Seroprevalence of bovine leukemia virus in cattle, buffalo, and camel in Egypt

Selim, Abdelfattah; Marawan, Marawan A.; Ali, Abdel-Fattah; Manaa, Eman; AbouelGhaut, Hassab Allah

doi:10.1007/s11250-019-02105-8

Cited by 46 publications

(35 citation statements)

References 13 publications

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“…The total percentage of BLV prevalence determined in our study was 21.5%. This result is in agreement with the results of the recent seroprevalence study reporting BLV infection rate to be 20.8% among Egyptian cattle ( 28 ) and similar to the previously reported BLV infection prevalence rate of 15.83% ( 27 ). Likewise, BLV infection rate reported in some of the neighboring countries include 20.2% in Saudi Arabia ( 42 ) 7.75% in Iraq ( 44 ), despite being high (48.3% in herd level) in Turkey ( 39 ).…”

Section: Discussionsupporting

confidence: 93%

“…In 2012, a seroprevalence study for BLV infection among dairy cattle in Egypt was conducted, which reported that 15.83% of the tested dairy cattle were BLV-infected ( 27 ). Recently, 20.8% BLV seropositivity was reported during the survey for BLV infection among Egyptian cattle ( 28 ). These findings indicate that BLV still infects Egyptian cattle.…”

Section: Introductionmentioning

confidence: 99%

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Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle

Hamada

Metwally

Polat³

et al. 2020

Front. Vet. Sci.

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Bovine leukemia virus (BLV) causes enzootic bovine leukosis (EBL), the most common neoplastic disease in cattle worldwide. The first EBL outbreak in Egypt was reported in 1997. To date, there are few studies regarding BLV diagnosis using only serological detection and no studies investigating the distribution of BLV provirus, which is the retroviral genome integrated into the host genome, in Egypt. The genetic characteristics of Egyptian BLV strains are also unknown. Therefore, we aimed to detect BLV provirus and determine BLV genetic variability among dairy cattle in Egypt. We collected 270 blood samples of dairy cattle from 24 farms located in five provinces in Egypt. Out of the 270 samples, 58 (21.5%) were positive for BLV provirus. Phylogenetic analysis based on 18 420-bp selected sequences out of 50 isolates of the BLV env -gp51 gene demonstrated that Egyptian BLV isolates were clustered into genotype-1 and-4, among 11 genotypes detected worldwide. Furthermore, phylogenetic analysis and alignment of the 501-bp sequence of the env- gp51 gene revealed that at least six genetically different strains are present in Egypt. Genotype-1 isolates comprised four different strains (G1-a, G1-b, G1-c, and G1-d) and genotype-4 isolates included two different strains (G4-x and G4-y). Moreover, in one farm with 100% infection rate, we identified three isolates of G1-a strain, 35 isolates of G4-x strain, and two isolates of G4-y strain. Overall, this study provides the new report on molecular prevalence of BLV in Egypt and records the coexistence of BLV genotype-1 and-4 in Egyptian cattle.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle

Hamada

Metwally

Polat³

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

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“…In 2012, one group reported an infection rate of 15.83% among cattle, using the agar gel immunodiffusion method and ELISA to detect antibodies against BLV in the blood and milk of foreign breeds of cattle in the Kafr El Sheikh, Alexandria and Menofia governorates [20]. Furthermore, in 2018, a study for screening antibodies against BLV by ELISA revealed an infection rate 20.8% in Kafr El Sheikh, Qalyubia and Menofia [38]. The higher infection rate in this study may indicate that the prevalence of BLV in Egypt has increased in the last few years.…”

Section: Discussionmentioning

confidence: 99%

“…The higher prevalence of BLV in beef cattle compared to dairy cattle may be attributed to poor management and the absence of control programs in beef cattle [38]. Alternatively, the breed of cattle investigated may have influenced the results, as breed has an important role in susceptibility and resistance to BLV infection, due to a variety of genetic and immunological factors [21,40,42].…”

Section: Discussionmentioning

confidence: 99%

“…The World Organization for Animal Health (OIE) considers that Egypt has been free from BLV since 1997 [32]. However, a number of serological studies have detected BLV infections among Egyptian cattle in different regions such as, Kafr El Sheikh, Alexandria and Menofia; with infection rate of 15.83% [20], and in Kafr El Sheikh, Qalyubia and Menofia was 20.8% in Egyptian dairy cows [38]. Our recent study investigating the distribution of the BLV provirus, showed that BLV infections were prevalent in 21.5% (58 out of 270 head) of Egyptian dairy cattle in five provinces located in the northern, central, and southern parts of Egypt [11].…”

Section: Introductionmentioning

confidence: 99%

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Detection and molecular characterization of bovine leukemia virus in beef cattle presented for slaughter in Egypt

Metwally

Hamada

Ali

et al. 2020

The Journal of Veterinary Medical Science

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Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, the most common neoplastic disease of cattle worldwide and a serious problem for the cattle industry. Previous studies have shown the molecular prevalence of BLV and the coexistence of BLV genotype-1 and-4 in Egyptian dairy cattle; however, the molecular characteristics of BLV in Egyptian beef cattle are unknown. Therefore, we collected blood samples of 168 beef cattle from slaughterhouses in three governorates in Egypt. Based on BLV-CoCoMo-qPCR-2 targeting long terminal repeats and nested PCR targeting the env-gp51 gene, the BLV provirus infection rates were found to be 47/168 (28.0%) and 42/168 (25.0%), respectively. Phylogenetic analysis based on 501 bp of the BLV env-gp51 gene from 42 BLV isolates revealed that at least six distinctive strains (b, e, f, g, x, and z) were prevalent in cattle across the examined regions. Furthermore, phylogenetic analysis of the 420 bp sequence of the BLV env-gp51 region of the six strains against 11 known genotypes showed that the strains b, e, f, and g were clustered into genotype-1, and strains x and z were clustered into genotype-4. Our results also indicated that strains b and x exist in both dairy and beef cattle in Egypt. The present study is the first to detect and genotype BLV among beef cattle in Egypt.

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Genetic analysis of the pX region of bovine leukemia virus genotype 1 in Holstein Friesian cattle with different stages of infection

et al. 2021

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Seroprevalence of bovine leukemia virus in cattle, buffalo, and camel in Egypt

Cited by 46 publications

References 13 publications

Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle

Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle

Detection and molecular characterization of bovine leukemia virus in beef cattle presented for slaughter in Egypt

Genetic analysis of the pX region of bovine leukemia virus genotype 1 in Holstein Friesian cattle with different stages of infection

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