Serological survey of Leishmania infection in dogs from the municipality of Peso da Régua (Alto Douro, Portugal) using the direct agglutination test (DAT) and fast agglutination screening test (FAST)

Cardoso, Luı́s; Schallig, Henk D. F. H.; Neto, Francisco; Kroon, Nel C. M.; Rodrigues, M.A.M.

doi:10.1016/j.actatropica.2004.03.004

Cited by 33 publications

(22 citation statements)

References 20 publications

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“…The assay had been previously employed for serum diagnosis of canine infection by Leishmania in animals with asymptomatic visceral leishmaniasis (36,37). In the current study, the authors are reporting the presence of anti-Leishmania antibodies in Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Procyon cancrivorus and Nasua nasua.…”

Section: Search For Antibodies By Datmentioning

confidence: 64%

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Voltarelli

Arraes

Perles

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

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Leishmania sp. infection was investigated in wild animals from the Ingá Park, in the municipality of Maringá, Paraná state, Brazil, where American cutaneous leishmaniasis (ACL) is an endemic disease. Sixty-five mammals, comprising Didelphis albiventris, Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Dasypus novemcinctus, Procyon cancrivorus and Nasua nasua, were captured. Blood samples were collected for parasite cultivation. Antibodies were investigated by direct agglutination test (DAT) using Leishmania (Viannia) braziliensis as antigen. Flagellates were observed in blood cultures of 14 (35.9%) Didelphis albiventris. Anti-Leishmania antibodies were detected in 31 (51.6%) specimens of Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Procyon cancrivorus and Nasua nasua. These results suggest that Cerdocyon thous and Lycalopex vetulus (crab-eating fox), Cebus apella (capuchin monkey), Dasyprocta azarae (agouti), Procyon cancrivorus (crab-eating raccoon) and Nasua nasua (coati) play an important role in the ACL transmission cycle in the northwestern region of Paraná, Brazil.

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Section: Search For Antibodies By Datmentioning

confidence: 64%

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Voltarelli

Arraes

Perles

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

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“…The presence of anti-Leishmania-specific antibodies in asymptomatic, oligosymptomatic, and symptomatic infected dogs (5,12,45) has allowed the development of serologic tests, including immunofluorescent antibody tests, Western blotting, immunochromatographic tests, and enzyme-linked immunosorbent assays (ELISAs) (for a review, see reference 29). The diagnosis of CVL by ELISAs based on crude soluble Leishmania antigens (SLAs) have shown a high degree of sensitivity but a low degree of specificity because of the antigenic relatedness of Leishmania and other pathogenic protozoa (21).…”

mentioning

confidence: 99%

Specific Serodiagnosis of Canine Visceral Leishmaniasis Using Leishmania Species Ribosomal Protein Extracts

Coelho

Ramírez

Costa³

et al. 2009

Clin Vaccine Immunol

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In the present work, we have analyzed the antigenicity of Leishmania species ribosomal proteins (LRPs). To accomplish this, Leishmania infantum ribosomes were biochemically purified from promastigote cytosolic extracts, and their reactivities were analyzed by using the sera from dogs naturally infected with L. infantum. Since antibodies reacting against different ribosomal proteins were observed in all the serum samples obtained from dogs with symptomatic visceral leishmaniasis tested, we have analyzed the potential usefulness of the LRP extracts in the development of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of canine visceral leishmaniasis (CVL) in an area of Brazil where visceral leishmaniasis is endemic due to infection by Leishmania chagasi. A comparative ELISA with crude soluble Leishmania chagasi antigen (SLA) and L. infantum LRPs was performed. LRP-and SLA-based ELISAs gave similar sensitivities for the diagnosis of symptomatic CVL, but the LRP extract provided a very high sensitivity for the detection of oligosymptomatic and asymptomatic dogs. In addition, an LRP-based ELISA showed a higher specificity when the sera from dogs harboring other infections were included in the analysis. The LRP antigen displayed no cross-reactivity with sera from dogs that had any of the other diseases tested, notably, Chagas' disease. Our findings suggest that LRPs are a potential tool for the diagnosis of CVL and will be particularly useful for the diagnosis of asymptomatic CVL.

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“…These results credit FAST as a qualitative preliminary screening test considering high sensitivity rate and DAT as a quantitative confirmatory test especially in areas of high sero-prevalence. The same results were achieved by Cardoso et al in Portugal in 2004(Cardoso et al 2004), but in this study, we used from the batches of DAT and FAST antigens that were prepared in our lab from Iran .With FAST, the final data often could be available in the same day after 3 h because it is not necessary to re-test those sero-negative samples with DAT. We only re-tested FAST-positive sera by DAT for confirmation, and results were available the next day.…”

Section: Discussionmentioning

confidence: 65%

“…DAT has been proven to be a very important serodiagnostic tool combining high validity and easy to performance (Harith et al 1989;Edrissian et al 1996;Mohebali et al 2006). Otherwise, FAST can perform with only one serum dilution and making the test very suitable for the screening of VL in large populations of humans and dogs (Cardoso et al 2004). …”

Section: Discussionmentioning

confidence: 99%

Rapid detection of Leishmania infantum infection in dogs: a comparative study using fast agglutination screening test (FAST) and direct agglutination test (DAT) in Iran

et al. 2009

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Canine visceral leishmaniasis (CVL) is not only a veterinary problem but has also a serious public health importance. Rapid detection of CVL is highly important for control of human visceral leishmaniasis in Iran. This study was aimed to compare the fast agglutination screening test (FAST) with direct agglutination test (DAT) as a standard serological test for the detection of anti-Leishmania antibodies on dog serum samples. DAT and FAST antigens were prepared in the School of Public Health, Tehran University of Medical Science. Altogether, 73 serum samples from Leishmania infantum infection dogs and 74 sera from healthy controls were collected from human VL/CVL endemic and non-endemic areas of Iran, respectively. All the sera were evaluated with both FAST and DAT techniques. A sensitivity of 98.60% (95% CI, 98.57-98.62) and specificity of 78.70% (95 CI%, 69.20-88.20) were found at a 1:160--(cut-off) titer when DAT confirmed cases were compared with healthy control. A good degree of agreement was observed between FAST and DAT (86.8%) by kappa analysis (p < 0.01). In conclusion, this study showed that FAST is very practical and simple diagnostic tool for the sero-diagnosis of CVL in endemic areas of Iran.

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Serological survey of Leishmania infection in dogs from the municipality of Peso da Régua (Alto Douro, Portugal) using the direct agglutination test (DAT) and fast agglutination screening test (FAST)

Cited by 33 publications

References 20 publications

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Specific Serodiagnosis of Canine Visceral Leishmaniasis Using Leishmania Species Ribosomal Protein Extracts

Rapid detection of Leishmania infantum infection in dogs: a comparative study using fast agglutination screening test (FAST) and direct agglutination test (DAT) in Iran

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