Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization

Thouless, Margaret E.; Bryden, A.S.; Flewett, T. H.; Woode, G. N.; Bridger, Janice C.; Snodgrass, D. R.; Herring, J. A.

doi:10.1007/bf01315627

Cited by 135 publications

(82 citation statements)

References 21 publications

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“…The results obtained indicated that antiserum prepared against the Lincoln strain of bovine rotavirus showed various neutralization patterns for the isolates, and these results were different from those of the CF test considered to be related with common "group antigen" of rotaviruses (5,10,12). They suggest that there may be serotypes in bovine rotaviruses by the SN test, as in human and avian rotaviruses (2,5,11,13).…”

contrasting

confidence: 38%

Neutralizing Patterns of Anti‐Bovine Rotavirus (Lincoln) Serum against Cytopathic Bovine Rotaviruses Isolated from Calves in Japan

Murakami

Nishioka

Hashiguchi

et al. 1981

Microbiology and Immunology

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Recent studies on rotavirus have shown that there are several serotypes in human and avian rotaviruses discriminated by serum neutralization (SN) tests (2,5, 11,13). Although considerable attention has been paid to proving the existence of rotaviral serotypes in other species (4), comparatively little is known about those of bovine rotaviruses. There is only one report that distinguished two bovine rotavirus strains by the hemagglutination inhibition (HI) test (9). Since bovine rotavirus strains do not always possess hemagglutinating properties (3, 9), it is difficult to distinguish serotypes in them by the HI test.The present report gives the results of SN and complement fixation (CF) tests with antiserum against Lincoln strain of bovine rotavirus when these tests were applied to the cytopathic bovine rotaviruses isolated from the feces of diarrheic calves in Japan.The Lincoln strain at the 17th passage in bovine kidney cells (7) was kindly supplied by Dr. M. Kodama, of the authors' institute. It was further passaged three times in a continuous line of fetal rhesus monkey kidney (MA 104) cells in the presence of trypsin (Type 1, Sigma Chemical Co.) at the authors' laboratory.Isolation and electron microscopic detection of rotaviruses were performed in essentially the same manner as described by Babiuk et al (1). Briefly, the saline extracts of feces obtained from diarrheic calves were treated with 10 ,ug/ml of trypsin at 37 C for 30 min and inoculated into eight tube cultures of MA 104 cells washed with Earle's solution, in 0.1 ml of a ten-fold serial dilution. Incubation was carried out in the presence of 1 ,ug/ml of trypsin. The tube culture with intense cytopathic effect (CPE) at the end of the serial dilution was used for more than three passages by the same method as described above. Fourteen cytopathic bovine rotaviruses were isolated from different calves of seven herds suffering from diarrhea, and identified by the CF test and electron microscopy (EM). The propagation of MA 104 cells was performed in the manner previously described (6). Viral titration and the SN test were also carried out with MA 104 cells. For the titration, 0.1 ml of ten-fold serial dilution of each virus was inoculated into four tube cultures washed with Earle's solution. After viral adsorption at 37 C for 1097

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confidence: 38%

Neutralizing Patterns of Anti‐Bovine Rotavirus (Lincoln) Serum against Cytopathic Bovine Rotaviruses Isolated from Calves in Japan

Murakami

Nishioka

Hashiguchi

et al. 1981

Microbiology and Immunology

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“…similar morphology (Flewett, Bryden & Davies, 1973;McNulty, 1978) and clinical signs (Flewett & Woode, 1978), share a common group specific antigen (Woode et al 1976; Thouless et al 1977), cross protection (Gaul et al 1982) and inter-species transmissions (Tzipore & Makin, 1978;Dagenais et al 1981). However, despite these findings which indicate that not all rotaviruses are species-specific, there is no direct evidence of rotavirus infection between animal species and man as a zoonosis.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of rotavirus from feral pigeon in mammalian cell cultures

Minamoto¹,

Oki²,

Tomita³

et al. 1988

Epidemiol. Infect.

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SUMMARYAvian rotaviruses were isolated from feral pigeon faeces treated with trypsin using roller tube cultures of mammalian cells. Two pigeon strains, designated as strains PO-8 and PO-13, produced a marked cytopathic effect (CPE), small intracytoplasmic inclusion bodies and high titres of infectious particles in infected MA-104 and MDBK cell lines without cell adaptation and roller drum apparatus. The pigeon rotaviruses shared a common group specific antigen with the Lincoln strain of bovine rotavirus by indirect immunofluorescence, but differed from both the Lincoln strain and the Wa strain of human rotavirus in neutralization tests.

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“…Several subgroups Greenberg et al 1983) and serotypes (Beards et al 1980;Wyatt et al 1982) have been identified by complement fixation tests (Thouless et al 1977;Zissis & Lambert, 1978), ELISA techniques (Yolken et al 1978;Thouless, Beards & Flewett, 1982) and neutralization assays (Beards et al 1980;Wyatt et al 1982).…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Follett

Sanders

Beards

et al. 1984

J. Hyg.

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SUMMARYThe molecular epidemiology of rotavirus infections in Glasgow and the west of Scotland during 1981/82 and 1982/83 was investigated by electron microscopy, ELISA testing and RNA migration pattern analysis. In 1981/82, rotaviruses of both the 'long' and the 'short 'electropherotype (in different variants) co-circulated from the onset throughout the winter peak of the outbreak. Approximately 80 o of the children were infected during the first year of life. No differences in incidence were found between sexes. In 1982/83 the isolated rotaviruses were almost exclusively of the 'long' electropherotype (in different variants) and 360 of the children were infected beyond the first year of life. Rotaviruses of the 'long' electropherotype serologically were of subgroup ii and serotype 1 and those of the 'short' electropherotype of subgroup i and serotype 2.

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Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization

Cited by 135 publications

References 21 publications

Neutralizing Patterns of Anti‐Bovine Rotavirus (Lincoln) Serum against Cytopathic Bovine Rotaviruses Isolated from Calves in Japan

Neutralizing Patterns of Anti‐Bovine Rotavirus (Lincoln) Serum against Cytopathic Bovine Rotaviruses Isolated from Calves in Japan

Isolation and characterization of rotavirus from feral pigeon in mammalian cell cultures

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

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