Serological Tests in the Diagnosis and Prognosis of Coccidioidompcosis12

Smith, Charles E.; Saito, Margaret T.; Beard, Rodney R.; Kepp, Ruth McFADDEN; Clark, Ruth Wheatlake; Eddie, B.

doi:10.1093/oxfordjournals.aje.a119404

Cited by 120 publications

(151 citation statements)

References 0 publications

Supporting

Mentioning

148

Contrasting

Order By: Relevance

“…The approximately 60% of persons who are without acknowledged symptoms can be detected by a positive skin test with coccidioidal antigen; in some, detection of prior coccidioidal infection results when a pulmonary lesion is detected by routine chest roentgenogram, and subsequent laboratory tests confirm its etiology. Smith et al (139) found only 7% seropositivity in a small sample of asymptomatic skin test converters without evidence of disease.…”

Section: Spectrum Of Diseasementioning

confidence: 96%

“…Smith et al (142) could show no passive transfer (PrausnitzKustner test) of immediate-type hypersensitivity from five individuals with current and prior coccidioidal infections and positive coccidioidin skin tests to coccidioidin-negative recipients. In these studies (142), it was apparent that, in primary nondisseminating coccidioidomycosis, skin reactivity to coccidioidin usually preceded by a few days the formation of early antibody (detected by TP test), which in turn was followed by development of CF antibodies (139,141) (Fig. 2).…”

Section: Allergymentioning

confidence: 99%

“…Over a 15-year period, the serologic studies of Smith et al (139,141) involved some 39,500 tests on patients, including 5,579 with primary nondisseminating coccidioidal infections, 722 with disseminated infections, 271 with noncavitating pulmonary residuals, and 577 with pulmonary cavities. The temporal sequence of antibody detected by TP (Fig.…”

Section: Antibodymentioning

confidence: 99%

See 2 more Smart Citations

Serology of coccidioidomycosis

1990

View full text Add to dashboard Cite

Serologic tests have assisted in the diagnosis and prognosis of coccidioidomycosis for a half-century. The causative agent, Coccidioides immitis, is a dimorphic fungus existing in a hyphal form with arthroconidia in nature and in the usual culture. The arthroconidia represent the inhaled infective forms which in vivo and under special laboratory conditions form spherules which endosporulate. The culture filtrate/autolysate (coccidioidin) from the hyphal phase has provided antigens of suitable reliability for currently used serologic tests. These tests are primarily to determine the two major antibody responses: the early immunoglobulin M (IgM) response is useful in the diagnosis of acute primary coccidioidomycosis. Later, IgG is produced and usually outlasts the IgM, persisting in chronic coccidioidomycosis. The IgM is detectable by tube precipitin, a corresponding immunodiffusion, or latex particle agglutination tests. The pertinent antigen(s) is heat stable and pronase resistant and appears to be largely carbohydrate, mainly mannose with some 3-O-methyl mannose. The IgG detectable in the serum and other body fluids by complement fixation and a corresponding immuno-diffusion is useful in diagnosis, and its quantitation provides an indicator of progression of disease (increasing titer) or regression (decreasing titer). The pertinent antigen appears to be a heat-labile, pronase-sensitive protein which in an unreduced form has a molecular weight of 110,000. A third very useful serologic procedure is the exoantigen test for identification of putative cultures of C. immitis.

show abstract

Section: Spectrum Of Diseasementioning

confidence: 96%

Section: Allergymentioning

confidence: 99%

Section: Antibodymentioning

confidence: 99%

See 1 more Smart Citation

Serology of coccidioidomycosis

1990

View full text Add to dashboard Cite

show abstract

“…These four strains had been isolated from active cases of coccidioidomycosis. They were grown on the asparagine synthetic medium used by Smith and associates (1) and by Schubert and co-workers (4) in their investigations. The cultures were incubated for 6 weeks at room temperature in Roux culture bottles.…”

Section: Methodsmentioning

confidence: 99%

“…HE VALUE of the complement fixation test in suspected and proved cases of coccidioidomycosis has been adequately discussed by Smith and co-workers (1,2 With this problem in view, a preliminary investigation was made to determine whether rabbit antiserum could be used to replace human antiserum as a source of positive control serum.…”

mentioning

confidence: 99%