Seronegative cat-scratch disease diagnosed by PCR detection of Bartonella henselae DNA in lymph node samples

Chondrogiannis, Konstantinos; Vezakis, Αntonios; Derpapas, Michael; Melemeni, Aikaterini; Fragulidis, Georgios P.

doi:10.1590/s1413-86702012000100019

Cited by 7 publications

(7 citation statements)

References 21 publications

(34 reference statements)

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“…Although serology, culture, and PCR remain the mainstays for diagnosing bartonellosis in dogs, clinicians and diagnosticians should recognize that each of these assays lacks sensitivity, specificity, or both . These limitations adversely influence seroepidemiological conclusions; potentially result in the transfusion of Bartonella spp.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cell culture‐grownBartonellaantigens in immunofluorescent antibody assays for the serological diagnosis of bartonellosis in dogs

Neupane

Hegarty

Marr

et al. 2018

Veterinary Internal Medicne

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BackgroundBecause of poor sensitivity and questionable specificity of immunofluorescent antibody assays (IFAs), serological diagnosis of Bartonella species infections in dogs remains challenging. Despite limitations, IFA testing is the historical “gold standard” for Bartonella serodiagnosis in animals and humans. Because most diagnostic laboratories test against only 1 or 2 Bartonella spp., testing against a broader panel of Bartonella antigens may enhance diagnostic sensitivity and specificity.ObjectiveTo evaluate the sensitivity and specificity of Bartonella IFA using 8 cell culture‐grown Bartonella spp. isolates.AnimalsArchived serum samples from 34 Bartonella spp. naturally exposed, polymerase chain reaction (PCR)‐positive dogs and from 26 PCR‐negative and IFA‐negative dogs.Methods Bartonella IFA sensitivity and specificity were assessed using cell culture‐grown whole cell antigens derived from 3 Bartonella henselae (Bh) strains (Bh Houston 1, Bh San Antonio Type 2, Bh California 1), 3 Bartonella vinsonii subsp. berkhoffii genotypes (Bvb I, II, and III), Bartonella koehlerae (Bk), and Bartonella quintana (Bq).ResultsOnly 62% of 34 Bartonella spp. PCR‐positive dogs were seroreactive to any of the 8 Bartonella IFA antigens, indicating low IFA sensitivity. PCR‐positive dogs were most often IFA seroreactive to Bq (n = 15), to Bvb II (n = 13), or to both (n = 9) antigens. Of the 26 previously IFA‐negative/PCR‐negative dogs, 4 (15%) were seroreactive using the expanded antigen panel.Conclusion and Clinical ImportanceDespite IFA testing of dogs against 8 different Bartonella isolates, IFA sensitivity remained poor, and specificity was only 85%. Development of a reliable serological assay is needed to facilitate the diagnosis of Bartonella infection in dogs.

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Section: Discussionmentioning

confidence: 99%

Evaluation of cell culture‐grownBartonellaantigens in immunofluorescent antibody assays for the serological diagnosis of bartonellosis in dogs

Neupane

Hegarty

Marr

et al. 2018

Veterinary Internal Medicne

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“…La detección de ADN por RPC obtenidos por biopsia ganglionar sería la prueba confirmatoria ideal en los casos de alta presunción y serologías negativas 20 .…”

Section: Discussionunclassified

Enfermedad por arañazo de gato como causa de fiebre de origen desconocido

Santarcángelo

Sosa

Dondoglio

et al. 2013

Rev. chil. infectol.

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The cat scratch disease is a relatively rare disease caused by Bartonella henselae. Only a small proportion of the cases that described extranodal involvement occurs. We report a 11 year old male patient with persistent fever, liver and osteoarticular involvement with a predominance of dorsolumbar spine. Evolution was satisfactory with complete clinical and imaging evidenced recovery in several months of monitoring. Case was analyzed considering EAG published literature, emphasizing the need to include this condition in the differential diagnoses in patients with bone involvement suggestive of systemic disease.

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“…La [11,[13][14][15]. Selon une étude rétrospective menée à Angers, l'association d'une sérologie et d'une PCR permettrait d'obtenir une sensibilité de 92 % [13].…”

Section: Discussionunclassified