Serology versus ARMS-PCR in prospective HLA-class I typing for bone marrow transplantation

Abstract

While HLA class II alleles identification by means of complement mediated lymphocytotoxicity (serology) is almost replaced by DNA typing techniques, serology is still widely used for routine class I typing. The aim of this prospective study was to compare PCR-based Amplification Refractory Mutation System with serology in clinical HLA class I alleles assignment in patients receiving marrow transplants and their potential donors. The total discrepancy rate in 114 consecutively typed individuals for HLA-A and HLA-C alleles was only in favor of ARMS-PCR, whereas HLA-B typing was discrepant also in favor of serology. The discrepancies were higher in patients, particularly in those with acute lymphoblastic leukaemia, than in healthy individuals. We conclude, that ARMS-PCR is clearly superior to serology in definition of class I alleles, which might be of clinical importance particularly for bone marrow transplantation.

HLA–B51/B5 and the risk of Behçet's disease: A systematic review and meta‐analysis of case–control genetic association studies

“…However, these calculations might have been under-powered in our analyses. The observed trend toward a significant variation according to genotyping technique is counterintuitive in light of the relatively small misassignment rates reported for HLA class 1 typing with serologic compared with molecular techniques (109,110). …”

Diagnostic Accuracy of PCR-Based Detection Tests for Helicobacter Pylori in Otitis Media: A Meta-Analysis

“…However, the serology and histologic identification are not fast and reliable enough [29][30][31][32][33].The PCR-based molecular diagnostic techniques have been used for testing H. Pylori infections for about a decade [34,35]. Our meta-analysis revealed some concerns using the available evidences, which basically reflects the experience with PCR-based molecular diagnosis of H. Pylori infection among patient with OME, including the limitation of available evidences and a considerable variability among the available studies.…”

Análise molecular de polimorfismos e expressão gênica do fator β1 de transformação do crescimento (TGF-β1) no câncer de próstata.

“…Gleason (1996), que valoriza, principalmente, o padrão glandular e a relação entre as glândulas e o estroma prostático. Nesse sistema, os tumores são classificados em 5 graus, denominando-se grau 1 as lesões mais diferenciadas e grau 5 as mais indiferenciadas.…”

“…A incidência do câncer de próstata aumenta com a idade, mais do que em diversos outros tipos de cânceres (COFFEY, 1993;ISAACS, 1997;CHAN et al, 1998;INCA 2004 (TSUKAMOTO et al, 1998;ZACCHEO et al, 1998; MAKRIDAKIS et a., CASELLA et al, 2001 1996).…”