Serology of Acquired Hemolytic Anemias

Weiner, William J.; Vos, G. H.

doi:10.1182/blood.v22.5.606.606

Cited by 113 publications

(34 citation statements)

References 7 publications

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“…A similar case where an auto-anti-e was found in the serum of a healthy pregnant woman has been described by METAXAS, METAXAS-BUHLER and ROMANSKI [6] .The anti-e did have a slight blocking effect on her ccddee cells, but was only reactive with enzyme-treated cells. WEINER and VOS [12] report that anti-LW has never been detected in the autoantibodies of patients suffering from acquired haemolytic anaemia, even though it might well have been expected owing to the close relationship of LW and Rh antigens.…”

Section: Discussionmentioning

confidence: 98%

A Complex Serological Investigation Involving LW

Giles

Lundsgaard²

1967

Vox Sanguinis

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Section: Discussionmentioning

confidence: 98%

A Complex Serological Investigation Involving LW

Giles

Lundsgaard²

1967

Vox Sanguinis

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“…An eluate made from those cells reacted as shown in Table I. The cells were subjected to the partial heat elution method that avoids total red cell destruction (Stratton & Renton, 1958;Morel et al, 1978). They were then used in an attempt to adsorb the anti-Hr/hrB-like antibodies present in a sample of serum saved from the patient's first episode of haemolytic anaemia.…”

Section: Resultsmentioning

confidence: 99%

Atypical presentation of acute phase, antibody‐induced haemolytic anaemia in an infant

et al. 1982

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We describe a case of 'warm' antibody-induced haemolytic anaemia (WAIHA) in which marked depression of red cell Rh antigen expression resulted in the patient presenting with severe anaemia but a negative direct antiglobulin test (DAT). The serum contained potent IgG Rh antibodies. Unlike two previously reported cases (Koscielak, 1980; Veer et al. 1981) in which the diagnosis of WAIHA was established before the DAT became negative, this patient presented with negative serological findings during his first episode of anaemia. As a result, the serum antibodies appeared to be allo- not autoimmune in nature and to be unrelated to the patient's anaemia. Confirmation of the autoimmune nature of the Rh antibodies was not possible until nearly 2 years after the first episode of anaemia.

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“…In humans, a similarly varied picture has been revealed by investigations lo determine the blood group specificity of autoantibodies from patients with AIHA (reviewed by Mollison, Engelfriet & Contreras, 1987). These studies describe apparent diflferences between cases in the identity of the autoantigens, although often antibodies appear to have specificity within the Rhesus blood group system (Weiner & Vos, 1963).…”

Section: Discussionmentioning

confidence: 99%

“…The RhD antigen is associated with membrane components of 28-33 kD (Gahmberg. 1982;Moore, Woodrow & McClelland, 1982) and 45-100 kD (Moore & Green, 1987), and human AIHA autoantibodies often appear to have specificity within the Rhesus system (Weiner & Vos, 1963). Although no Rhesus equivalent has been described in thedog, the complex appears to play an important role in maintaining erythrocyte viability (Ridgewell et al, 1983) and so might be expected to be conserved between species.…”

Section: Discussionmentioning

confidence: 99%

Identification of autoantigens in canine autoimmune haemolytic anaemia

Barker

Gruffydd-Jones

Stokes

et al. 1991

Clinical and Experimental Immunology

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SUMMARYAutoantigens in canine autoimmune haemolytie anaemia (AlH A) were identified by immunoprecipitation using autoantibody eluted from the erythrocytes of affected dogs. At least three patterns of precipitated antigen were identified in six cases of AIHA. The most commonly precipitated antigen pattern was a combination of 42-kD and 29-kD peptides. associated with up to three oiher membrane components. These autoantigens may be canine glycophorins. which are of similar molecular mass, or may possibly represeni an equivateni ofthe human Rhesus comptex. An autoanligen identical in molecular mass to band 3. the erythrocyte anion channct proiein. was precipitated in one ease of AIHA, and unknown peptides of37kD and lOOkD were isolated by auloantibody from another dog. In one ease, no antigens were precipitated by the eluted antibody, indicating that the autoantibody may have bound a non-protein membrane component such as phospholipid. Overall it is considered that the different patterns observed may reflect differences in the aetiology ofthe condition. In other studies, sera from dogs with AIHA failed to immunoprecipitate autoantigens. bul were shown by immunoblotting to eontain autoantibodies to proteins of the erythroeyte cytoskeleton. Such autoantibodies were also demonstrated in normal canine sera and it is suggested that they arc unlikely to play a role in the pathogenesis of AIHA, but may be part of a normal clearance mechanism for damaged red blood cells.

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Serology of Acquired Hemolytic Anemias

Cited by 113 publications

References 7 publications

A Complex Serological Investigation Involving LW

A Complex Serological Investigation Involving LW

Atypical presentation of acute phase, antibody‐induced haemolytic anaemia in an infant

Identification of autoantigens in canine autoimmune haemolytic anaemia

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