Serological profiling of rabies antibodies by enzyme-linked immunosorbent assay and its comparative analysis with rapid fluorescent focus inhibition test in mouse model

Abstract

Aim: In this study, we have used enzyme-linked immunosorbent assay (ELISA) as an alternative test to replace the cumbersome rapid fluorescent focus inhibition test (RFFIT) to ascertain the immune status of immunized mice against rabies virus. Materials and Methods: Rabies is a devastating disease worldwide caused by rabies virus. Proper usage of pre- or post-exposure rabies vaccine can prevent the disease transmission. In this study, mice were immunized with Vero cell-adapted inactivated rabies vaccine. RFFIT was used as a test to determine the serum neutralizing titers in infected/vaccinated mice. Seroprofiling of mice sera was done in vitro by ELISA. Results: Twenty-one days post-immunization, both ELISA and RFFIT assays indicated similar antibody levels in mice sera that were immunized with Vero cell-adapted inactivated rabies vaccine. Both the tests were correlated, and the linearity was verified by the regression line (R2=0.979). Conclusion: In this study, we profiled the serological status of Vero cell-adapted inactivated rabies vaccine through ELISA in mice model that correlated well with the OIE gold standard test RFFIT.

A Comparative Evaluation of the Estimation of Rabies Virus Antibodies among Free-Roaming, Vaccinated Dogs in Bengaluru, India

“…Diagnostic parameters and statistical analyses showed a high agreement between the results of iELISA and RFFIT. A similar observation has been made by other researchers for ELISA with post-vaccinal human sera [ 27 ] and random samples from different breeds in India [ 28 ], as well as for human and murine sera [ 29 , 30 , 31 ]. No significant difference was also reported while investigating two commercially available anti-rabies vaccines in a five-dose vaccination regimen in apparently healthy street dogs [ 32 ].…”

Evaluation of In-House ELISA for Antirabies Antibodies Detection in Domestic Canine

“…Even though the RFFIT is known to be the most reliable test for the evaluation of successful vaccination, unfortunately, this is time-consuming, expensive, and requires a live rabies virus; sometimes, the results cannot be read of due to the cytotoxic efect on the cells [10,11]. Virus neutralization technically requires highly skilled technicians, hard to standardize, sufers from some interlaboratory variations, difcult to perform at weekly intervals, and requires containment facilities, which is only conveniently carried out in reference laboratories [10,[12][13][14]. In order to overcome this, ELISA deserves to be developed as an alternative to RFFIT [11].…”

Is the Rattus norvegicus Population Playing a Role in the Transmission of Zoonotic Diseases to Children? A Pilot Study in Tehran, Iran

“…Based on several studies conducted in different countries, R. norvegicus represent a reservoir for Leptospira spp. Moreover, it is predicted that these rodents carry other microorganisms such as Vibrio vulnificus (V. vulnificus) and Rabies virus (3,8,9). Despite the existence of an effective vaccine regimen, the rabies virus continues to be a global health concern with an estimated human death rate Copyright © 2021, Author(s).…”