SEROLOGICAL TYPING OF <i>STAPHYLOCOCCUS AUREUS</i>

Haukenes, Gunnar

doi:10.1111/apm.1964.60.2.285

Cited by 15 publications

(1 citation statement)

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“…The serum soft-agar assay demonstrated production of extracellular polysaccharide in the mastitis strains. Furthermore, the quantitative variation in binding of different S. aureus strains to the K-12-coated Dynabeads in vivo may represent different serological types (5,23), as was also described for human clinical isolates of S. aureus (6,11,13).…”

Section: Discussionmentioning

confidence: 84%

Staphylococcus aureus exopolysaccharide in vivo demonstrated by immunomagnetic separation and electron microscopy

1989

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Staphylococcus aureus strains were separated from mastitis milk samples without cultivation by using monodisperse magnetic polymer particles coated with polyclonal antiserum against an encapsulated S. aureus strain. Exopolysaccharide was verified by transmission electron microscopy and the serum soft-agar culture technique. Capsular polysaccharide was found on virtually all clinical isolates. Surface protein A and S. aureus-specific cell wall components were masked when the strains were cultured on an exopolysaccharide-promoting medium. Masking of surface determinants was dependent on their concentration on the bacterial surface as well as on exopolysaccharide abundance. The polysaccharide layer on in vivo bacteria was reduced markedly after just one transfer from milk to blood agar plates but was reexpressed after culturing was done on a capsule-generating medium.

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Section: Discussionmentioning

confidence: 84%