SEROLOGY OF NEISSERIA GONORRHOEAE. DEMONSTRATION BY CO‐AGGLUTINATION AND IMMUNOELECTROPHORESIS OF ANTIGENIC DIFFERENCES ASSOCIATED WITH COLOUR/OPACITY COLONIAL VARIANTS

Abstract

of Neisseriu gonorrhoeue. Demonstration by co-agglutination and immunoelectrophoresis of antigenic differences associated with colour/opacity colonial variants.Acta path. microbiol. scand. Sect. B, 88: 39-46, 1980. Serological classification of Neisseriu gonorrhoeue by co-agglutination (COA) into previously described antigen classes W and J was confirmed in the present work. Immunization of rabbits with classified organisms gave antibodies which produced the expected results when used in the preparation of COA reagents. Colour /opacity colonial variants of isogenic strains, characterized by stereomicroscopy as opaque and transparent, respectively, were found to influence immunization, absorption, coagglutination and precipitation in immunoelectrophoresis (IE). It was shown by COA and IE that organisms of opaque colonies often contained an extra antigenic factods) which resisted heating at 100 OC but was sensitive to treatment with pronase. They were extracted by heating the organisms in saline or lithium chloride solution. Serological classification with COA of clinical isolates was reproducible with reagents for antigen classes W and J. Colony morphology dependent reactions, mostly due to organisms of opaque colonies, occurred in 7 % with reagents for antigen class W and in 20% for antigen class J.

SEROLOGY OF NEISSERIA GONORRHOEAE. CLASSIFICATION BY CO‐AGGLUTINATION

“…Some of the class J reactions might well fit into class W, for example the reactions of D-4 and V-1 5 , T-1 3 and U-14 and F-6, which are pronase-stable. Many of the class J reactions have also been found to be dependent on colony morphology (9). The possible relationship of class J to the MOMP serotype system of Johnston et ul.…”