Seroprevalence and Molecular Epidemiology of Brucellosis in Cattle in Egypt

Hady, Ahmed M El; Ahmed, Mohamed Sayed

doi:10.4172/2329-888x.1000153

Cited by 7 publications

(10 citation statements)

References 22 publications

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“…A higher number of seropositive pigs was recorded by c-ELISA (10.8%) when compared to the i-ELISA (4.83%). Although these samples were not taken following the sampling plan of the Egyptian prevalence study plan of ruminants, the ranges are in agreement with the previous prevalence reports of brucellosis in cattle, buffaloes, sheep, and goats in Egypt [7,10,34].…”

Section: Discussionsupporting

confidence: 86%

Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt

et al. 2019

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Brucellosis is considered as endemic disease of animals and humans since thousands of years in Egypt. However, brucellosis in pigs has never been reported in Egypt. Thus, serological and molecular assays were applied to detect anti-Brucella antibodies and DNA in serum samples collected from pigs. In total 331 blood samples collected from male and female pigs at slaughterhouses of Cairo and Giza governorates were investigated using Brucella c- and i-ELISA and Brucella real-time PCR. Anti-Brucella antibodies were detected in 16 (4.83%) and 36 (10.8%) sera by i-ELISA and c-ELISA, respectively. Brucella DNA was detected in 10 (3.02%) seropositive samples and identified as Brucella melitensis (7/10) and Brucella suis (3/10). A higher prevelance was found in boars. This is the first study investigating pig brucellosis in Egypt. The results of this study will raise awareness for brucellosis in these farm animals and will help to develop effective control strategies.

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Section: Discussionsupporting

confidence: 86%

Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt

et al. 2019

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“…This may explain the occurrence of this biotype in camels in the current study which consider the most dominant biotype of Brucella isolated from both animals and human in Egypt as reported by (Mohamed and Eisa, 2004;Soliman, 2006;El-Diasty, 2009;El-Sayed et al, 2011;Abdel Hamid, 2012;Afifi et al, 2015, El-Diasty et al, 2016, El-Hady et al, 2016.…”

Section: Discussionsupporting

confidence: 48%

“…However, information about economic losses due to camel brucellosis is scarce. Although camels are not the primary host of Brucella, B. abortus and B. melitensis isolated from milk, aborted fetus, lymph nodes and vaginal swabs (Radwan et al, 1992;Gameel et al, 1993;Agab et al, 1994;Abou-Eisha, 2000;Hamdy and Amin, 2002;El-Gohary et al, 2016;El-Diasty et al, 2016;El-Hady et al, 2016). Disease transmission depends on Brucella spp.…”

Section: Introductionmentioning

confidence: 99%

Sero-prevalence of camel brucellosis (Camelus dromedarius) and phenotypic characteristics of Brucella melitensis biovar 3 in Shalateen City, Red Sea Governorate, Egypt

El‐Sayed¹,

Sayed-Ahmed²,

El-Diasty³

et al. 2017

Afr. J. Microbiol. Res.

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The objective of this study was to estimate the sero-prevalence of brucellosis in camels in Shalateen city, Red sea Governorate. A total of 801 Sera were collected from apparently healthy dromedary camels from 2014 to 2015 spring. Sera were consequently serologically tested and confirmed using Rose Bengal plate test (RBPT), buffer acidified plate antigen test (BAPAT) and complement fixation test (CFT). 103 (12.90%), 93 (11.60%), and 92 (11.50%) were positive for RBPT, BABAT and CFT, respectively. Young camels were more sero-positive than old one (13.30 vs.10.80%). In addition, females were more sero-positive than males (19.10 vs. 7.10%). Moreover, Brucella melitensis biovar 3 was isolated from stomach content of aborted camel fetus. Statistically, the apparent prevalence (AP) was estimated to be 11.50%, while true prevalence (TP) was 13.60% (95% CI: 11.20 to 16%; P < 0.05). There was non statistical significant association between different age groups, while a highly significant difference were detected between seasons and genders. This study documented a high prevalence of camel brucellosis in the area of study and there is a need for planning and implementation of joint programs by stakeholders in prevention and control of the disease as well as raising public awareness in decreasing the distribution of the disease.

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“…Such inter-species transmission situation may be the outcome of close contact between ewes, goats and cows [34]. This may illustrate the occurrence of this biotype in cows and ewes in the current study which consider the most dominant Brucella biotype in Egypt [35][36][37][38].…”

Section: Resultsmentioning

confidence: 77%

Hematological, Biochemical and Immunological Studies on Brucellosis in Cows and Ewes in Dakahlia and Damietta Governorates, Egypt

Hashem

El-Mandrawy

El-Diasty³

et al. 2020

Zagazig Veterinary Journal

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Brucellosis is an important disease of public health significance that leads to severe losses. The current study was carried out to identify the recovered Brucella species , and its prevalence in cows and ewes in Dakahlia and Damietta Governorates during May 2018 to March 2019, with the hemato-biochemical analyses in the positive cases. In the current study, B. melitensis biovar 3 was identified with a total prevalence of 8.75% and 6.98% in cows and ewes, respectively. Hematological results denoted a normocytic normochromic anemia in infected cows and ewes at the 2 nd and 4 th weeks after abortion group (GP) 2 and GP.3, respectively, with leucocytosis especially in GP.3. Significant hypoproteinemia and hypoalbuminemia were detected in infected cows at the 2 nd week after abortion, with non-significant changes at 4 th week after abortion. While Brucellosis-infected ewes showed a significant (P < 0.05) hypoproteinemia and hypoalbuminemia at both 2 nd and 4 th weeks post abortion. The serum AST, ALT, ALP and LDH activities and levels of IL-6 and TNF-α were markedly elevated (P < 0.05) in both infected cow and ewes with markedly lowered serum urea level. Whereas serum glucose level was significantly decreased (P < 0.05) in infected cows (GPs 2 and 3), as well as, infected ewes of GP. 2, with non-significant changes in infected ewes of GP3. The serum creatinine level showed non-significant changes in all infected cows and ewes. Serum immunoglobulin M was significantly increased in both infected cows and ewes of GPs 2 and 3, while immunoglobulin G was significantly elevated in both infected cow and ewes of GP3. Moreover, phagocytic percent and index showed a significant decrease in both infected cows and ewes (GPs2 and 3). Thus, evaluations of hematological, biochemical and immunological parameters together with specific diagnostic tests are necessary to obtain more reliable results for the diagnosis of Brucellosis in cows and ewes.

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Seroprevalence and Molecular Epidemiology of Brucellosis in Cattle in Egypt

Cited by 7 publications

References 22 publications

Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt

Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt

Sero-prevalence of camel brucellosis (Camelus dromedarius) and phenotypic characteristics of Brucella melitensis biovar 3 in Shalateen City, Red Sea Governorate, Egypt

Hematological, Biochemical and Immunological Studies on Brucellosis in Cows and Ewes in Dakahlia and Damietta Governorates, Egypt

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