Seroprevalence of fourteen human polyomaviruses determined in blood donors

Kamminga, Sergio; Meijden, Els Pz van der; Feltkamp, Mariet C.W.; Zaaijer, Hans

doi:10.1101/357350

Cited by 21 publications

(6 citation statements)

References 38 publications

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“…Our results on prevalence and persistence of MCPyV, HPyV6, and HPyV7 are in concordance with serological studies in healthy individuals that found antibodies against MCPyV, HPyV6, and HPyV7 capsid proteins in 81.9, 83.8, and 71.7% of healthy blood donors, respectively (Kamminga et al, 2018). TSPyV-and HPyV9-specific antibodies were detected in 80-90% and >30-70%, respectively, and primary infection seems to occur during early childhood and adolescence (Foulongne et 2012;Nicol et al, 2013;Kamminga et al, 2018). Hence, it is possible that the skin is not the only tissue infected by these viruses.…”

Section: Discussionsupporting

confidence: 91%

“…This notion is supported by several studies, in which TSPyV and HPyV9 were detected sporadically in tonsil tissue, faeces, respiratory samples, urine or peripheral blood, mainly in immunosuppressed persons (Rockett et al, 2013;Herberhold et al, 2017;Kourieh et al, 2018;Borgogna et al, 2019;Kamminga et al, 2019). In our study, short-term persistence rates of HPyV10 were low and long-term persistence did not occur, although HPyV10 reaches a seroprevalence rate of nearly 100% in adults (Kamminga et al, 2018). This indicates that the skin is only transiently infected and does not represent a permanent viral reservoir of HPyV10.…”

Section: Discussionsupporting

confidence: 86%

“…HPyV10/MWPyV has been found in respiratory specimens, in 18.4% of tonsil cancer samples and in 5-7% of stool specimens from children and adults (Rockett et al, 2013;Herberhold et al, 2017;Pinheiro et al, 2020). The relatively high overall seroprevalence of STLPyV (64.8%) compared to the low DNA prevalence and persistence rate detected in our study also suggests sites of infection other than the skin, particularly the gut (Lim et al, 2013;Kamminga et al, 2018).…”

Section: Discussionsupporting

confidence: 44%

Section: Introductionmentioning

confidence: 99%

“…Several studies have reported high seroprevalence (60–100%) for the majority of HPyVs in adults ( Nicol et al, 2013 ; Van Der Meijden et al, 2013 ; Gossai et al, 2016 ; Kamminga et al, 2018 ). Lower seroprevalence rates (around 5%) were found for the recently identified HPyV12, NJPyV-2013, and LIPyV ( Kamminga et al, 2018 ). Although serological evidence indicates that most adults have been immunologically exposed to the majority of HPyVs, the natural history of HPyV infections in healthy individuals has not yet been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Natural History of Cutaneous Human Polyomavirus Infection in Healthy Individuals

et al. 2021

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Several human polyomaviruses (HPyVs) were recently discovered. Merkel cell polyomavirus (MCPyV) induces Merkel cell carcinoma. HPyV6, HPyV7, and TSPyV have been associated with rare skin lesions in immunosuppressed patients. HPyV9, HPyV10, and Saint Louis Polyomavirus (STLPyV) have not been convincingly associated with any disease. The aim of this prospective study was to evaluate the cutaneous prevalence, persistence and viral load of HPyVs in healthy individuals. Eight hundred seventy forehead and hand swabs were collected from 109 volunteers 4–6 weeks apart (collection period-1). Fifty-nine participants were available for follow-up a decade later (collection period-2). HPyV-DNA prevalence and viral loads of MCPyV, HPyV6, HPyV7, TSPyV, HPyV9, HPyV10, and STLPyV were determined by virus-specific real-time PCRs. Risk factors for HPyV prevalence, short- and long-term persistence were explored by logistic regression analyses. Baseline prevalence rates were similar for forehead and hand: MCPyV 67.9/67.0%, HPyV6 31.2/25.7%, HPyV7 13.8/11.0%, HPyV10 11.9/15.6%, STLPyV 7.3/8.3%, TSPyV 0.9/0.9%, and HPyV9 0.9/0.9%. Short-term persistence in period-1 was found in 59.6% (MCPyV), 23.9% (HPyV6), 10.1% (HPyV7), 6.4% (HPyV10), 5.5% (STLPyV), and 0% (TSPyV and HPyV9) on the forehead, with similar values for the hand. Long-term persistence for 9–12 years occurred only for MCPyV (forehead/hand 39.0%/44.1% of volunteers), HPyV6 (16.9%/11.9%), and HPyV7 (3.4%/5.1%). Individuals with short-term persistence had significantly higher viral loads at baseline compared to those with transient DNA-positivity (p < 0.001 for MCPyV, HPyV6, HPyV7, and HPyV10, respectively). This was also true for median viral loads in period-1 of MCPyV, HPyV6, and HPyV7 of volunteers with long-term persistence. Multiplicity (two or more different HPyVs) was a risk factor for prevalence and persistence for most HPyVs. Further risk factors were older age for HPyV6 and male sex for MCPyV on the forehead. Smoking was not a risk factor. In contrast to MCPyV, HPyV6, HPyV7, and rarely STLPyV, polyomaviruses TSPyV, HPyV9, and HPyV10 do not seem to be long-term constituents of the human skin virome of healthy individuals. Furthermore, this study showed that higher viral loads are associated with both short- and long-term persistence of HPyVs on the skin. HPyV multiplicity is a risk factor for prevalence, short-term and/or long-term persistence of MCPyV, HPyV6, HPyV7, and HPyV10.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 44%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Natural History of Cutaneous Human Polyomavirus Infection in Healthy Individuals

