Seroprevalence of human metapneumovirus (hMPV) in the Canadian province of Saskatchewan analyzed by a recombinant nucleocapsid protein‐based enzyme‐linked immunosorbent assay

Liu, Li; Bastien, Nathalie; Sidaway, F.; Chan, Edward Wai Chi; Li, Yan

doi:10.1002/jmv.20799

Cited by 24 publications

(24 citation statements)

References 21 publications

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“…Several studies have reported high seroprevalences based on enzyme-linked immunosorbent assays (ELISAs) (68,145,176,262) or microneutralization assays (77,153). ELISA detection of HMPV is generally performed with in-house assays, because assay kits are currently not commercially available.…”

Section: Fluorescent-antibody Staining and Elisamentioning

confidence: 99%

Human Metapneumovirus: Lessons Learned over the First Decade

Schildgen

Hoogen²,

Fouchier³

et al. 2011

Clin Microbiol Rev

182

187

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SUMMARY It has been 10 years since human metapneumovirus (HMPV) was identified as a causative agent of respiratory illness in humans. Since then, numerous studies have contributed to a substantial body of knowledge on many aspects of HMPV. This review summarizes our current knowledge on HMPV, HMPV disease pathogenesis, and disease intervention strategies and identifies a number of areas with key questions to be addressed in the future.

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Section: Fluorescent-antibody Staining and Elisamentioning

confidence: 99%

Human Metapneumovirus: Lessons Learned over the First Decade

Schildgen

Hoogen²,

Fouchier³

et al. 2011

Clin Microbiol Rev

182

187

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“…More recently, human metapneumovirus (hMPV), a Pneumovirus belonging also to Paramyxoviridae family, has been isolated from children hospitalized with acute respiratory infections in The Netherlands and since, the virus has been reported worldwide [3-5]. Symptoms associated with hMPV infection are very similar to those resulting from hRSV infection, ranging from common cold to severe lower respiratory tract infections, including bronchiolitis and pneumonia.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of human respiratory syncytial virus and human metapneumovirus in healthy population analyzed by recombinant fusion protein-based enzyme linked immunosorbent assay

Sastre¹,

Ruiz²,

Schildgen

et al. 2012

Virol J

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Background Human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) are two of the most frequent respiratory pathogens that circulate worldwide. Infection with either virus can lead to hospitalization of young children, immunocompromised people and the elderly. A better understanding of the epidemiological aspects, such as prevalence of these viruses in the population will be of significant importance to the scientific community. The aim of this study was to gain some detailed knowledge on the humoral immune response to both viruses in different populations of individuals. Findings The fusion protein (F) of hRSV and hMPV was expressed in the baculovirus and Escherichia coli systems, respectively, and used as antigen in two independent enzyme-linked immunosorbent assays (ELISAs) for detection of specific antibodies in human sera. The seroprevalence of each virus in a large cohort of individuals with ages ranging from 0 to 89 years old was determined. Although the general distribution of the antibody response to each virus in the different age group was similar, the prevalence of hRSV appeared to be higher than that of hMPV in most of them. The group of children with ages between 0 and 2 showed the highest seronegative rates. After this age, an increase in the antibody response was observed, most likely as the result of new infections or even due to reinfections. Conclusions The use of these specific F-ELISAs in seroepidemiological studies might be helpful for a better understanding of the human antibody response to these viruses.

