Seroprevalence of Canine Monocytic Ehrlichiosis in Hunting Dogs in the Autonomous Province of Vojvodina, Serbia

Potkonjak, Aleksandar; Savić, Sara; Kosić, Ljubica Spasojević; Vračar, Vuk; Kudus, Radmila; Suvajdžić, Ljiljana; Radišić, Nenad

doi:10.46784/e-avm.v7i1.120

Cited by 6 publications

(7 citation statements)

References 12 publications

(9 reference statements)

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“…Previous research has confirmed that R. sanguineus most often infests dogs in our country as well (Bogunović et al., 2018 ; Potkonjak et al., 2016 ). Even though previous seroepidemiological studies conducted in Serbia have confirmed the exposure of dogs to E. canis (Bogićević et al., 2017 ; Potkonjak et al., 2013 ), clinical cases of CME or molecular confirmation of the causative agent have not been reported before (Kovačević Filipović et al., 2018 ). Also, the presence of E. canis in R. sanguineus ticks collected from dogs in Serbia has not been confirmed so far (Potkonjak et al., 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia

Sukara

Andrić

et al. 2022

Veterinary Medicine & Sci

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Background The epidemiological status concerning many canine tick‐borne diseases (TBDs) in Serbia is still insufficiently known. Objectives Our study aimed to investigate the presence of tick‐borne pathogens of the family Anaplasmataceae and Hepatozoon spp., as a cause of illnesses accompanied by clinical signs that can occur in dogs with anaplasmosis, ehrlichiosis and hepatozoonosis. Methods Dogs are included in the study based on the presence of a minimum of three clinical and/or pathological findings that could be associated with anaplasmosis, ehrlichiosis and hepatozoonosis. During the study (April–October 2018), 11 dogs met the conditions to be included in the survey. Identification of the causative agent in the blood of diseased dogs was performed by conventional PCR followed by sequencing. Results The presence of the pathogens was confirmed in three animals (3/11, 27.3%). The presence of Ehrlichia canis was confirmed in 3‐month‐old female Rottweiler puppy, an 8‐year old Miniature Schnauzer female was positive for Hepatozoon canis infection, while 4‐year‐old mixed breed male dog was co‐infected with both mentioned pathogens. These are the first cases of autochthonous infection with E. canis and H. canis in dogs from Serbia confirmed by molecular methods. Conclusions The results of our study indicate the importance of molecular methods to establish a reliable diagnosis of TBDs. Also, the confirmed presence of causative agents of canine monocytic ehrlichiosis and hepatozoonosis in Serbia appeals to veterinary practitioners that it is necessary to exclude the presence of those diseases in suspicious dogs.

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Section: Discussionmentioning

confidence: 99%

Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia

Sukara

Andrić

et al. 2022

Veterinary Medicine & Sci

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“…In this study, A. phagocytophilum infection was confirmed in the family’'s dogs. Domestic dogs are considered as potential reservoirs for this pathogen in Serbia [ 34 ]. A. phagocytophilum is the causative agent of granulocytic anaplasmosis in humans and animals.…”

Section: Discussionmentioning

confidence: 99%

A One Health approach to study the circulation of tick-borne pathogens: A preliminary study

et al. 2021

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Tick-borne pathogens (TBPs) have complex life cycles involving tick vectors and vertebrate hosts. However, there is limited empirical evidence on the zoonotic circulation of TBPs. In this study, we used a One Health approach to study the possible circulation of TBPs in ticks, animals and humans within a rural household in the foothills of the Fruška Gora mountain, northern Serbia. The presence of TBP DNA was assessed using microfluidic PCR (25 bacterial species, 7 parasite species, 5 bacterial genera, 3 parasite genera) in animal, human and tick samples and the presence of tick-borne encephalitis virus (TBEV) RNA was screened for using RT-qPCR on tick samples. In addition, Lyme borreliosis serology was assessed in patients sera. Rhipicephalus sanguineus and Ixodes ricinus ticks were identified on dogs and Haemaphysalis punctata was identified on house walls. Rickettsia helvetica was the most common pathogen detected in pooled R. sanguineus and I. ricinus tick samples, followed by Hepatozoon canis . None of the H. punctata tick samples tested positive for the presence of TBPs. Anaplasma phagocytophilum and Rickettsia monacensis were the most frequent pathogens detected in dogs, followed by Rickettsia felis , whereas Anaplasma bovis was the only pathogen found in one of the goats tested. None of the human blood samples collected from family members tested positive for the presence of TBPs. Although microfluidic PCR did not detect Borrelia sp. in any of the tested tick or blood samples, a family member with a history of Lyme disease was seropositive for Borrelia burgdorferi sensu lato (s.l.). We conclude that, despite the presence of TBPs in tick and vertebrate reservoirs, there is no evidence of infection with TBPs across various components of the epidemiological chain in a rural Fruška Gora household.

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“…Serological studies conducted in dogs in Serbia confirmed they are exposed to A. phagocytophilum. Using the immunofluorescence antibody test (IFAT), a seroprevalence of 15.5% was recorded in analyzed dogs from Vojvodina (Potkonjak et al, 2015), while dogs from Belgrade had a seroprevalence of 26.1% (Kovačević Filipović et al, 2018). Although previous studies indicate that dogs are exposed to the causative agent of canine anaplasmosis, there have been no confirmed cases of this disease in Serbia to date.…”

Section: Role Of Jackals and Foxes In The Epidemiology Of Tick-borne mentioning

confidence: 98%

Wild canids as hosts for ticks and tick-borne zoonotic pathogens in Serbia

et al. 2020

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Wild canids are recognized as important reservoir hosts for several vector-borne pathogens and are a good sentinel species for epidemiological surveillance. Identifying the reservoir hosts for particular zoonotic pathogens in a specific area enables the implementation of adequate measures in order to prevent the occurrence of zoonotic diseases in humans and domestic animals. The present review aims to summarize the role of wild canids in the epidemiology of tick-borne zoonoses in Serbia based on previously published papers and to discuss the available data from an epidemiological point of view. The results of previously published studies indicate that wild canids are potential reservoir hosts for several zoonotic tick-borne pathogens in Serbia, and hosts for several tick species which are proven competent vectors. Based on molecular studies conducted so far, the presence of Anaplasma phagocytophilum, was confirmed in jackals while the presence of Candidatus Neoehrlichia micurensis (FU98), Borrelia burgdorferi sensu stricto, Borrelia garinii, and Borrelia lusitaniae was proven by PCR in analyzed red foxes. There is a need for continuous epidemiological monitoring of diseases caused by pathogens previously confirmed in wild canids. Also, further research is needed to reveal the role of other wild and domestic animals in the epidemiology of tick-borne zoonoses in Serbia.

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Seroprevalence of Canine Monocytic Ehrlichiosis in Hunting Dogs in the Autonomous Province of Vojvodina, Serbia

Cited by 6 publications

References 12 publications

Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia

Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia

A One Health approach to study the circulation of tick-borne pathogens: A preliminary study

Wild canids as hosts for ticks and tick-borne zoonotic pathogens in Serbia

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