Seroprevalence and risk factors of Neospora spp. in donkeys from Southern Italy

Machačová, Tereza; Bártová, Eva; Loria, Antonio Di; Sedlák, Kamil; Guccione, Jacopo; Fulgione, Domenico; Veneziano, Vincenzo

doi:10.1016/j.vetpar.2013.08.013

Cited by 24 publications

(21 citation statements)

References 13 publications

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“…in donkeys was 11.8% using a competitive-ELISA (MACHACOVÁ et al, 2013) and in Colombia, 11 of the 56 examined donkeys presented antibodies against Neospora spp. measured by Dot-ELISA (BLANCO et al, 2014).…”

Section: Resultsmentioning

confidence: 96%

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Prevalence of antibodies against Neospora spp. and Sarcocystis neurona in donkeys from northeastern Brazil

Gennari

Pena

Lindsay

et al. 2016

Rev. Bras. Parasitol. Vet.

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Sarcocystis neurona and Neospora hughesi are coccidian protozoa that can cause neurological illness in horses in America. In this study we report seroprevalence of Neospora spp. andS. neurona in sera of 333 donkeys from the northeastern region of Brazil. Antibodies to Neospora spp. were detected in 2% (7 donkeys) of 333 sera tested by the indirect fluorescent antibody test (IFAT) with a cut-off dilution of 1:40. Antibodies to S. neurona were found in 3% (10 donkeys) of the samples tested by IFAT (cut-off ≥50) and 21% (69 donkeys) by the direct agglutination test (SAT ≥50). The SAT and IFAT results for S. neurona showed a poor concordance (value of Kappa=0.051). This is the first report ofNeospora spp. antibodies in Brazilian donkeys and the first detection of antibodies against S. neurona in this animal species.

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Section: Resultsmentioning

confidence: 96%

“…and S. neurona exposure in donkeys (Equus asinus) from other countries is scarce (MACHACOVÁ et al, 2013;BLANCO et al, 2014). Donkeys are used traditionally for working roles, however in some parts of the world they are increasingly being used for milk production.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of antibodies against Neospora spp. and Sarcocystis neurona in donkeys from northeastern Brazil

Gennari

Pena

Lindsay

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…In a study performed in Italy, 11.8% of 238 donkeys were positive for Neospora spp. using a commercial competitive-inhibition ELISA (cELISA, VMRD, Inc., Pullman, WA, USA) for N. caninum (MACHAČOVÁ et al, 2013). In another study conducted in Iran, 100 sera from donkeys were tested by a Neospora modified agglutination test (N-MAT) and revealed 52% of reactive samples.…”

Section: Discussionmentioning

confidence: 99%

“…based on the detection of anti-Neospora sp. antibodies (GHAREKHANI et al, 2013;MACHAČOVÁ et al, 2013). In each of these reports, a single serologic method was used to test the animal sera.…”

Section: Introductionmentioning

confidence: 99%

Brazilian donkeys (Equus asinus) have a low exposure to Neospora spp.

Galvão

Rezende-Gondim

Chaves

et al. 2015

Rev. Bras. Parasitol. Vet.

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Donkeys (Equus asinus) are closely related to horses and are known to be infected by several equine pathogens. Neospora caninum and Neospora hughesi are protozoan parasites that infect horses, but they were not confirmed in donkeys up to this date. The aim of this study was to evaluate the exposure of donkeys (Equus asinus) to Neospora spp. using tachyzoites of N. caninum as antigen and employing two common serologic methods, IFAT and immunoblot. Sera from 500 donkeys were obtained from 30 municipalities in Bahia state and tested by IFAT. Two of 500 sera were positive for Neospora spp. by IFAT with antibody titers of 100, and recognized a 37kDa antigen in immunoblot. Approximately 22% of the samples showed strong apical reactions and/or incomplete fluorescence, what may cause confusion in the interpretation of IFAT. We concluded that Neospora spp. are possibly of minor importance for Brazilian donkeys. Future studies are necessary to prove that Neospora spp. can naturally infect donkeys.Keywords: Neospora, Equus asinus, donkeys, antibodies. ResumoAsininos (Equus asinus) são próximos filogeneticamente a equinos e podem ser infectados por vários patógenos de cavalos. Neospora caninum e Neospora hughesi são parasitos protozoários que infectam equinos, porém não foram confirmados em asininos até o momento. O objetivo deste estudo foi avaliar a exposição de asininos (Equus asinus) a Neospora spp., usando-se taquizoítos de N. caninum como antígeno e empregando-se duas técnicas sorológicas comuns para esta finalidade, reação de imunofluorescência indireta (RIFI) e immunoblot. Soros de 500 asininos, obtidos em 30 municípios no Estado da Bahia, foram testados por meio da RIFI. Dois dos 500 soros foram positivos para Neospora spp. pela RIFI com títulos de anticorpos de 100, e reconheceram um antígeno de 37kDa no immunoblot. Aproximadamente, 22% das amostras apresentaram fortes reações apicais e/ou fluorescência incompleta, o que pode causar confusão na interpretação da RIFI. Conclui-se que Neospora spp. são, possivelmente, de pouca importância para asininos brasileiros. Estudos posteriores são necessários para provar que Neospora spp. podem causar infecção natural em asininos.

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“…In another study in Brazil, researchers found antibodies to Neospora in 2 of 500 donkeys studied using IFAT (cut-off 1:100) [6]. Antibodies to Neospora were also reported in 11 (19.7%) of 56 donkeys from Colombia by using Dot-ELISA [2], in 52 (52%) of 100 donkeys from Iran by the Neospora agglutination test (NAT, cut-off 1:80) [8], and 28 (11.8%) of 238 donkeys from Italy by using competitive inhibition ELISA [12]. Again, comparison of seroprevalences among the studies should be interpreted cautiously because of different laboratory methods used to detect anti- Neospora antibodies.…”

Section: Discussionmentioning

confidence: 99%

Seroepidemiology ofSarcocystis neuronaandNeospora hughesiinfections in domestic donkeys (Equus asinus) in Durango, Mexico

et al. 2017

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There is currently no information regarding Sarcocystis neurona and Neospora hughesi infections in donkeys in Mexico. Here, we determined the presence of antibodies against S. neurona and N. hughesi in donkeys in the northern Mexican state of Durango. Serum samples of 239 domestic donkeys (Equus asinus) were assayed for S. neurona and N. hughesi antibodies using home-made enzyme-linked immunoassays; six (2.5%) of the 239 donkeys tested seropositive for S. neurona. The seroprevalence of S. neurona infection was comparable among donkeys regardless of their origin, health status, or sex. Multivariate analysis showed that seropositivity to S. neurona was associated with increased age (OR = 2.95; 95% CI: 1.11–7.82; p = 0.02). Antibodies to N. hughesi were found in two (0.8%) of the 239 donkeys. Both exposed donkeys were healthy, 3- and 6-year-old females. This is the first evidence of S. neurona and N. hughesi infections in donkeys in Mexico.

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Seroprevalence and risk factors of Neospora spp. in donkeys from Southern Italy

Cited by 24 publications

References 13 publications

Prevalence of antibodies against Neospora spp. and Sarcocystis neurona in donkeys from northeastern Brazil

Prevalence of antibodies against Neospora spp. and Sarcocystis neurona in donkeys from northeastern Brazil

Brazilian donkeys (Equus asinus) have a low exposure to Neospora spp.

Seroepidemiology ofSarcocystis neuronaandNeospora hughesiinfections in domestic donkeys (Equus asinus) in Durango, Mexico

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