Serological markers during dengue 3 primary and secondary infections

Vázquez, Susana; Pérez, Ana B.; Ruiz, Didye; Rodríguez, R; Pupo, Maritza; Calzada, Naifi; González, Lester; González, Daniel; Castro, Osvaldo; Serrano, Teresita; Guzmán, María G.

doi:10.1016/j.jcv.2004.10.013

Cited by 39 publications

(48 citation statements)

References 30 publications

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“…Statistical significance was P 0.05. infection may be related to inefficient detection of NS1 during secondary infections. [35][36][37][38] In summary, these results suggest that during primary infections, levels of circulating NS1 are higher in patients with DHF than in patients with DF. Nonetheless, our results and other results 18,20 also suggest that concentrations of circulating NS1 are affected by the infecting serotype and the immunologic status (i.e., primary or secondary infection) of the patient.…”

Section: Discussionmentioning

confidence: 70%

Determination of Viremia and Concentration of Circulating Nonstructural Protein 1 in Patients Infected with Dengue Virus in Mexico

Cruz-Hernández¹,

Flores-Aguilar²,

González-Mateos³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Higher levels of viremia and circulating nonstructural protein 1 (NS1) have been associated with dengue disease severity. In this study, viremia and circulating NS1 levels were determined in 225 serum samples collected from patients in Mexico infected with dengue virus serotypes 1 and 2 (DENV-1 and DENV-2). Patients with dengue hemorrhagic fever (DHF) who were infected with DENV-1 showed higher levels of circulating NS1 than patients with dengue fever (DF) (P = 0.0175). Moreover, NS1 levels were higher in patients with primary infections with DENV-1 than in patient infected with DENV-2 (P 0.0001) and in patients with primary infections with DENV-2 than in patients with secondary infections with DENV-2 (P = 0.0051). Unexpectedly, viremia levels were higher in patients with DF than in those with DHF infected with either DENV-1 or DENV-2 (P = 0.0019 and P = 0.001, respectively) and in patients with primary infections than those with secondary DENV-2 infections (P 0.0001). Results indicate that levels of circulating NS1 vary according to the infecting serotype, immunologic status (primary or secondary infection), and dengue disease severity.

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Section: Discussionmentioning

confidence: 70%

Determination of Viremia and Concentration of Circulating Nonstructural Protein 1 in Patients Infected with Dengue Virus in Mexico

Cruz-Hernández¹,

Flores-Aguilar²,

González-Mateos³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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“…3,4 The impact of dengue on the natural course of pregnancy and labour and the maternal and fetal outcomes were noted.…”

Section: Methodsmentioning

confidence: 99%

A study of dengue fever in pregnancy and its maternal and fetal prognosis

Gehlot

Yadav

Sharma

et al. 2017

Int J Reprod Contracept Obstet Gynecol

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Background: Dengue is a vector borne viral disease. Female Anopheles mosquito is the vector for the disease. Recently, there is an increase in the incidence of dengue fever in adult population in South Asian countries. With increasing rate of adult dengue fever victims, the number of infected pregnant women has also been increased. Dengue, during pregnancy may be associated with various complications, including abortions, preterm delivery, maternal mortality, low birth weight, neonatal admissions and fetal anomalies. Timely intervention can improve the maternal as well as fetal outcome. This study was aimed to assess the clinical profile, maternal and fetal outcome of dengue fever during pregnancy.Methods: The study was carried out on 25 pregnant females diagnosed and serologically confirmed to have dengue fever and were admitted in Umaid hospital, associated to Dr. S. N. Medical College Jodhpur, Rajasthan, India. Patients were included irrespective of the period of gestation of contracting the disease. Serological testing for dengue virus specific antigen and antibody was done for the diagnosis of dengue fever. The World Health Organization (WHO) classification and case definitions 2009 were used to categorize the dengue patients. A predesigned proforma was used to collect data related to maternal and fetal consequences both during pregnancy and at birth, as well as the effect on the newborn. Informed and written consent was taken from all those who participated in the study.Results: Thrombocytopenia (platelet count <1.5lakh/mm3) was found in 22 (88%) patients out of which 6 (24%) of them had platelet count below 20,000 cells/mm3 and 3(12%) patients required platelet transfusion. Other complications observed were spontaneous abortions (4%); preterm birth (16%), oligohydramnios (8%) and antepartum hemorrhage (4%). One patient was admitted to Intensive Care Unit. Fetal distress and meconium stained amniotic fluid was observed in 16% and 12% patients respectively. Adverse fetal outcome was observed in form of low birth weight, prematurity. 8% of the babies required NICU admission and 4% were Intra Uterine Fetal Death (IUFD).Conclusions: Maternal infection with the dengue virus during antenatal period represents a real risk of premature birth. Early onset or late onset in pregnancy appeared to have a bad prognosis. A high index of clinical suspicion is essential in any pregnant woman with fever during the epidemic. The treatment of dengue in pregnancy is mainly conservative as in non-pregnant adults. In case of high risk cases early referral to well-equipped health centres where technical, transfusion and intensive care facilities are available may prove lifesaving.

