Serological types of typhus virus and corresponding types of Proteus

Félix, Arthur

doi:10.1016/s0035-9203(33)90005-x

Cited by 28 publications

(4 citation statements)

References 19 publications

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“…The three peaks were pooled, dried, and dissolved in (6) further reported that, according to serological test and the gel filtration, OX2-and OX19-LPS were the rough type and OXK-LPS was the smooth type. We cannot explain the discrepancy on OX2-LPS between ours and theirs, but it is known that Proteus strains may mutate from smooth to rough when stored on agar plants (8).…”

Section: Neutral Sugar Compositions Of Lpscontrasting

confidence: 79%

Chemical Characterization of Lipopolysaccharides from Proteus Strains Used in Weil‐Felix Test

Mizushiri¹,

Amano²,

Fujii³

et al. 1990

Microbiology and Immunology

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The lipopolysaccharides (LPS) extracted from Proteus strains OX2, OX 19, and OXK used as antigens in the Weil-Felix test, were characterized by chemical analysis and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). To separate the O-polysaccharide, core-oligosaccharide, and lipid A moieties, each LPS was treated with 2% acetic acid, centrifuged, and applied to Sephadex G-50 column. The core-oligosaccharides contained L-glycero-D-mannoheptose, D-glycero-D-mannoheptose, glucose (Glc), galactose, 3-deoxy-D-mannooctulosonic acid, uronic acid, phosphate, glucosamine (GlcN), and galactosamine (GalN). The lipid A preparations contained GlcN, GlcN-phosphate, and three fatty acids (myristic, palmitic, and hydroxymyristic acids).However, the O-polysaccharides of OX2-and OXK-LPS had different chemical compositions which consisted of Glc, G1cN, and quinovosamine, and Glc, uronic acid, and GalN, respectively, while OX19-LPS seemed to lack O-polysaccharide.Some strains of Proteus have been used as antigens in the Weil-Felix (WF) test for the diagnosis of rickettsial diseases (21). Whole cells (WC) of Proteus vulgaris OX2 react strongly with the sera from persons infected with spotted fever group rickettsia, except from those infected with Rocky Mountain spotted fever, while the WC of P. vulgaris OX19 react with sera from persons infected with the typhus group as well as with Rocky Mountain spotted fever rickettsia. The WC of Proteus mirabilis OXK react only with sera from persons infected with R. tsutsugamushi (scrub typhus group). Reports concerning the antigenic relationship between Proteus and Rickettsia are limited. Castaneda (5) showed that P. vulgaris OX19 possessed an acid-stable and moderately alkali-stable polysaccharide antigen, which was responsible for the cross-reaction with Rickettsia.Studies of lipopolysaccharides (LPS) from Proteus strains have been done by various workers (for review, see Ref. 12). Dmitriev et al (6) analyzed the polysaccharides from several serotypes of P. vulgaris and P. mirabilis and showed them to contain galactosamine (GalN), galactose (Gal), glucose (Glc), 6-deoxyhexosamine, address:

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Section: Neutral Sugar Compositions Of Lpscontrasting

confidence: 79%

Chemical Characterization of Lipopolysaccharides from Proteus Strains Used in Weil‐Felix Test

Mizushiri¹,

Amano²,

Fujii³

et al. 1990

Microbiology and Immunology

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“…No positive reaction with titers 1 : 80 was found in control sera from 45 healthy adults in nonendemic areas, and only 2% of these specimens showed agglutination at 1 : 40. The results were similar to those described in 1933 by Felix (25), who reached a complete agreement with Durand's observation of WF reactions for boutonneuse fever, i.e., agglutinins were, in some cases, found either solely or predominantly to OX19, in other cases to OX2. Although the results of WF test triggered the investigation of SFG rickettsiosis in Japan, it should be noted that WF tests have less diagnostic value and that the OX19 antigen commercially available in our country still does not agglutinate properly in the positive sera.…”

Section: Reactivity Of Proteus Vulgaris Ox2 and Ox19 With Patientsupporting

confidence: 92%

Rickettsia japonica, the Etiologic Agent of Oriental Spotted Fever

Uchida

1993

Microbiology and Immunology

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“…The well-known cross-reaction between certain Proteus strains and Rickettsia has been used as a presumptive diagnosis of rickettsial infections for many years (8,21). This test, known as the WF test, employs Proteus strains OX2, OX 19, and OXK, which give different agglutination patterns with sera of patients infected with different rickettsial agents.…”

Section: Discussionmentioning

confidence: 99%

Immunological Characterization of Lipopolysaccharides from Proteus Strains Used in Weil‐Felix Test and Reactivity with Patient Sera of Tsutsugamushi Diseases

Amano

Mizushiri

Fujii

et al. 1990

Microbiology and Immunology

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Immunological analyses of lipopolysaccharides (LPS) isolated from Proteus strains OX2, OX19, and OXK used as antigens of Weil‐Felix (WF) test, were performed by quantitative agglutination, enzyme‐linked immunosorbent assay (ELISA), and immunoblotting. Antisera against LPS and whole cells (WC) of the three Proteus strains reacted with homologous LPS but not with heterologous LPS. and the reaction was inhibited by the O‐polysaccharide fraction isolated from the homologous LPS except OX19‐LPS. which lacked O‐polysaccharide moiety. The immunological data support the findings that the O‐polysaccharide moieties of LPS from OX2 and OXK strains possess different chemical composition (Mizushiri, Amano, Fujii, Fukushi, and Watanabe, Microbiol. Immunol. 34: 121 133, 1990). Antisera against Proteus strains reacted weakly with WC of Rickettsia prowazekii, Rickettsia typhi, and Rickettsia tsutsugamushi. Antisera from patients with tsutsugamushi disease reacted with OXK‐WC by WF test when the sera were obtained 13 days after onset of fever. The immunoperoxidase (IP) test titers of these antisera began to rise 6 days after the onset of fever. By ELISA tests these antisera reacted with OXK‐WC and OXK‐LPS independently of the titers of WF or IP tests.

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Serological types of typhus virus and corresponding types of Proteus

Cited by 28 publications

References 19 publications

Chemical Characterization of Lipopolysaccharides from Proteus Strains Used in Weil‐Felix Test

Chemical Characterization of Lipopolysaccharides from Proteus Strains Used in Weil‐Felix Test

Rickettsia japonica, the Etiologic Agent of Oriental Spotted Fever

Immunological Characterization of Lipopolysaccharides from Proteus Strains Used in Weil‐Felix Test and Reactivity with Patient Sera of Tsutsugamushi Diseases

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