Seroprevalence of Hepatitis E Virus in Animal Workers in Nonporcine Consumption Region of Turkey

Aydın, Hakan; Uyanık, Muhammet Hamidullah; Karameşe, Murat; Timurkan, Mehmet Ozkan

doi:10.2217/fvl-2016-0075

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…norvegicus populations are significant players in the ecology of zoonotic diseases and carry zoonotic pathogens in urban settings [14]. They are particularly well-adapted to residing close to places where people live, and they may result in significant economic losses by damaging materials, consuming and defecating on food sources, and transmitting [15]. Implementing control measures will be much easier if the epidemiology and ecology of the pathogenic agent are better understood.…”

Section: Discussionmentioning

confidence: 99%

Intestinal Colonization by Campylobacter jejuni, Clostridium difficile, and Clostridium perfringens among Commensal Rattus norvegicus in the Urban Areas of Tehran, Iran

Azimi,

Nasrollahian,

Sabour

et al. 2024

BioMed Research International

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Background. Rattus norvegicus (R. norvegicus) population plays a significant role in the spread of numerous diseases in urban environments. The present study is aimed at investigating the presence of Campylobacter jejuni (C. jejuni), C. coli, Clostridium difficile (C. difficile), C. difficile toxigenic, and C. perfringens in R. norvegicus captured from urban areas of Tehran, Iran. Methods. From October 2021 to October 2022, 100 urban rats were trapped in 5 different districts of Tehran, Iran. The genomic DNA was extracted from fecal samples, and the presence of C. jejuni, C. coli, C. perfringens, and C. difficile species was evaluated using PCR assay. Moreover, PCR was used to assess the toxicity of C. difficile isolates. Results. Overall, 30% (n=30/100) of fecal samples were positive for zoonotic pathogens. Based on the PCR on hippuricase (hipO), glycine (gly), CIDIF, and phospholipase C (plc) genes, C. perfringens and C. difficile were isolated from 18.2% (n=14/77) and 5.2% (n=4/77) of male rats. The highest frequency of C. perfringens and C. jejuni was 25% (n=5/20) related to the south of Tehran. Toxigenic C. difficile was not detected in all regions. Conclusion. According to the findings, rats are the main reservoirs for diseases. Therefore, rodent control coupled with the implementation of surveillance systems should be prioritized for urban health.

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Section: Discussionmentioning

confidence: 99%

Intestinal Colonization by Campylobacter jejuni, Clostridium difficile, and Clostridium perfringens among Commensal Rattus norvegicus in the Urban Areas of Tehran, Iran

Azimi,

Nasrollahian,

Sabour

et al. 2024

BioMed Research International

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“…[2,4] In Turkey, similar HEV seroprevalence data have been reported in animal and agricultural workers (35.9% and 34.8%, respectively). [32,33] Because detailed queries regarding the jobs of the patients were not performed, the relationship between profession, and HEV seropositivity could not be investigated. However, no difference was found between patients living in urban and rural areas in terms of anti-HEV IgG antibody positivity.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis E virus IgG seroprevalence in liver transplant patients: A retrospective single-center experience

Çelik¹,

Şenkaya²,

Ünal³

et al. 2021

Hepatology Forum

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Background and Aim: Hepatitis E virus (HEV) may cause chronic liver disease in solid organ transplant recipients. We determined HEV seroprevalence and associated factors in liver transplant recipients. Materials and Methods: Patients followed at the outpatient clinic of liver transplantation between January 2019 and January 2020 were screened retrospectively for HEV serology (HEV immunoglobulin M [IgM] and HEV immunoglobulin G [IgG]). Results: Of the 150 patients (male/female, 104/46; age, 55.4±13.2 years), anti-HEV IgG was positive in 31 (20.7%), and anti-HEV IgM was negative in all. The mean time after liver transplantation (72 [48%] deceased and 78 [52%] living donors) was 81±78.5 months. Drinking water consisted of carboy and tap water in 88 (58.7%) and 62 patients (41.3%), respectively.Of the patients, 120 (80%) and 30 (20%) lived in urban and rural areas, respectively. On comparison, the difference between positive and negative anti-HEV IgG groups in terms of age, place of birth, water supply, and donor type was statistically significant (p=0.007, p=0.000, p=0.034, and p=0.049, respectively). Conclusion:HEV seroprevalence was more frequent in liver transplant recipients compared with the normal population. Older age, water supply, and place of birth were risk factors for HEV seroprevalence.

