Serological studies with respiratory syncytial virus

Doggett, Jennifer E.; Taylor‐Robinson, David

doi:10.1007/bf01245207

Cited by 36 publications

(18 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our earlier study differences in neutralizing antibody titres had been detected by the use of a strain of RS virus isolated locally but we could not repeat this finding with strains isolated during the present study. This discrepancy could be explained by differences of 'avidity' or antigenic variation between the three local strains used; work is in progress to elucidate this point for although antigenic differences have been found between RS virus strains these have not been detectable in neutralization tests with human sera (Coates, Kendrick & Chanock, 1963;Doggett & Taylor-Robinson, 1965). The value of unheated rabbit serum in the measurement of neutralizing antibodies against rubella virus has been reported by several workers (Neva & Weller, 1964;Plotkin, 1964;Parkman, Mundon, McCown & Buescher, 1964) Infections with respiratory syncytial (RS) virus, parainfluenza virus and herpes simplex virus respectively were found in 29, 11 and 12 children in the respiratory illness group but in only 1, 2 and 4 children in the diarrhoeal group.…”

Section: Discussionmentioning

confidence: 99%

A comparative virological study of children in hospital with respiratory and diarrhoeal illnesses

Stott

Bell

Eadie

et al. 1967

J. Hyg.

View full text Add to dashboard Cite

Between October 1963 and April 1965, 113 children with respiratory disease and 113 children with diarrhoeal disease were matched for age and time of entry into hospital and studied by virus isolation and serological techniques.Infections with respiratory syncytial (RS) virus, parainfluenza virus and herpes simplex virus respectively were found in 29, 11 and 12 children in the respiratory illness group but in only 1, 2 and 4 children in the diarrhoeal group. Rhinoviruses were isolated from 10 children in each group and in seven cases were associated with lower respiratory disease. Adenovirus infections were found in nine children with respiratory disease and eight with diarrhoea. Of the 40 enteroviruses isolated 16 were associated with respiratory disease and 24 with diarrhoea.A poor or delayed serological response in children under 4 months with RS virus infection was observed. Addition of unheated rabbit serum increased the sensitivity of the neutralization test with RS virus.These findings indicate that respiratory syncytial and parainfluenza virus infections were clearly associated with respiratory illness but the pathogenic role of the other viruses was not clear.

show abstract

Section: Discussionmentioning

confidence: 99%

A comparative virological study of children in hospital with respiratory and diarrhoeal illnesses

Stott

Bell

Eadie

et al. 1967

J. Hyg.

View full text Add to dashboard Cite

show abstract

“…The subtype A strains included the prototype laboratory strains A2 and Long, the strain CH287 which was evaluated as a parenterally administered live vaccine (Belshe et al, 1982), the strains WV9894 and WV12138 characterized in a previous study (Mufson et al, 1985) and three strains (V214, V316, V401) isolated in Bristol, U.K. between 1983 and 1984 and provided by Dr E. J. Stott, Institute for Research on Animal Diseases, Compton, Newbury, U.K. The subtype B strains included two strains isolated during the early 1960s, CH18537 (Coates et al, 1963) obtained from Dr R. M. Chanock, NIAID, National Institutes of Health, Bethesda, Md., U.S.A. and a Swedish strain, 8/60 (Doggett & Taylor-Robinson, 1964), obtained from Dr E. J. Stott (both have been defined as B subtypes; E. Norrby, unpublished), the strains WV1293, WV3212, WV4843 and WV6873 characterized previously (Mufson et al, 1985) and two strains (V422 and V463) isolated in Bristol between 1983 and 1984 and provided by Dr E. J. Stott. The LEC strain of measles virus (see Sheshberadaran et al, 1983) was used as a control in experiments with tunicamycin-treated cells.…”

Section: Methodsmentioning

confidence: 99%

“…However, it appears that the involvement of RS virus F protein is relatively more important both for neutralization phenomena in vitro and for events restricting replication in vivo. It remains to be determined whether the difference between the homologous and heterologous neutralization titres of strain-specific polyclonal hyperimmune sera (Coates et al, 1963;Wulff et al, 1964, Doggett & Taylor-Robinson, 1964) which exemplify differences between subtype A and B strains (E. Norrby, unpublished data) reflect antigenic differences between G or F components. Since it is more likely that the F antigen is involved, the finding in this study of distinct differences between the F components of subtypes A and B assume a special significance.…”

Section: E N O R R B Y M a M U F S O N A N D H S H E S H B E R mentioning

confidence: 99%

“…Although cross-neutralization tests with animal hyperimmune sera demonstrated differences between isolates of the virus (Coates et al, 1963; Wulffet al, 1964;Doggett & Taylor-Robinson, 1964) no corresponding differences were observed with infant sera and they were therefore not considered to be of any relevance. With the advent of monoclonal antibodies (MAbs) the antigenic stability of RS virus could be assessed again.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structural Differences between Subtype A and B Strains of Respiratory Syncytial Virus

