Serological reactivity of some monoclonal antibodies to varicella-zoster virus

Forghani, Bagher; Schmidt, Nathalie J.; Myoraku, Carolyn K.; Gallo, D

doi:10.1007/bf01318084

Cited by 22 publications

(13 citation statements)

References 11 publications

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“…However, those antigens critical to inducing protection against primary infection or reactivation are not well resolved. Recently, several laboratories reported the production of monoclonal antibodies against VZV virions (10,14,20). Differences in nomenclature have left unclear the actual number and type of glycoprotein antigens in VZV as well as those recognized by these monoclonal antibodies or polyclonal antisera.…”

mentioning

confidence: 99%

Three major glycoprotein genes of varicella-zoster virus whose products have neutralization epitopes

Keller¹,

Neff²,

Ellis³

1984

J Virol

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Varicella-zoster virus (VZV) codes for approximately eight glycosylated polypeptides in infected cell cultures and in virions. To determine the number of serologically distinct glycoprotein gene products encoded by VZV, we have developed murine monoclonal antibodies to purified virions. Of 10 monoclonal antibodies which can immunoprecipitate intracellular VZV antigens and virion glycoproteins, 1 (termed gA) reacted with gp105, 1 (termed gB) reacted with gp115 (intracellular only), gp62, and gp57, and 8 (termed gC) reacted with gp92, gp83, gp52, and gp45. The anti-gA monoclonal antibody neutralized VZV infectivity in the absence of complement. All eight anti-gC monoclonal antibodies neutralized only in the presence of complement. An anti-gB monoclonal antibody obtained from another laboratory also neutralizes in the absence of complement. Since the above reactivities account for all major detectable VZV glycoprotein species, the data strongly suggest that VZV has three major glycoprotein genes which encode glycosylated polypeptides with neutralization epitopes.

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mentioning

confidence: 99%

Three major glycoprotein genes of varicella-zoster virus whose products have neutralization epitopes

Keller¹,

Neff²,

Ellis³

1984

J Virol

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“…These slides were stored at -70°C for future use. To monitor the degree of infection and ensure the optimal number of infectious foci (usually 18 to 20 foci per chamber), we tested each slide preparation by immunofluorescent-antibody (IFA) staining with specific antisera to the particular virus (10,(12)(13)(14)(15).…”

Section: Methodsmentioning

confidence: 99%

Comparison of biotinylated DNA and RNA probes for rapid detection of varicella-zoster virus genome by in situ hybridization

Forghani

Hurst

1991

J Clin Microbiol

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We describe a general method for the production of nonisotopic DNA and RNA probes for the detection of the varicella-zoster virus (VZV) genome by in situ hybridization. VZV DNA was extracted from purified viral nucleocapsids, cleaved with restriction enzyme (RE) BamHI, and cloned into plasmid pBR322 by the standard vector insert procedure. We cloned over 85% of the VZV genome and obtained 18 recombinants. Plasmids containing the B, F, G, H, and J fragments of VZV DNA were labeled by the nick translation method with biotin-11-dUTP as the dTTP analog. Additionally, the B fragment was cleaved with RE AvaI, subcloned into the plasmid pGEM-4 transcription vector, and subsequently linearized with REs PstI and EcoRI. RNA was transcribed with T7 or SP6 polymerase, with a substitution of allylamine-UTP as the UTP analog, and labeled with r-caproylamidobiotin-N-hydroxysuccinimide ester. The DNA and RNA probes were used under fullstringency conditions for in situ hybridization with alkaline phosphatase as the detector and 5-bromo-4-chloro-3-indolyl phosphate-Nitro Blue Tetrazolium as the substrate. When tested under comparable conditions, the RNA probe was slightly more sensitive than was the DNA probe; both probes showed homology only with VZV-infected cells and clinical tissues and not with the other herpesviruses. Probes prepared from variable regions of the genome (fragments F and J) performed as well as did those from conserved regions (fragments B, G, and H). Biotinylated probes have distinct advantages over isotopic probes and retain their full potency for more than 2 years when stored properly.

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“…Immune reagents. The production and serological reactivities of VZV monoclonal antibodies have been described previously (9). The pool of monoclonal antibodies used as detector antibodies in the capture assays consisted of five antibodies (mouse ascitic fluids) with specificities for different glycoproteins of VZV (B. Forghani, K. W. Dupuis, and N. J. Schmidt, submitted for publication).…”

Section: Methodsmentioning

confidence: 99%

Antibody class capture assays for varicella-zoster virus

Forghani¹,

Myoraku²,

Dupuis³

et al. 1984

J Clin Microbiol

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Pooled monoclonal antibodies to varicella-zoster virus (VZV) were used as "detector" antibodies in a four-phase enzyme immunofluorescence assay for determination of immunoglobulin M (IgM), IgA, and IgG antibodies to VZV. Polyclonal antisera specific for heavy chains of human IgM, IgA, and IgG were employed as "capture" antibodies on the solid phase. The antibody class capture assay (ACCA) for VZV IgM antibody detected high titers of virus-specific IgM in all patients with varicella and in 5 of 10 zoster patients. VZV IgM antibody was not detected in patients with primary herpes simplex virus infections or in other individuals without active VZV infection, with one exception, a patient with encephalitis who had other serological findings compatible with a reactivated VZV infection. VZV-specific IgA and IgG antibody titers demonstrable by ACCA were compared with those measured by solid-phase indirect enzyme immunofluorescence assay (EIFA). VZV IgA antibody titers detected in patients with varicella and zoster were variable and could not be considered to be reliable markers of active VZV infection. IgA antibody titers detected by ACCA tended to be higher than those demonstrated by solid-phase indirect EIFA in varicella and zoster patients. VZV IgG antibody titers detected by ACCA in patients with varicella, and to a lesser extent in zoster patients, were as high as or higher than those demonstrated by solid-phase indirect EIFA. However, ACCA was totally insensitive in detecting VZV IgG antibody in individuals with past infections with VZV and would not be a suitable approach for determination of immunity status to VZV.

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Serological reactivity of some monoclonal antibodies to varicella-zoster virus

Cited by 22 publications

References 11 publications

Three major glycoprotein genes of varicella-zoster virus whose products have neutralization epitopes

Three major glycoprotein genes of varicella-zoster virus whose products have neutralization epitopes

Comparison of biotinylated DNA and RNA probes for rapid detection of varicella-zoster virus genome by in situ hybridization

Antibody class capture assays for varicella-zoster virus

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