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“…The N protein is the most abundant viral protein and elicits a strong and long-lasting humoral immune response following acute infection (12, 13). Recombinant nucleocapsid (recN) protein has been used successfully to replace whole virus antigen in diagnostic serological assays for hRSV and hMPV by us and others (14,15).In a previous serologic study to assess the etiology of febrile respiratory illness in Bangladeshi children, we observed an unusually high number of increases titers of antibody to hRSV and hMPV (unpublished data). Although coinfections with hRSV and hMPV could explain this observation, coinfections could not be confirmed by reverse transcription-PCR (RT-PCR) of coincidently collected respiratory specimens from these children, and only limited cocirculation of these viruses was observed during the study period (9).…”

mentioning

confidence: 77%

“…However, close examination of the Western blot image presented in Fig. 2A of their manuscript shows a faint band of the expected size in lane 6, demonstrating possible reactivity between N protein [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] antisera and hRSV A2 lysate. We suspect that the use of hRSV recN protein in our assays increased sensitivity over whole-virus antigen and may account for this difference in results.…”

Section: Discussionmentioning

confidence: 99%

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Serologic Cross-Reactions between Nucleocapsid Proteins of Human Respiratory Syncytial Virus and Human Metapneumovirus

et al. 2015

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Human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) share virologic and epidemiologic features and cause clinically similar respiratory illness predominantly in young children. In a previous study of acute febrile respiratory illness in Bangladesh, we tested paired serum specimens from 852 children presenting fever and cough for diagnostic increases in titers of antibody to hRSV and hMPV by enzyme immunoassay (EIA). Unexpectedly, of 93 serum pairs that showed a >4-fold increase in titers of antibody to hRSV, 24 (25.8%) showed a concurrent increase in titers of antibody to hMPV; of 91 pairs showing an increase to hMPV, 13 (14.3%) showed a concurrent increase to hRSV. We speculated that common antigens shared by these viruses explain this finding. Since the nucleocapsid (N) proteins of these viruses show the greatest sequence homology, we tested hyperimmune antisera prepared for each virus against baculovirus-expressed recombinant N (recN) proteins for potential cross-reactivity. The antisera were reciprocally reactive with both proteins. To localize common antigenic regions, we first expressed the carboxy domain of the hMPV N protein that was the most highly conserved region within the hRSV N protein. Although reciprocally reactive with antisera by Western blotting, this truncated protein did not react with hMPV IgG-positive human sera by EIA. Using 5 synthetic peptides that spanned the amino-terminal portion of the hMPV N protein, we identified a single peptide that was cross-reactive with human sera positive for either virus. Antiserum prepared for this peptide was reactive with recN proteins of both viruses, indicating that a common immunoreactive site exists in this region.H uman respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) are negative single-stranded, enveloped RNA viruses that are coclassified within the Pneumovirinae subfamily of the Paramyxoviridae. hRSV is the leading cause of severe lower respiratory tract infections in infants and young children and has been associated with community-acquired pneumonia in adults, with the highest mortality rates found among the hospitalized elderly and immunocompromised (1, 2, 3). hMPV was identified first in 2001 by van den Hoogen et al. (4) and subsequently has been shown to have clinical and epidemiological features similar to but distinct from those of hRSV (5, 6). Most hMPV infections occur within the first few years of life, usually later than those for hRSV, and cause 5 to 10% of respiratory infections in children requiring hospitalization, second only to hRSV (7). Both viruses are ubiquitous globally, and recent studies have documented a high prevalence of infection among children in less developed countries (8, 9). Clinical outcomes similar to those for hRSV have been described for hMPV infections in the frail elderly (10).The RNA genomes of hRSV and hMPV are complexed with nucleocapsid (N) protein that together serve as the templates for genome replication and transcription (11). The N protein is the most abundant...

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Seroprevalence of human metapneumovirus (hMPV) in the Canadian province of Saskatchewan analyzed by a recombinant nucleocapsid protein‐based enzyme‐linked immunosorbent assay

Cited by 24 publications

References 21 publications

Human Metapneumovirus: Lessons Learned over the First Decade

Human Metapneumovirus: Lessons Learned over the First Decade

Seroprevalence of human respiratory syncytial virus and human metapneumovirus in healthy population analyzed by recombinant fusion protein-based enzyme linked immunosorbent assay

Serologic Cross-Reactions between Nucleocapsid Proteins of Human Respiratory Syncytial Virus and Human Metapneumovirus

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