Seroprevalence of hepatitis E virus among blood donors in Qatar (2013‐2016)

Nasrallah, Gheyath K.; Absi, Enas S. Al; Ghandour, Rula; Ali, Nadima H.; Taleb, Sara; Hedaya, Laila; Ali, Fatima; Huwaidy, Mariam; Husseini, Abdullatif

doi:10.1111/trf.14116

Cited by 49 publications

(37 citation statements)

References 33 publications

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“…The 3 HEV RNA negative and IgM‐positive donors with their anti‐HEV IgG negative status is a scenario of HEV infection in endemic countries. Similar type of silent acute HEV infection at the time of donation has been reported in studies including a recent one by Nasrallah et al, in a Qatar‐based study, where HEV antibody seroprevalence was 20.8% and had only 18 of 34 IgM‐positive samples who were also IgG positive. This indicated that this profile in the blood donors in the HEV endemic countries is not uncommon.…”

Section: Discussionsupporting

confidence: 78%

Hepatitis E virus seroprevalence among blood donors in Pune, India

Tripathy

Puranik

Sharma

et al. 2018

Journal of Medical Virology

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Background Blood transfusion is a recently reported route of hepatitis E virus (HEV) transmission. It is a bigger concern in regions where large‐scale HEV genotype 1 infections occur causing more severe disease. The present study aims to assess the prevalence and rate of HEV infection in the blood donors of Pune, India. Materials and Methods A total of 2447 healthy blood donors were screened for anti‐HEV IgG and IgM antibodies. Anti‐HEV IgM antibody positives were further subjected to alanine aminotransferase measurement, HEV RNA detection, viral load quantification and phylogenetic analysis. Results Anti‐HEV seroprevalence rate was 17.70%, while IgM prevalence rate was 0.20%. An age dependent increase in IgG seropositive rate was observed. Two of five IgM‐positives tested positive for HEV RNA. The viral load ranged from 3.5 × 104 to 4.6 × 105 copies/mL and belonged to HEV genotype 1. Conclusions HEV prevalence rate of 17.70% in the blood donors of Pune, India, a developing country, goes at par with the developed countries. Current data of 0.20% (5 of 2447) blood donors positive for anti‐HEV IgM and two of them being HEV RNA positive suggest a need for consideration of cost‐effective evaluation towards pooled HEV RNA testing in blood banks.

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Section: Discussionsupporting

confidence: 78%

Hepatitis E virus seroprevalence among blood donors in Pune, India

Tripathy

Puranik

Sharma

et al. 2018

Journal of Medical Virology

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“…In fact, it is very well documented that the correlation between HEV IgM and RNA is not significant, and HEV IgM screening methods have lower sensitivity compared with nucleic acid amplification methods . Nevertheless, the viremia ratio reported in the study conducted in Qatar is considered very high when compared with similar studies conducted in developed countries in North America, Europe, and Australia (Table ).…”

Section: Mode Of Transmissionmentioning

confidence: 84%

“…HEV infection is usually self-limiting, and its mortality rate is generally 0.5% to 4.0%. 3,69 However, in pregnant women, HEV mortality rate Qatar 5854 20.5 Qanti-ELISA 2017 37 increases to 20% to 25% ( Figure 1). 1,70 71 The mechanism by which HEV contributes to these diseases is still under investigation and other possible infections must be ruled out.…”

Section: Mode Of Transmissionmentioning

confidence: 98%

“…Out of 900 tested samples, 39 (4.3%) were positive for HEV IgM, while 168 (18.7%) were positive for HEV IgG . More recently, a large study (n = 5,854) conducted in Qatar estimated HEV seroprevalence among healthy blood donors aged between 25 and 34 years to be approximately 20.7% . Out of the 5,854 samples, 1,198 (20.5%) tested positive for HEV IgG, while 34 (0.58%) tested positive for HEV IgM.…”

Section: Mode Of Transmissionmentioning

confidence: 99%

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Seroprevalence and incidence of hepatitis E virus among blood donors: A review

2017

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Hepatitis E virus (HEV) is an RNA virus with 4 main genotypes. HEV-1 and HEV-2 infect solely humans, while HEV-3 and HEV-4 infect humans and various animals such as pigs, deer, and rabbits. HEV-5 and HEV-6 infect mainly wild boar. Recently, new genotypes, known as HEV-7 and HEV-8, were found to infect camels and humans. HEV is globally distributed into different epidemiological patterns based on socioeconomic factors and ecology. Although HEV is mainly transmitted through the fecal-oral route, it was also recognized as a transfusion-transmitted virus. Transmission through blood donation was documented worldwide with rising annual observations, accounting for more than 2.5% of all transmissions. HEV infection is usually asymptomatic or subclinical in immunocompetent individuals, so it remains questionable whether there is an urgent need to screen for HEV prior to blood transfusion. Moreover, recent studies conducted in the Middle East and North Africa (MENA) region indicate that HEV is highly endemic. Here, we provide a review on HEV epidemiology, transmission, and laboratory diagnosis, giving special emphasis to the newly discovered genotypes, HEV-7 and HEV-8. Furthermore, we underscore the findings of recent HEV seroprevalence and viremia studies among blood donors worldwide. We also shed light on similar studies performed among blood donors in the MENA region.

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“…Even though all of the patients had anti-HEV IgM, larger regions within ORF2 could be amplified in a fraction of them. This inconsistency has been previously observed associated with important differences in the performance of serological and PCR assays [19,20]. In addition, noteworthy differences in the sensitivity and efficiency of detection have been observed among different HEV genome regions [21].…”

Section: Discussionmentioning

confidence: 87%

Hepatitis E Virus Genotype 1 and Hepatitis A Virus Dual Infection in Pediatric Patients with a Low Socioeconomic Status from Mexico

et al. 2018

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Objective: We aimed to detect and characterize hepatitis E virus (HEV) RNA in sera samples from a pediatric population infected with the hepatitis A virus (HAV) exhibiting acute hepatitis and to correlate the infection status with the clinical outcome. Methods: Seventy-five ELISA-positive samples from children containing anti-HAV and anti-HEV IgM were used to amplify and characterize partial regions within HEV ORF2. A statistical comparison of clinical data between HEV IgM-positive/HEV RNA-positive patients and HEV IgM-positive/HEV RNA-negative patients was performed. Results: Thirteen out of 75 IgM-positive samples provided amplification of discrete regions of the HEV genome. Nested RT-PCR-based detection and subsequent sequencing of 5 samples confirmed the identity of HEV genotype 1 (G1), which had not been previously reported in Mexico. Though not significant, a trend towards exacerbated clinical manifestations was found in HEV RNA-positive patients relative to HEV RNA-negative patients. Conclusions: An elevated rate of G1 RNA was detected. Hepatitis E seems to be a neglected disease in Mexico and epidemic strains of HEV are likely to play a role as causative agents of acute hepatitis in highly exposed children. Although HAV is endemic in Mexico, an HEV-RNA detection rate of 17% in co-infected samples shows the need for screening for HEV as a part of future vaccination strategies.

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Seroprevalence of hepatitis E virus among blood donors in Qatar (2013‐2016)

Cited by 49 publications

References 33 publications

Hepatitis E virus seroprevalence among blood donors in Pune, India

Hepatitis E virus seroprevalence among blood donors in Pune, India

Seroprevalence and incidence of hepatitis E virus among blood donors: A review

Hepatitis E Virus Genotype 1 and Hepatitis A Virus Dual Infection in Pediatric Patients with a Low Socioeconomic Status from Mexico

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