Serology and longevity of immunity against <i>Echinococcus granulosus</i> in sheep and llama induced by an oil‐based <scp>EG</scp>95 vaccine

Poggio, Thelma Verónica; Jensen, Oscar; Mossello, M.; Iriarte, Jorge; Avila, Héctor Gabriel; Gertiser, María Laura; Serafino, Juan Javier; Romero, Sandra Cuevas; Echenique, M. A.; Domínguez, David Ernesto Marón; Barrios, J. R.; Heath, D.D.

doi:10.1111/pim.12325

Cited by 11 publications

(5 citation statements)

References 18 publications

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“…New data confirm that the specific IgG responses to EG95 vaccination after the third or fourth vaccination induce an increased level of antibody greater than two immunizations and that this response is maintained longitudinally over time for at least 5 years. It would be sufficient time to avoid infection of the sheep and/or development of fertile cysts during the period of its life span in the region (Larrieu et al, ; Poggio et al, ).…”

Section: General Principles For Controlmentioning

confidence: 99%

Control of cystic echinococcosis: Background and prospects

Larrieu

Gavidia

Lightowlers

2019

Zoonoses and Public Health

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Cystic echinococcosis (CE), caused by Echinococcus granulosus, is a chronic and debilitating zoonotic larval cestode infection in humans, which is principally transmitted between dogs and domestic livestock, particularly sheep. Human CE occurs in almost all pastoral communities and rangeland areas of the underdeveloped and developed world. Control programmes against CE have been implemented in several endemic countries to reduce or eliminate the disease. New Zealand and Tasmania are examples of some of the first programmes to be undertaken (in insular territories) and which were very successful in the elimination of CE. The advent and proven effectiveness of praziquantel, plus the experience of insular models, produced high expectations for rapid advances in a second generation of control programmes undertaken in continental areas (Argentina, Uruguay and Chile). Nevertheless, only moderate gains in CE control have been made and the impact on prevalence among humans has been slight. A major impediment to the adoption of procedures that were successful in New Zealand and Tasmania has been the requirement to administer praziquantel to dogs in rural areas eight times per year over numerous years. In addition, there have been clear technological improvements made in the diagnosis of canine echinococcosis for surveillance, the genetic characterization of parasite strains and in vaccination against CE infection in livestock. In order to establish new paradigms and appropriate combinations of control strategies, we have carried out a review and discussion of the available control tools and control models. Control strategies must be suitable and sustainable to benefit the Echinococcosis–endemic areas primarily, which at the same time are the poorest regions of the world.

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Section: General Principles For Controlmentioning

confidence: 99%

Control of cystic echinococcosis: Background and prospects

Larrieu

Gavidia

Lightowlers

2019

Zoonoses and Public Health

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“…Serum samples were obtained from an EG95 vaccination study . Briefly, Echinococcus granulosus eggs were collected from infected dog stool, in an endemic zone of the mountain ranges of Chubut, Argentina, after arecoline treatment.…”

Section: Methodsmentioning

confidence: 99%

Detection of Echinococcus granulosus sensu lato infection by using extracts derived from a protoscoleces G1 cell line

Maglioco

Gentile²,

Venturi

et al. 2019

Parasite Immunology

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Cystic echinococcosis (CE), also called hydatidosis, is a zoonotic parasitosis worldwide distributed 1 caused by the larval stage (metacestode) of Echinococcus granulosus sensu lato. This hermaphrodite worm develops in the intestine of domestic and wild canids, and then its gravid proglottid releases the eggs to the faeces, contaminating the environment. The eggs ingested by the intermediate host hatch in the duodenum, and the oncosphere then penetrates the intestinal wall, migrates, and becomes trapped in the host's organs, where it becomes metacestode. The parasite's cycle is accomplished when the infected organ from the intermediate host is ingested by the definitive host (canids). 2 Humans, as intermediate accidental hosts, who are more exposed to the infection in unsanitary conditions, develop a parasitic disease involving the encystment of the larvae in different organs (the liver in 70%-80% of the cases, the lungs in 20%-30% of the cases and other organs with lower frequency), 3 resulting in a wide spectrum of clinical manifestations. Mitochondrial DNA has so far allowed identifying eight Echinococcus granulosus sensu lato genotypes (G1, G3-G8, and G10). 4,5 Four of these genotypes, G1 in sheep, G5 in cattle, G6 in camelids and G7 in swine, have been detected in Argentina. 4 Abstract Cystic echinococcosis (CE) can be diagnosed by means of several serological approaches, but their results vary among laboratories due to the molecular characteristics of the reference antigens used. Thus, this study aimed to address both the relevance of an EGPE cell line previously obtained from Echinococcus granulosus protoscoleces G1 and the complexity of the immune response by using two different in vitro growth stages as separate sources of parasite antigens. The serum reactivity was investigated by western blotting (WB) in 21 CE patients from an endemic area in a matched case-control design and also in seven experimentally infected sheep and five healthy control sheep. EGPE-antigen-human serum sensitivity by WB was higher than that of hydatid fluid (HF) WB, ELISA and DD5 (P < .05, Chi-square test). EGPE protein extract was immunogenic in mice and hyperimmune plasma reacted with HF proteins, and AgB2 expression was detected by molecular analysis. Proteins of 37 to 60 kDa were recognized by 95.24% of the CE patients' sera but, with poor specificity. Statistically significant differences were found between serum protein extract recognition at 7 and 20 days of cell growth. The EGPE cell line is a laboratory source of antigens for improvement of CE serological diagnosis. K E Y W O R D S cell line extract, Cystic echinococcosis, human, immunoblot, parasite, sera, sheep

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“…La vacuna producida en Argentina con adyuvante oleoso logró mejor protección que la vacuna con adyuvante acuoso. La protección inducida por la vacuna oleosa producida en Argentina y la vacuna experimental producida en Australia, fueron equivalentes (Heath, 2012;Poggio, 2016).…”

Section: -Comparación De Distintas Formulaciones Y Adyuvantes En Ovinosunclassified

Análise Dos Riscos Da Automedicação E a Prevalência Desse Hábito Entre Os Acadêmicos Da Faculdade Unicesumar Campus Ponta Grossa

Prado¹

2021

O Fortalecimento Intensivo Das Ciências Biológicas E Suas Interfaces 2

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Direitos para esta edição cedidos à Atena Editora pelos autores. Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0).O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica.A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético.

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Serology and longevity of immunity against Echinococcus granulosus in sheep and llama induced by an oil‐based EG95 vaccine

Cited by 11 publications

References 18 publications

Control of cystic echinococcosis: Background and prospects

Control of cystic echinococcosis: Background and prospects

Detection of Echinococcus granulosus sensu lato infection by using extracts derived from a protoscoleces G1 cell line

Análise Dos Riscos Da Automedicação E a Prevalência Desse Hábito Entre Os Acadêmicos Da Faculdade Unicesumar Campus Ponta Grossa

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