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“…PCR analysis was performed to investigate rotavirus (RV), hepatitis E virus (HEV), hepatitis A virus (HAV) , and Norovirus (NoV), that could be transmitted by the fecal-oral route. Primer sets used for each virus are shown in TABLE III [9,29,46,60]. Each of the REVS samples were put 2 g in the tube and diluted with 1.5 mL of phosphate-buffered saline (PBS).…”

Section: Viral Ribonucleic Acid (Rna) Detectionmentioning

confidence: 99%

“…The amplicons obtained from PCR were visualized by gel-electrophoresis and positive results on the gel were recorded for each virus. Positive control samples were viruses that have been previously confirmed by sequence analysis [5,9].…”

Section: Viral Ribonucleic Acid (Rna) Detectionmentioning

confidence: 99%

Microbiological quality of ready–to–eat vegetables salads served at meat restaurants under the COVID-19 in Turkey

2022

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In Turkish cuisine, ready–to–eat vegetable salads (REVS) served with pide/lahmacun, kebab types, and tantuni from animal source in meat restaurants were evaluated since they have the potential to carry risks in terms of Public Health. The microbiological properties of REVS were investigated using agar plate method. Antimicrobial resistance of foodborne pathogens including Escherichia coli and Staphylococcus aureus was tested using Kirby–Bauer disc diffusion method. Moreover, the presence of important enteric viruses was detected by Polymerase Chain Reaction (PCR). The number of total aerobic bacteria, coliform bacteria, yeast and molds and, Staphylococcus and Micrococcus spp. ranged from less than 1 to 6.40, 1 to 6.26, less than 1–5.82 and less than 1–5.66 log10 colony forming units·grams-1 (CFU·g–1) in REVS samples, respectively. None of the REVS tested in this study contained Salmonella spp., whereas E. coli and S. aureus were isolated in 38.1% (16/42) and 2.4% (1/42), respectively. S. aureus was resistant to gentamicin, kanamycin, aztreonam, and ciprofloxacin in the disc diffusion assay, however, it was not harboring the mecA gene. E. coli strains (n=16) were resistant (100%) to aminoglycoside antibiotics and 35.7% (6/16) of the isolates were extended spectrum beta lactamase (ESBL) producing. blaTEM and blaCTXM8/25 were detected in two isolates, whereas one isolate carried blaCTXM–1 and blaTEM together by PCR. Of the REVS, two were evaluated as positive for rotavirus (4.8%), six for hepatitis A (14%), and hepatitis E virus (14%). These results indicate the high microorganism load, presence of ESBL E. coli, and viral enteric pathogens in REVS, hence it is important to perform routine hygiene practices.

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Seroprevalence of Hepatitis E Virus in Animal Workers in Nonporcine Consumption Region of Turkey

Cited by 8 publications

References 26 publications

Intestinal Colonization by Campylobacter jejuni, Clostridium difficile, and Clostridium perfringens among Commensal Rattus norvegicus in the Urban Areas of Tehran, Iran

Intestinal Colonization by Campylobacter jejuni, Clostridium difficile, and Clostridium perfringens among Commensal Rattus norvegicus in the Urban Areas of Tehran, Iran

Hepatitis E virus IgG seroprevalence in liver transplant patients: A retrospective single-center experience

Microbiological quality of ready–to–eat vegetables salads served at meat restaurants under the COVID-19 in Turkey

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