Norrby

Mufson

Sheshberadaran

1986

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYDifferences in the properties of homologous intracellular structural components of eight strains of subtype A and eight strains of subtype B of human respiratory syncytial (RS) virus were examined. The size of the fusion (F) protein cleavage products and the phosphoprotein (P) showed systematic differences between virus strains representing the two subtypes. The apparent mol. wt. in SDS-polyacrylamide gels under reducing conditions was 48000 (48K) and 46K to 47K for the cleavage product FI in subtype A and B strains, respectively. The size of the F2 protein was 18K to 20K. The subtype B strains showed a slightly higher tool. wt. of this protein compared to the subtype A strains. The size of the P protein was 36K in subtype A strains, but only 34K in subtype B strains. Variations also occurred in the size of the glycoprotein (G) and the 22K to 24K structural protein. These variations did not correlate with the virus subtypes, but were strain-specific. The size of non-glycosylated forms of the F protein cleavage products was determined by use of material from tunicamycin-treated cells. A 44K to 45K non-glycosylated form of the F 1 protein was detected with subtype A virus strains, but the corresponding protein of subtype B strains was not reproducibly identified, presumably due to instability in the absence of glycosylation or altered antigenicity. Monoclonal antibody immunosorbent-bound viral glycoproteins were partially digested with proteases. The pattern of breakdown products of the F1 protein was distinctly different between subtype A and B strains, but it was similar among strains of the same subtype. No subtype-specific pattern was seen in proteolytic digests of monoclonal antibody-bound G protein. INTRODUCTIONFor the 3 decades since its discovery human respiratory syncytial (RS) virus has been considered to represent a single homogeneous serotype. Although cross-neutralization tests with animal hyperimmune sera demonstrated differences between isolates of the virus (Coates et al., 1963; Wulffet al., 1964;Doggett & Taylor-Robinson, 1964) no corresponding differences were observed with infant sera and they were therefore not considered to be of any relevance. With the advent of monoclonal antibodies (MAbs) the antigenic stability of RS virus could be assessed again. Studies with such reagents revealed that isolates of RS virus exhibited variations in epitope occurrence (Gimenez et al., 1984) but that these variations were more extensive than could be explained by incidental mutational changes (Anderson et al., 1985;Mufson et al., 1985). The isolates could be separated into two categories based on their characteristic reaction pattern with different MAbs. The two groups were named RS virus subtype A and B (Mufson et al., 1985). These subtypes showed different properties in at least four different structural proteins emphasizing that they had become ecologically adjusted after a period of separate evolution. Characterization of the epidemiological occurrence of the two RS virus subtypes

show abstract

“…Early studies of RS virus that used polyclonal animal antisera to characterize numerous isolates found that most were indistinguishable, although occasional variants were detected (3)(4)(5)(6)(7). In the years after the discovery of the virus, numerous studies were carried out which elucidated the epidemiology of RS virus (2, 6,[8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

RNA fingerprinting of respiratory syncytial virus using ribonuclease protection. Application to molecular epidemiology.

Storch¹,

Park²,

Dohner³

1989

J. Clin. Invest.

View full text Add to dashboard Cite

We have used the technique of ribonuclease protection to define genomic variation among circulating isolates of subgroup A respiratory syncytial (RS) virus. RNAs extracted from HEp-2 cells infected with strains to be analyzed were hybridized with a 32P-labeled RNA probe corresponding to the RS virus G glycoprotein (A2 strain). Areas of nonhomology were detected by cleavage with ribonuclease A. Using this technique, multiple distinct RNA cleavage patterns could be distinguished among viral isolates recovered from infants residing in the same metropolitan area and infected during the same epidemic season. Epidemiologically related isolates (from coinfected twins, from infants infected during a nosocomial outbreak at an extended care facility, and from institutionalized adults infected during an outbreak) yielded identical patterns. In two separate outbreaks, differences in cleavage patterns among certain isolates corresponded to epidemiologically significant differences among the individuals from whom the isolates were recovered. We conclude that substantial genomic heterogeneity exists among circulating isolates of subgroup A RS virus. Ribonuclease protection can be used as a molecular fingerprinting tool for expanded studies ofthe molecular epidemiology of this virus.

show abstract

Serological studies with respiratory syncytial virus

Cited by 36 publications

References 9 publications

A comparative virological study of children in hospital with respiratory and diarrhoeal illnesses

A comparative virological study of children in hospital with respiratory and diarrhoeal illnesses

Structural Differences between Subtype A and B Strains of Respiratory Syncytial Virus

RNA fingerprinting of respiratory syncytial virus using ribonuclease protection. Application to molecular epidemiology.

Contact Info

Product

Resources

About