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“…Some authors have described IgE antibodies elevation in patients with dengue infections. [46] found both total and specific increased of IgE levels in patients with DHF, [47] observed higher levels of total IgE in dengue patients contrary to control group (subjects with no exposure); [48] observed higher levels of specific IgE in DHF/DSS cases than DF cases and healthy individuals; [13] found significant differences of IgE OD ratios between primary and secondary dengue cases, observing the higher values for the last group. Similar to IgA response, IgE OD ratios were detectable in secondary D and SD infection while in primary was absent.…”

Section: Discussionmentioning

confidence: 99%

“…In-house ELISA tests were used with the conditions previously established: IgM, IgA and IgE antibody capture immunoassays (MAC-ELISA, AAC-ELISA, EAC-ELISA) and ELISA Inhibition Method (EIM) for IgG antibody detection [13] [14]. A serum sample was considered positive by MAC and AAC-ELISA when the optical density ratio (OD ratio) was ≥2 and for EAC-ELISA test ≥ 1.4.…”

Section: Elisa Testsmentioning

confidence: 99%

Virological and Serological Markers in Dengue Patients from Venezuela and Nicaragua

Ruiz¹,

Vázquez²,

Villegas³

et al. 2017

OALib

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Latest estimates suggest 390 million infections of dengue occur each year, of which 100 million results in symptomatic disease. The new clinical classification proposed by WHO-TRD 2009 simplifies dengue fever (DF) into dengue (D) and severe dengue (SD) with an intermediary group of "dengue with warning signs" (DWS). Early, sensitive and specific diagnosis of dengue is needed for appropriate patient management as well as for early epidemic detection. Virological and serological markers in sera from dengue confirmed patients from Venezuela and Nicaragua were evaluated. A total of 232 sera collected in the acute (days 1 to 5 of fever onset) and convalescent (days 7 to 14) phases of illness were tested by Platelia NS1 antigen ELISA and IgM, IgA and IgE capture ELISA methods. NS1 was detected with highest OD values in the acute phase for primary and secondary cases. IgM was detected in primary and secondary groups but the highest OD figures were observed after defervescence day. IgA and IgE detection were almost absent for primary cases, contrary to secondary cases which showed higher values but after defervescence day. NS1 could be used as effective diagnostic marker in the acute phase of illness for primary and secondary dengue infections. IgM detection is still an invaluable tool for routine dengue diagnosis but delay. IgA detection could define secondary infection in early phase of illness. IgA/IgE could be evaluated as prognostic markers associated to SD cases.

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Serological markers during dengue 3 primary and secondary infections

Cited by 39 publications

References 30 publications

Determination of Viremia and Concentration of Circulating Nonstructural Protein 1 in Patients Infected with Dengue Virus in Mexico

Determination of Viremia and Concentration of Circulating Nonstructural Protein 1 in Patients Infected with Dengue Virus in Mexico

A study of dengue fever in pregnancy and its maternal and fetal prognosis

Virological and Serological Markers in Dengue Patients from Venezuela and Nicaragua

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