Seroprevalence of Brucella antibodies in camels in Katsina State, Nigeria

Salisu, U.S.; Kudi, Caleb Ayuba; Bale, J. O. O.; Babashani, M; Kaltungo, B. Y.; Saidu, S. N. A.; Asambe, A.; Baba, A.Y.

doi:10.1007/s11250-017-1297-5

Cited by 11 publications

(6 citation statements)

References 18 publications

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“…Since then, it has been testified by all camel rearing countries like Sudan, Ethiopia, Somalia, Kenya, Nigeria, Jordan and Egypt but not Australia [3]. There is no separate specific species of Brucella that displays a preference for camels as they can be infected by those that have already been shown to be prevalent in bovines, ovines and caprines [4][5][6]. The clinical picture of brucellosis in camels can vary from asymptomatic to abortion, retention of fetal membranes, weak offspring, impaired fertility and delayed sexual maturity in females and orchitis accompanied by lameness in males [2,4,7].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt

et al. 2020

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Brucellosis is one of the most important worldwide zoonoses of many countries including Egypt. Camel brucellosis has not gained much attention in Egypt yet. This study is focused on the three governorates with the highest camel populations and the largest camel markets in the country to determine the disease seroprevalence and identify the Brucella species in local camel holdings. In total, 381 serum samples were collected from male and female camels from Giza, Aswan, and Al-Bahr Al-Ahmar (the Red Sea) governorates. Samples were serologically examined using the Rose–Bengal plate test (RBPT), indirect ELISA (i-ELISA), competitive ELISA (c-ELISA) and complement fixation test (CFT). Brucella antibodies were detected in 59 (15.5%), 87 (22.8%), 77 (20.2%) and 118 (31.0%) of sera by RBPT, i-ELISA, c-ELISA and CFT, respectively. Using real-time PCR, Brucella DNA was amplified in 32 (8.4%) seropositive samples including Brucella abortus (25/32), Brucella suis (5/32) and Brucella melitensis (2/32), defining a complex epidemiological status. To the best of our knowledge, this is the first study reporting Brucella suis DNA in camel serum. The risk-associated factors including age, sex, breed and geographical distribution were statistically analyzed, showing non-significant association with seroprevalence. The results of this study will raise awareness for camel brucellosis and help develop effective control strategies.

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Section: Introductionmentioning

confidence: 99%

Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt

et al. 2020

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“…The primary clinical signs of infection are abortion in females, and orchitis and epididymitis with frequent sterility in males, because of the localization of brucella within the male and female reproductive organs. Large numbers of brucellae can be excreted in fetal fluids and mammary secretions [28,29]. This research work was carried out on A total of 203 (serum -45, placental tissue -40, blood -25, placental fluid -13, milk -50, uterine swab -30) samples of dromedary camels suspected to be or had a history of brucellosis from Qatar.…”

Section: Resultsmentioning

confidence: 99%

A Novel Evidence of Immunological and Molecular Detection of Brucella Species in Camels

Manivannan¹,

Ramasamy²,

Ahmed³

2021

JPRI

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Objectives: Brucellosis is a zoonosis with severe complications for both humans and animals. In this work, we intended to examine the Brucella infection in dromedary camels in Qatar by using different analysis. Materials and Methods: A total of 203 samples of dromedary camels were randomly collected from the nearby farms in Qatar. Real-time PCR for the genus specific Brucella cell surface salt extractable bcsp31 kDa protein gene were performed on DNA extracted from camel samples. Rose Bengal and rivenol tests were performed to detect the Brucella species. The milk samples were collected from the camels and utilized for the milk ring test. Results: The outcomes of RT-PCR analysis illustrate the presence of Brucella spp. in 170 samples (83.74%) out of 203 samples. The findings of immunological assays also proved the presence of Brucella spp. such as Rose Bengal (67.14%), ELISA (71.42%), and precipitation assay (65.71) in both serum and blood samples of the dromedary camels, which were collected from the Qatar. Conclusions: In conclusion, it was clear that the incidence of the brucellosis in camels is significantly rising in Qatar region and there is a need to control the spread of the disease from camels to camels as well as from camels to humans.

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“…A previous report discusses 15 camels that were set up to be infected with Brucella in Jordan. 34 Whereas, in another study conducted in Sudan revealed that 40 tested samples were positive for Brucella when 2,000 camel serum and milk samples were examined. 35 Bacterial infections are transmitted to humans from other sources where antibiotics are used for various purposes and should cause emerging resistant strains.…”

Section: Discussionmentioning

confidence: 97%

Identification of Brucella melitensis from camel’s blood by vitek2 and real time polymerase chain reaction

Manivannan,

Ramasamy,

Sundaresan

et al. 2024

Eur J Clin Exp Med

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Introduction and aim. Brucellosis is a zoonotic disease. Experimental clinical and laboratory diagnosis is still facing problems in identifying the organism. The present study will diagnose a Brucella infection in camel blood in Qatar using serological assays. Isolation and identification were performed on a camel blood sample. Brucella in bacterial isolates was determined by real-time polymerase chain reaction (RT-PCR) as a gold standard test. Material and methods. A total of 220 samples, 200 random serum samples, and 20 EDTA blood samples were selected among the above-mentioned random samples, and 20 serum samples from camel handlers were collected from Al Shahaniya prov ince, Qatar. The Rose Bengal test (RBT), buffered antigen plate agglutination test (BAPAT), and enzyme linked immunosorbent assay (cELISA) for the monoclonal antibody in serum samples were performed using commercially available kits. For the molecular detection of Brucella, conventional PCR and real-time PCR (GPS kit) were used for the genus-specific insertion sequence IS711. Brucella melitensis (MICROBOSS Hightech GmbH kit) was used to identify subspecies. Results. The results identified by vitek2 compact (30%) showed B. melitensis in 6 samples out of 20 isolates. Both conventional (66.67%) and RT-PCR (83.33%) analyses supported this, demonstrating the presence of Brucella. These tests also showed that Brucella species were present in Rose Bengal 182/200 (91%), BAPAT 182/200 (91%), and cELISA (90%) 180/200 in camel serum. Conclusion. To conclude, the prevalence of brucellosis in dromedary camels is higher in this region, and as a matter of urgency, measures should be taken to control the disease.

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Seroprevalence of Brucella antibodies in camels in Katsina State, Nigeria

Cited by 11 publications

References 18 publications

Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt

Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt

A Novel Evidence of Immunological and Molecular Detection of Brucella Species in Camels

Identification of Brucella melitensis from camel’s blood by vitek2 and real time polymerase chain reaction

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