Serologically Defined and Lymphocyte-Defined Components of the Major Histocompatibility Complex in the Mouse

129

The importance of cell interaction among lymphoid cells in the development of immune responses is well established. In humoral responses bone marrowderived (B) lymphocytes are recognized to be precursors of the cells that release serum antibody (1-4). Thymus-derived (T) lymphocytes, on the other hand, do not develop into antibody-forming cells (5) but play an important role in primary antibody responses by cooperating with B cells (6, 7).In contrast, cellular immunity is thought to be mediated by T lymphocytes, apparently without collaboration with B lymphocytes. However there is increasing evidence to suggest that cooperative interaction occurs among subsets of T lymphocytes (8-11). For example, Cantor and Asofsky proposed that a Ti-T~ cell interaction takes place during the initiation of graft-vs.-host responses in F1 neonates (8,9).Cytotoxic in vitro allograft responses have been shown to be mediated by T lymphocytes (12, 13). As such, they provide a convenient tool for analyzing the cellular requirements necessary for such a response to occur. Experiments therefore were designed to evaluate in vitro the concept of T-T cell interaction in cellular immunity against transplantation antigens. The results obtained are compatible with the idea that two cell types interact with each other in the initiation of such a response. Moreover, evidence is provided that one cell type acts mainly as helper cells allowing precursor cells of cytotoxic lymphocytes (CL) i to be immunized effectively.

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Synergy During in Vitro Cytotoxic Allograft Responses

Wagner

1973

129

“…This region controls in codes for: (a) H-2-linked immune response genes (3) ; (b) I-region-associated (Ia) antigens, which are predominantly expressed on B cells and macrophages (14)(15)(16) ; (c) lymphocyte-determined antigens, which stimulate strong mixed lymphocyte responses (12) ; (d) cell interaction between T cells and B cells (4,13) ; and (e) a factor involved in cell cooperation, putatively released by T cells (17) .…”

Section: Discussionmentioning

confidence: 99%

The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens.

Erb¹,

Feldmann²

1975

346

Helper cell induction to nonparticle antigens in vitro requires the cooperation of T cells and macrophages, but does not occur if the macrophages are allogenic. The reasons for this were investigated. Malfunction of allogenic macrophages was excluded by cultures with their syngenic T cells; suppressor cell induction was excluded by admixture experiments. Thus, T cells and macrophages only cooperated if they were genetically similar. The genetic locus (loci) involved was mapped. Using congenic lines differing only at the H-2 complex, the genetic control of T-macrophage interaction was localized in the H-2 region. Mice with intra H-2 recombinants were used to map the T-macrophage interaction locus in the I-A region of the H-2 complex (formerly known as poly-D, L-ala-poly-L-lys. Recombinants were also used to exclude the presence of another T-macrophage locus either the K, I-B, or I-C, SS-Slp, or D regions of the H-2 complex. Genetic restrictions for T-macrophage interaction in helper cell induction was shown in mice of the H-2-k, d, b, q, s genotypes as well as in H-2 recombinants. The possible mechanisms and significance of this genetic restriction are discussed.

“…In contrast to the wide tissue distribution of the H-2K and H-2D major histocompatibility antigens, the Ia antigens have a restricted distribution with principal expression on subpopulations of T and B lymphocytes (2)(3)(4)(5)(6)(7)(8). The mapping of such important immune functions as mixed lymphocyte reaction, graft versus host reactivities, immune response control (It genes), and cell-cell interaction determinants to the I region has stimulated efforts to identify possible relationships between Ia determinants and these immune functions (9)(10)(11)(12)(13)(14).…”

mentioning

confidence: 99%

Effects of anti-Ia sera on mitogenic responses. II. Differential expression of the Ia marker on phytohemagglutinin and concanavalin A-reactive T cells.

Niederhuber¹,

Frelinger²,

Dine³

et al. 1976

Genes mapping in the I region of the H-2 complex control a system of lymphocyte alloantigens (Ia) which are expressed on a subpopulation of T cells and on most B cells. Specific anti-Ia serum in the presence of rabbit complement removed the splenic T-cell subpopulation responsive to Con-A, but did not affect the response to phytohemagglutinin (PHA) or Leucoagglutinin. Antibodies specific for Ia, H-2K, or H-2D membrane antigens were used without complement to pretreat spleen cells. These antibody pretreated cells responded normally to Con-A and PHA.