Serological response of cattle after vaccination and challenge with Brucella abortus

Sutherland, S. S.; Cras, D. V.; Robertson, A.; Johnston, J. M.; Evans, Richard J.

doi:10.1016/0378-1135(82)90028-1

Cited by 23 publications

(9 citation statements)

References 12 publications

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“…Any female calf vaccinated 30 to 50 days before the sampling date with the commercial brucellosis vaccine approved by SENASA (2 ml-dose) was expected to react positively to the DT (DT+ = Buffered Plate Antigen Test + and/or Complement Fixation Test +) [ 7 , 8 ]. We classified a farm as “well vaccinated” if the proportion of DT+ was not significantly lower than the ideal 95%.…”

Section: Methodsmentioning

confidence: 99%

Methodology for the assessment of brucellosis management practices and its vaccination campaign: example in two Argentine districts

Aznar

Arregui²,

Humblet

et al. 2017

BMC Vet Res

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BackgroundIn Argentina, vaccination with Brucella abortus Strain 19 vaccine is mandatory. The objective of the study was to develop and test a method for evaluating, in an innovative way, some farmers’ and veterinarians’ management practices in relation to brucellosis and to assess the vaccination campaign and coverage. The work took place in Brandsen and Navarro districts. Four questionnaires were designed (for officials from Local Sanitary Entities, vaccinators, vet practitioners and farmers). Responses were coded as “ideal” (0) and “not ideal” (1). To assess the relative weight of each question (“item”), experts ranked the items according to their impact on management practices and vaccination. A weighted score was then calculated. A higher weighted score was assigned to the worse practices. Farmers obtaining a global weighted score above the third quartile were classified as “inappropriately managed farms”, to be compared per type of production system and district. To assess the immunization coverage, female calves were sampled 30 to 50 days post vaccination; they were expected to react positively to serological diagnostic tests (DT+).ResultsThere were significantly more inappropriately managed farms and higher global scores among beef farmers and in Brandsen. Eighty three percent (83%) of female calves were DT+, significantly under the ideal immunization coverage (95%). Only 48% of farms were considered well vaccinated. DT+ results were positively associated with the Brandsen district (OR = 25.94 [4.60–1146.21] and with the farms having more than 200 cow heads ((OR = 78.34 [4.09–1500.00]). On the contrary, DT+ were less associated with vaccinators being veterinary practitioners (OR = 0.07 [0.006–0.78]). Farmers are well advised by their veterinary practitioners but they should improve some management practices.ConclusionsThe vaccination campaign is globally well implemented, but the immunization coverage and some vaccinators’ practices should be improved.This study leads to a better understanding of the most common used management and control practices regarding brucellosis, which affect its epidemiology. Any vaccination campaign should be periodically assessed to highlight possible fails. The described methodology can be extrapolated to other countries and different contexts.

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Section: Methodsmentioning

confidence: 99%

Methodology for the assessment of brucellosis management practices and its vaccination campaign: example in two Argentine districts

Aznar

Arregui²,

Humblet

et al. 2017

BMC Vet Res

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“…Além disso, apresenta um relevante histórico de serviços prestados no combate à brucelose animal, uma vez que a maioria dos países desenvolvidos que conseguiram erradicar a brucelose atingiram esse êxito usando a reação de fixação de complemento como prova confirmatória. Certamente contribuiu para isso o fato de a prova apresentar boa correlação com o isolamento de Brucella abortus de bovinos infectados (Hayes & Chappel 1982, Sutherland et al 1982, Corner et al 1983.…”

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Estabilidade do antígeno de célula total de Brucella abortus para uso no diagnóstico sorológico da brucelose bovina pela reação de fixação de complemento

Mathias¹,

Meirelles²,

Buchala³

2007

Pesq. Vet. Bras.

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A reação de fixação de complemento é um dos testes usados no diagnóstico confirmatório da brucelose bovina, e para sua realização emprega-se o mesmo antígeno usado na prova de soroaglutinação lenta, porém não foi possível encontrar na literatura estudos sobre a estabilidade desse antígeno para uso na prova de fixação de complemento, de modo a estabelecer um prazo de validade para o mesmo. Por isso, esta investigação teve por objetivo avaliar a estabilidade do antígeno de célula total de Brucella conservado sob refrigeração, para uso na reação de fixação de complemento. Analisaram-se 14 partidas de antígeno, preparado com Brucella abortus amostra 1119/3 e padronizado para uso na prova de soroaglutinação lenta, com tempo de fabricação variando de 9 meses a 23 anos e 11 meses. Testaram-se 167 soros bovinos com títulos variáveis de anticorpos contra Brucella, adotando-se a técnica com incubação a 37ºC nas duas fases da reação e 5 unidades hemolíticas 50% de complemento. Considerou-se como positivo o soro com pelo menos 25% de fixação de complemento na diluição 1:4. Compararam-se os resultados obtidos com as 13 partidas de antígeno com aqueles obtidos com a partida com 9 meses de fabricação, usando o teste de chi2 de McNemar e o coeficiente kappa. A grande maioria dos soros apresentou resultados muito próximos quando testados com as diversas partidas de antígeno, e não se observou relação entre tempo de fabricação do antígeno e diferenças nos resultados obtidos.

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“…Second, some infected cattle may show aberrant serological reactions and are not detected (7,15). Third, maternally infected cattle may be difficult to differentiate from those recently vaccinated with either of the above vaccines (8,16). Finally, there are variations in the test due to differences in technique (1).…”

mentioning

confidence: 99%

Comparison of enzyme-linked immunosorbent assay and complement fixation test for the detection of specific antibody in cattle vaccinated and challenged with Brucella abortus

Sutherland¹

1985

J Clin Microbiol

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An enzyme-linked immunosorbent assay (ELISA) and a complement fixation test (CFT) were applied to sera collected regularly from 60 cattle challenged with Brucella abortus strain 544. Of the 60 cattle, 48 were vaccinated with either B. abortus strain 19 or B. abortus strain 45/20 as calves or adults. The remaining 12 cattle were not vaccinated. Of the 27 sera from cattle found to be infected at slaughter, 9 showed aberrant reactions to the CFT in that a positive titer after challenge was delayed or transient. The performance of the ELISA in these nine cattle with aberrant reactions and the other cattle in the trial was as follows. After vaccination with strain 19 or strain 45/20, the number of weeks at which the ELISA was positive was significantly greater (P < 0.001) than that for the CFT. The strain 19 vaccine also induced positive responses to the CFT and the ELISA for a significantly longer period (P < 0.001) than did the strain 45/20 vaccine. For cattle with aberrant reactions, the number of weeks after challenge when the ELISA was positive was significantly greater (P < 0.001) than that for the CFT. For noninfected cattle, the average number of weeks after challenge when the CFT was negative (35 of 40) was higher than that for the ELISA (18 of 40).

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Serological response of cattle after vaccination and challenge with Brucella abortus

Cited by 23 publications

References 12 publications

Methodology for the assessment of brucellosis management practices and its vaccination campaign: example in two Argentine districts

Methodology for the assessment of brucellosis management practices and its vaccination campaign: example in two Argentine districts

Estabilidade do antígeno de célula total de Brucella abortus para uso no diagnóstico sorológico da brucelose bovina pela reação de fixação de complemento

Comparison of enzyme-linked immunosorbent assay and complement fixation test for the detection of specific antibody in cattle vaccinated and challenged with Brucella abortus

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