Serology and PCR for bovine leptospirosis: herd and individual approaches

Otaka, Denis Y.; Martins, Gabriel; Hamond, Camila; Penna, Bruno; Medeiros, Marco Alberto; Lilenbaum, Walter

doi:10.1136/vr.100490

Cited by 52 publications

(29 citation statements)

References 6 publications

(22 reference statements)

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“…2) demonstrated that out of 89 seropositive cows, 23 responded positively in PCR, whereas Leptospira were found only in 11 urine samples. Similar studies were conducted by many researchers (20), who observed negative reaction in bacteriological studies, but positive in 50% cases using PCR (13). In the studies of these authors, PCR demonstrated very good results as compared to traditional methods, which was also confirmed in our study.…”

Section: Discussionsupporting

confidence: 80%

Leptospirosis in Cattle From Markets of Almaty Province, Kazakhstan

Kirkimbayeva

Łozowicka

Biyashev

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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This paper is the first study of the prevalence of leptospirosis in the cattle at slaughter from a rural area of Kazakhstan. Five hundred and seventy three samples of serum, urine, and kidneys from cattle of Alatau, Kazakh white and Auliyekol breed, aged from 2 to 5 years (unknown vaccination status), from the province of Almaty in the South-Eastern region were collected during four years (March 2010 to October 2013). The serological, bacteriological, and molecular analyses were performed. Serum samples were tested with 14 reference Leptospira serovars by microscopic agglutination test (MAT). MAT results showed that 89 (15.53%) serum samples had detectable antibodies against seven serovars of L. interrogans at a dilution of ≥1:100. Serovars: Pomona (38.2%), Tarassovi (27.2%), and Kabula (18.8%) were the most prevalent and their titres ranged from 100 to 1200. The spirochetes were detected in 11 samples of urine and nine samples of kidneys under dark-field microscope observation. The pure cultures were obtained from three samples. PCR technique confirmed leptospirosis in 23 out of 89 urine samples from cows, which showed the presence of leptospiral antibodies in microagglutination test. The high disease prevalence in cows indicates the high Leptospira contamination in this area. It was concluded that the bovine leptospirosis is an endemic and locally widespread disease in Kazakhstan, and that it may play a role in zoonotic transmission to humans.

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Section: Discussionsupporting

confidence: 80%

Leptospirosis in Cattle From Markets of Almaty Province, Kazakhstan

Kirkimbayeva

Łozowicka

Biyashev

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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“…Among other species and approaches, since the 1990’s it has been studied rats ( Rattus norvegicus ) captured from an urban area [17] and dogs with clinical indications of the disease [8,18]. In relation to livestock, it was studied cows [9,19,20], horses [21-23], goats [4,24], sheep [4] and pigs [25]. Wild mammals kept in the local zoo were also studied [26], as well as golden-lion tamarins from a research center [27] and wild felines [28].…”

Section: Introductionmentioning

confidence: 99%

The panorama of animal leptospirosis in Rio de Janeiro, Brazil, regarding the seroepidemiology of the infection in tropical regions

Martins

Lilenbaum

2013

BMC Vet Res

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BackgroundLeptospirosis is an important disease caused by various serovars of Leptospira sp. It can affect humans as well as domestic and wild animals; therefore, it has importance for public health, animal production, and wild species. The aim of this paper is to discuss the epidemiology of animal leptospirosis in Rio de Janeiro, Brazil, as a possible model for other tropical regions. In several studies conducted in the last 20 years, a total of 47 rats, 120 dogs, 875 cows, 695 horses, 1,343 goats, 308 sheep and 351 pigs from all regions of the state, in addition to 107 wild mammals and 73 golden-lion tamarins were tested (MAT) for anti-Leptospira antibodies.ResultsSeroreactivity was frequent in all studied species, confirming that the infection is endemic in Rio de Janeiro. Serogroups Icterohaemorrhagiae and Sejroe were the most prevalent in urban and rural scenarios, respectively. This paper reviews the current knowledge on animal leptospirosis in Rio de Janeiro and describes important differences between urban versus rural cycles of the infection in various species.ConclusionIdentification of the prevailing serogroups and their reservoirs is essential for understanding agent-host-environment interactions under tropical conditions.

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“…It is reported that, although serology is useful for herd diagnosis, it is not adequate for detection of individual carriers (Gamage et al, 2012;Hamond et al, 2014;Otaka et al, 2012) since seronegative animals also can shed leptospires. Therefore, an identification of carrier animals by direct tests like PCR or culture is more useful (Bomfim et al, 2008;Gamage et al, 2012 et al, 2012).…”

Section: Resultsmentioning

confidence: 99%