Seroprevalence and epidemiology of Toxoplasma gondii in farm animals in different regions of Egypt

Fereig, Ragab M.; Mahmoud, Hassan Y.A.H.; Mohamed, Samy G.A.; AbouLaila, Mahmoud; Abdel-Wahab, A.; Osman, Salama A.; Zidan, Sherif; El-Khodary, S. A.; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi

doi:10.1016/j.vprsr.2016.05.002

Cited by 33 publications

(48 citation statements)

References 44 publications

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“…A possible explanation for the high OD in this study could be the unspeci c epitopes of this antigen in the amino-terminal region [31] The infection rate in our study was lower than that of 27.9% in a previous report on goats in Satun province, Thailand [29]. The difference of the seroprevalence rate may be attributable to the use of different serological diagnosis techniques (iELISA and LAT) and different sampling areas.…”

Section: Discussioncontrasting

confidence: 64%

“…In cats, a single GRA7 recombinant protein [26] and a mixture of recombinant GRA2, GRA6, GRA7, and GRA15 [27] were used to determine the prevalence of T. gondii infection in China and Japan, respectively. Moreover, recombinant GRA7 protein-based ELISA has been used in seroprevalence studies of farm animals in Egypt [28] and Thailand [29]. To date, only one study describing the use of TgGRA8 together with recombinant GRA7 to detect speci c IgG antibodies against the parasite was published in Turkey [30].…”

Section: Discussionmentioning

confidence: 99%

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Development and Evaluation of Recombinant GRA8 Protein for the Serodiagnosis of Toxoplasma Gondii Infection in Goats

Jirapattharasate

Udonsom

Prachasuphap

et al. 2020

Preprint

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Background The development of sensitive and specific methods for detecting Toxoplasma gondii infection is critical for preventing and controlling toxoplasmosis in humans and other animals. Recently, various recombinant proteins have been used in serological tests for diagnosing toxoplasmosis. The production of these antigens is associated with live tachyzoites obtained from cell cultures or laboratory animals for genomic extraction to amplify target genes. Synthetic genes have gained a key role in recombinant protein production. For the first time, we demonstrated the production of the recombinant protein of the T. gondii dense granular antigen 8 (TgGRA8) gene based on commercial gene synthesis. Recombinant TgGRA8 plasmids were successfully expressed in an Escherichia coli system. The recombinant protein was affinity-purified and characterized via sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. Furthermore, the diagnostic potential of the recombinant protein was assessed using 306 field serum samples from goats via indirect enzyme-linked immunosorbent assay (iELISA) and the latex agglutination test (LAT).Results Western blotting using known positive serum samples from goats identified a single antigen at the expected molecular weight of TgGRA8 (27 kDa). iELISA illustrated that 15.40% of goat samples were positive for T. gondii-specific IgG antibodies. In addition, TgGRA8 provided high sensitivity and specificity, with significant concordance (91.83) and kappa values (0.69) compared with the results obtained using LAT.Conclusion Our findings highlight the production of a recombinant protein from a synthetic TgGRA8 gene and the ability to detect T. gondii infection in field samples. The sensitivity and specificity of TgGRA8 demonstrated that this protein could be a good serological marker for detecting specific IgG in goat sera.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of Recombinant GRA8 Protein for the Serodiagnosis of Toxoplasma Gondii Infection in Goats

Jirapattharasate

Udonsom

Prachasuphap

et al. 2020

Preprint

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“…In Egypt, numerous investigations exhibited late abortion due to T. gondii in small ruminant with sero-prevalence of 47.8% and 51.4% in sheep and 35.1% and 39.4% in goat utilizing Latex Agglutination Test (LAT) and ELISA individually (Fereig et al 2016), Moreover, Hasanain et al (2013) demonstrated that 61.4%, 80.4%, 34.4% and 19.3% of sheep, swine, buffaloes and cows in Egypt were positive for toxoplasmosis. The high level of toxoplasmosis in the Egyptian farm animals discloses that up to 57.1% of ready to eat Egyptian meat meals were positive to toxoplasmosis using PCR as endorsed by Abd El-Razik et al (2014).…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence, isolation, molecular detection and genetic diversity of Toxoplasma gondii from small ruminants in Egypt