et al. 2021

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Wastewater-based epidemiology applied at the building-level reveals distinct virome profiles based on the age of the contributing individuals

Mejías-Molina,

Pico-Tomàs,

Martínez-Puchol

et al. 2024

Hum Genomics

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Background Human viruses released into the environment can be detected and characterized in wastewater. The study of wastewater virome offers a consolidated perspective on the circulation of viruses within a population. Because the occurrence and severity of viral infections can vary across a person’s lifetime, studying the virome in wastewater samples contributed by various demographic segments can provide valuable insights into the prevalence of viral infections within these segments. In our study, targeted enrichment sequencing was employed to characterize the human virome in wastewater at a building-level scale. This was accomplished through passive sampling of wastewater in schools, university settings, and nursing homes in two cities in Catalonia. Additionally, sewage from a large urban wastewater treatment plant was analysed to serve as a reference for examining the collective excreted human virome. Results The virome obtained from influent wastewater treatment plant samples showcased the combined viral presence from individuals of varying ages, with astroviruses and human bocaviruses being the most prevalent, followed by human adenoviruses, polyomaviruses, and papillomaviruses. Significant variations in the viral profiles were observed among the different types of buildings studied. Mamastrovirus 1 was predominant in school samples, salivirus and human polyomaviruses JC and BK in the university settings while nursing homes showed a more balanced distribution of viral families presenting papillomavirus and picornaviruses and, interestingly, some viruses linked to immunosuppression. Conclusions This study shows the utility of building-level wastewater-based epidemiology as an effective tool for monitoring the presence of viruses circulating within specific age groups. It provides valuable insights for public health monitoring and epidemiological studies.

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Merkel Cell Polyomavirus in the Context of Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders

Passerini,

Babini,

Merenda

et al. 2024

Biomedicines

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Despite recent advances in prevention, detection and treatment, oral squamous cell carcinoma (OSCC) remains a global health concern, strongly associated with environmental and lifestyle risk factors and infection with oncogenic viruses. Merkel Cell Polyomavirus (MCPyV), well known to be the causative agent of Merkel Cell Carcinoma (MCC) has been found in OSCC, suggesting its potential role as a co-factor in the development of oral cavity cancers. To improve our understanding about MCPyV in oral cavities, the detection and analysis of MCPyV DNA, transcripts and miRNA were performed on OSCCs and oral potentially malignant disorders (OPMDs). In addition, the cellular miR-375, known to be deregulated in tumors, was examined. MCPyV DNA was found in 3 out of 11 OSCC and 4 out of 12 OPMD samples, with a viral mean value of 1.49 × 102 copies/mL. Viral integration was not observed and LTAg and VP1 transcripts were detected. Viral miRNAs were not detected whereas the cellular miR-375 was found over expressed in all MCPyV positive oral specimens. Our results reported evidence of MCPyV replication in both OSCC and OPMD suggesting the oral cavity as a site of replicative MCPyV infection, therefore underscoring an active role of this virus in the occurrence of oral lesions.

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Seroprevalence of fourteen human polyomaviruses determined in blood donors

Abstract: 10The polyomavirus family currently includes thirteen human polyomavirus (HPyV)

Cited by 21 publications

References 38 publications

Natural History of Cutaneous Human Polyomavirus Infection in Healthy Individuals

Natural History of Cutaneous Human Polyomavirus Infection in Healthy Individuals

Wastewater-based epidemiology applied at the building-level reveals distinct virome profiles based on the age of the contributing individuals

Merkel Cell Polyomavirus in the Context of Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders

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