Seroprevalence among blood donors is a major public health problem, both in developed and developing countries, in its magnitude. The aim of this study was to determine the seroprevalence of hepatitis B in blood donors. This is a descriptive study carried out in the city of Mbuji-Mayi at the General Hospital of Dipumba in blood donors (family, volunteer and remunerated) recorded from 01/to31/December 2016; the data were collected in a transverse fashion. The following observations were made: During the study period, 1584 blood donors were registered. After analyzing the data, the seroprevalence of hepatitis B in blood donors was 2.2%, 77.8% were male (sex ratio M/F 3.5 and voluntary donors were 50.4%.