et al. 2018

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Toxoplasmosis is an infectious zoonotic disease caused by protozoan Toxoplasma gondii. Detection of T. gondii infection with touchy and particular strategies is a key advance to control and prevent toxoplasmosis. Genotyping can explain the virulence, epidemiology and setting up new methodologies for diagnosis and control in human and animals. The point of this study was to assess the seroprevalence of T. gondii in sheep and goat in Egypt and to comprehend the genetic variety of T. gondii isolates circling in Egypt. Blood samples were gathered from 113 ewes and 95 she-goats from three Egyptian governorates (Cairo, Giza and Al-Sharkia). Also blood and tissue samples were gathered from 193 sheep and 51 goats from Cairo and Giza abattoirs. All samples were assayed serologically utilizing ELISA and OnSite Toxo IgG/IgM Rapid test cassettes (OTRT) tests and the tissue samples of the seropositive animals were digested and microscopically examined then bio-assayed in mice as viability test. All the T. gondii isolates undergo molecular identification using PCR and genotyped utilizing nPCR/RFLP analysis of SAG2 gene. The total seropositivity of live sheep and goat was 47.15 and 39.2% utilizing ELISA and OTRT respectively. Concerning abattoirs, seropositivity, positive microscopic examination, mice viability from sheep samples were 47.1%, 37.3% and 44.1% respectively while that of goats were 45.5%, 33.3% and 48.6% respectively. Eighteen T. gondii isolates were affirmed utilizing PCR. Genotyping confirmed 10 isolates (55.5%) as type II, 6 (33.3%) as type III and 2 (11.1%) as atypical genotypes. Type II and III are the genotypes mostly circling among small ruminants in Egypt and this is most significance for the public health in Egypt.

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“…In cats, a single GRA7 recombinant protein [27] and a mixture of recombinant GRA2, GRA6, GRA7, and GRA15 [28] were used to determine the prevalence of T. gondii infection in China and Japan, respectively. Moreover, recombinant GRA7 protein-based ELISA has been used in seroprevalence studies of farm animals in Egypt [29] and Thailand [30]. To date, only one study describing the use of TgGRA8 together with recombinant GRA7 to detect speci c IgG antibodies against the parasite was published in domestic turkeys [31].…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of recombinant GRA8 protein for the serodiagnosis of Toxoplasma gondii infection in goats

Jirapattharasate

Udonsom

Prachasuphap

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundThe development of sensitive and specific methods for detecting Toxoplasma gondii infection is critical for preventing and controlling toxoplasmosis in humans and other animals. Recently, various recombinant proteins have been used in serological tests for diagnosing toxoplasmosis. The production of these antigens is associated with live tachyzoites obtained from cell cultures or laboratory animals for genomic extraction to amplify target genes. Synthetic genes have gained a key role in recombinant protein production. For the first time, we demonstrated the production of the recombinant protein of the T. gondii dense granular antigen 8 (TgGRA8) gene based on commercial gene synthesis. Recombinant TgGRA8 plasmids were successfully expressed in an Escherichia coli system. The recombinant protein was affinity-purified and characterized via sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. Furthermore, the diagnostic potential of the recombinant protein was assessed using 306 field serum samples from goats via indirect enzyme-linked immunosorbent assay (iELISA) and the latex agglutination test (LAT).ResultsWestern blotting using known positive serum samples from goats identified a single antigen at the expected molecular weight of TgGRA8 (27 kDa). iELISA illustrated that 15.40% of goat samples were positive for T. gondii-specific IgG antibodies. In addition, TgGRA8 provided high sensitivity and specificity, with significant concordance (91.83) and kappa values (0.69) compared with the results obtained using LAT.ConclusionOur findings highlight the production of a recombinant protein from a synthetic TgGRA8 gene and the ability to detect T. gondii infection in field samples. The sensitivity and specificity of TgGRA8 demonstrated that this protein could be a good serological marker for detecting specific IgG in goat sera

show abstract

Seroprevalence and epidemiology of Toxoplasma gondii in farm animals in different regions of Egypt

Cited by 33 publications

References 44 publications

Development and Evaluation of Recombinant GRA8 Protein for the Serodiagnosis of Toxoplasma Gondii Infection in Goats

Development and Evaluation of Recombinant GRA8 Protein for the Serodiagnosis of Toxoplasma Gondii Infection in Goats

Seroprevalence, isolation, molecular detection and genetic diversity of Toxoplasma gondii from small ruminants in Egypt

Development and evaluation of recombinant GRA8 protein for the serodiagnosis of Toxoplasma gondii infection in goats

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