickettsial enfeksiyonların Real Time PCR ile araştırılması ve saptanan izolatların moleküler karakterizasyonları

Önder; İNCİ

2014

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu canis vogeli ve B. gibsoni izolatlarının 18S rRNA gen bölgesine ait sekanslarının dünyadaki aynı türden diğer izolatlarla ikili kıyaslamalarında %1,4±0,2, %0,3±0,2 ve %0,9±0,3 genetik farklılık saptanmıştır. E. canis için 16S rRNA gen bölgesi yönünden üç izolatın kendi aralarında %100 identik olduğu, dünyadaki izolatlarla ise %0,1 farklılık gösterdiği; A. phagocytophilum için ankA gen bölgesi yönünden üç izolatın kendi aralarında %99,8±0,2 identik olduğu, dünyadaki izolatlarla ise %0,9±0,3 farklılık gösterdiği; H. canis için 18S rRNA geni yönünden iki izolatın kendi aralarında %100 identik olduğu, dünyadaki izolatlarla ise %0,2±0,1 farklılık gösterdikleri tespit edilmiştir. Sonuç olarak bu çalışmayla Kayseri yöresinde köpeklerde kene kaynaklı protozoon ve rickettsial enfeksiyonların moleküler prevalansları saptanmış ve enfeksiyonlara yol açan türlerin çeşitli gen bölgeleri analiz edilerek moleküler karakterizasyonları yapılmıştır. Tüm izolatların Genbank kayıtları gerçekleştirilmiştir.Anahtar sözcükler: Anaplasmosis, babesiosis, ehrlichiosis, hepatozoonosis, köpek, moleküler karakterizasyon, Real Time PCR. B. gibsoni, A. phagocytophilum, H. canis, and B. canis vogeli was detected as 14.5%, 12.0%, 9.0%, 7.8%, 5.3%, and 2.3%, respectively while B.canis rossi was not detected in the examined samples. 182 (89.7%) out of the 400 samples were found to be infected with a single parasite species, and 21 (10.3%) were found to be infected with two species. According to the pairwise comparisons of 18S rRNA gene region of the isolates under B. canis canis, B.canis vogeli and B. gibsoni, 1.4±0.2%, 0.3±0.2%, and 0.9±0.3% genetic distance were detected with the other similar isolates from the world, respectively. Based on the 16S rRNA gene sequence alignments, 100% identity was found among the 3 isolates of E. canis while 0.1% genetic difference was determined with the isolates from the world. With respect to ankA gene region of A. phagocytophilum, 99.8±0.2% identity and 0.9±0.3% genetic difference were found among the 3 isolates obtained from Kayseri region and the isolates from the world, respectively. The 2 H. canis isolates were showed 100% identity to each other and 0.2±0.1% genetic difference were determined with the other isolates from the world with respect to 18S rRNA gene region. In conclusion, the molecular prevalence of tick-borne protozoon and rickettsial infections in dogs in Kayseri region was determined and the molecular characterizations of the obtained isolates were performed by analyzing the various gene regions in this study. All isolates were recorded to GenBank.

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Seroprevalence of Ehrlichia canis antibodies among dogs in Turkey

Abstract: Human lymphadenitis due to Corynebacterium pseudotuberculosis; report of 10 cases from Australia and review. Clinical Infectious Diseases 24, 185-191 ROBINS, R. (I1991) Focus oncaseous lymphadenitis. State Veterinary Journal

Cited by 22 publications

References 7 publications

Molecular and serological detection of Ehrlichia canis in naturally exposed dogs in Iran: an analysis on associated risk factors

Molecular and serological detection of Ehrlichia canis in naturally exposed dogs in Iran: an analysis on associated risk factors

Seroprevalencia de Ehrlichia canis en perros con sospecha de infección por patógenos transmitidos por garrapatas en Medellín, 2012-2014

Köpeklerde kene kaynaklı bazı protozoon ve rickettsial enfeksiyonların Real Time PCR ile araştırılması ve saptanan izolatların moleküler karakterizasyonları

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