Serological survey suggests circulation of coronavirus on wild Suina from Argentina, 2014–2017

Winter, Marina; Marfil, María Jimena; Sala, Luciano F. La; Suárez, M.; Maidana, Celia; Rodríguez, Carlos J.; Mesplet, María; Abate, S; Rosas, Carolina; Martinez, Jorge Peña; Barandiarán, Soledad

doi:10.1007/s10393-022-01591-x

Cited by 3 publications

(5 citation statements)

References 20 publications

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“…Global data on TGEV and PRCV Ab-prevalence is limited, and the results are mostly shown as negative or show low seroprevalence rates in both domestic pigs and wild boars, as described in Argentina, Slovenia, Finland, the Czech Republic, Germany, Croatia and Turkey [ 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. Exceptions have been described in South Korea, where a PRCV seroprevalence of 53.1% was described in 1999, and in Hungary, where a seroprevalence of 15.42% against TGEV was reported in 2019, even though most of the animals proved to be positive for PRCV [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

A Serological Investigation of Porcine Reproductive and Respiratory Syndrome and Three Coronaviruses in the Campania Region, Southern Italy

Ferrara

D’Anza

Rossi

et al. 2023

Viruses

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Porcine coronaviruses and reproductive and respiratory syndrome (PRRS) are responsible for severe outbreaks that cause huge economic losses worldwide. In Italy, three coronaviruses have been reported historically: porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV). Although repeated outbreaks have been described, especially in northern Italy, where intensive pig farming is common, there is a worrying lack of information on the spread of these pathogens in Europe. In this work, we determined the seroprevalence of three porcine coronaviruses and PRRSV in the Campania region, southern Italy. A total of 443 samples were tested for the presence of antibodies against porcine coronaviruses and PRRSV using four different commercial ELISAs. Our results indicated that PEDV is the most prevalent among porcine coronaviruses, followed by TGEV, and finally PRCV. PRRSV appeared to be the most prevalent virus (16.7%). For coronaviruses, seroprevalence was higher in pigs raised in intensive farming systems. In terms of distribution, TGEV is more widespread in the province of Avellino, while PEDV and PRRSV are more prevalent in the province of Naples, emphasizing the epidemic nature of both infections. Interestingly, TGEV-positive animals are more common among growers, while seropositivity for PEDV and PRRSV was higher in adults. Our research provides new insights into the spread of swine coronaviruses and PRRSV in southern Italy, as well as a warning about the need for viral surveillance.

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Section: Discussionmentioning

confidence: 99%

A Serological Investigation of Porcine Reproductive and Respiratory Syndrome and Three Coronaviruses in the Campania Region, Southern Italy

Ferrara

D’Anza

Rossi

et al. 2023

Viruses

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“…Overall, the TGEV seroprevalence was higher in pigs raised in intensive farming systems, and TGEV appeared to be more widespread in the province of Avellino [ 50 ]. Investigations on the circulation of TGEV and PRCV in Argentina were conducted during 2014–2017 [ 51 ]. Among 87 collard peccary samples, 3/87 samples were positive for TGEV, while PRCV antibodies could not be detected.…”

Section: Discussionmentioning

confidence: 99%

“…Among 87 collard peccary samples, 3/87 samples were positive for TGEV, while PRCV antibodies could not be detected. Furthermore, TGEV or PRCV antibodies could not be found in serum samples collected from 160 wild boars [ 51 ]. In a Spanish study, the diversity of respiratory viruses in nasal swabs for respiratory disease cases in weaned pigs with suspected influenza A (IAV) infection was investigated [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Porcine Respiratory Coronavirus (PRCV): Isolation and Characterization of a Variant PRCV from USA Pigs

Rawal,

Yim-im,

Aljets

et al. 2023

Pathogens

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Porcine respiratory coronavirus (PRCV), a mutant of the transmissible gastroenteritis virus (TGEV), was first reported in Belgium in 1984. PRCV typically replicates and induces mild lesions in the respiratory tract, distinct from the enteric tropism of TGEV. In the past 30 years, PRCV has rarely been studied, and most cited information is on traditional isolates obtained during the 1980s and 1990s. Little is known about the genetic makeup and pathogenicity of recent PRCV isolates. The objective of this study was to obtain a contemporary PRCV isolate from US pigs for genetic characterization. In total, 1245 lung homogenate samples from pigs in various US states were tested via real-time PCR targeting PRCV and TGEV RNA. Overall, PRCV RNA was detected in five samples, and a single isolate (ISU20-92330) was successfully cultured and sequenced for its full-length genome. The isolate clustered with a new group of variant TGEVs and differed in various genomic regions compared to traditional PRCV isolates. Pathogens, such as PRCV, commonly circulate in pig herds without causing major disease. There may be value in tracking genomic changes and regularly updating the diagnostic methods for such viruses to be better prepared for the emergence of variants in ecology and pathogenicity.

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“…PEDV is distributed in many countries and regions including the United States, Europe and Asia, but PEDV in China exhibits higher recombination and evolution rates with increased risks of cross-species transmission [ 4 , 5 ]. Recent epidemiological studies in southern Italy reveal variability in PEDV epidemiology and transmission risks between different animal populations [ 6 , 7 ]. In domestically raised pigs in intensive farming systems, PEDV seroprevalence was much higher than in wild boars in the same region, reaching 14.8%, and showed an increasing trend in adult pigs [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recent epidemiological studies in southern Italy reveal variability in PEDV epidemiology and transmission risks between different animal populations [ 6 , 7 ]. In domestically raised pigs in intensive farming systems, PEDV seroprevalence was much higher than in wild boars in the same region, reaching 14.8%, and showed an increasing trend in adult pigs [ 7 , 8 ]. This poses challenges for research on how to control the spread of this infectious disease.…”

Section: Introductionmentioning

confidence: 99%

RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating Ferroptosis

Li,

Bao,

et al. 2023

Viruses

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Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that induces diarrhea and death in neonatal piglets, resulting in substantial economic losses to the global swine industry. The mechanisms of PEDV infection and the roles of host factors are still under exploration. In this study, we used the ferroptosis pathway downstream target activator (1S,3R)-RSL3 compound as a starting point, combined with the interactions of N-acetylcysteine and deferoxamine, to elucidate the effects of a series of compounds on PEDV proliferation. We also established glutathione peroxidase 4 (GPX4) gene overexpression to further elucidate the relationship between the ferroptosis pathway and PEDV. (1S,3R)-RSL3 inhibited PEDV replication in Vero cells, while N-acetylcysteine and deferoxamine promoted its proliferation. In addition, (1S,3R)-RSL3 mainly affected the replication stage of PEDV. Overexpression of GPX4 promoted PEDV proliferation, indicating that the ferroptosis pathway could influence PEDV replication in Vero cells. This study focused on the mechanism of (1S,3R)-RSL3 inhibition on PEDV, laying the foundation for exploring the pathogenic mechanisms of PEDV and drug development.

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Serological survey suggests circulation of coronavirus on wild Suina from Argentina, 2014–2017

Cited by 3 publications

References 20 publications

A Serological Investigation of Porcine Reproductive and Respiratory Syndrome and Three Coronaviruses in the Campania Region, Southern Italy

A Serological Investigation of Porcine Reproductive and Respiratory Syndrome and Three Coronaviruses in the Campania Region, Southern Italy

Porcine Respiratory Coronavirus (PRCV): Isolation and Characterization of a Variant PRCV from USA Pigs

RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating Ferroptosis

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