Serological investigation of Mycoplasma genitalium in infertile women

Clausen, Helle Friis; Fedder, Jens; Drasbek, Mette; Nielsen, Peter Kresten; Toft, Bente Skovsby; Ingerslev, Hans Jakob; Birkelund, Svend; Christiansen, Gunna

doi:10.1093/humrep/16.9.1866

Cited by 175 publications

(168 citation statements)

References 19 publications

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“…M. pneumoniae strain FH and M. genitalium G37 (ATCC) were cultured in 10 ml SP4 medium (Tully et al, 1979) in TPP tissue-culture flasks and incubated at 37 uC. After growth for 48 h, the medium changed colour from red to orange, indicating the exponential growth phase (Clausen et al, 2001). The mycoplasmas were scraped into 10 ml growth medium containing penicillin (100 U ml 21 ).…”

Section: Methodsmentioning

confidence: 99%

Interaction between the P1 protein of Mycoplasma pneumoniae and receptors on HEp-2 cells

et al. 2007

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The human pathogen Mycoplasma pneumoniae can cause atypical pneumonia through adherence to epithelial cells in the respiratory tract. The major immunogenic protein, P1, participates in the attachment of the bacteria to the host cells. To investigate the adhesion properties of P1, a recombinant protein (rP1-II) covering amino acids 1107-1518 of the P1 protein was produced. This protein inhibited the adhesion of M. pneumoniae to human HEp-2 cells, as visualized in a competitive-binding assay using immunofluorescence microscopy. Previous studies have shown that mAbs that recognize two epitopes in this region of P1 also reduce M. pneumoniae adhesion. Therefore, peptides covering these epitopes, of 8 and 13 aa, respectively, were synthesized to further investigate the adhesion region. None of these synthetic peptides reduced the binding of M. pneumoniae to the receptors on the host cells. Instead, 10 overlapping synthetic peptides covering the whole of rP1-II were evaluated in the competitivebinding assay using immunofluorescence microscopy. A reduction in the number of M. pneumoniae microcolonies was seen, which was confirmed for five peptides using a POLARstar OPTIMA reader to measure fluorescence intensity. The number of M. pneumoniae microcolonies adhering to the host cells was significantly reduced by these five peptides. Further investigations showed that inhibiting peptide 7 (amino acids 1347-1396) of the major adhesin protein P1 bound directly to host receptors, suggesting that the observed M. pneumoniae-inhibiting peptides occupied HEp-2 receptors, which are otherwise available for P1-mediated M. pneumoniae adhesion.

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Section: Methodsmentioning

confidence: 99%

Interaction between the P1 protein of Mycoplasma pneumoniae and receptors on HEp-2 cells

et al. 2007

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“…IgG antibodies were detected by the immunoblotting method as described [Clausen et al 2001]. Briefly, 100 mg of the whole-cell-lysate proteins of M. genitalium G37 were boiled and separated by SDS-PAGE.…”

Section: Immunoblotting For Detection Of Antibodies Against M Genitamentioning

confidence: 99%

“…Secondary antibodies used were: alkaline phosphatase conjugated goat anti-human IgG (H þ L) (Jackson Immunoresearch Laboratories, West Grove, USA) diluted 1:1,000 or goat anti-rabbit IgG (H þ L) (BioRad, Hercules, CA, USA) diluted 1:3,000. MgPa membrane protein of Mycoplasma genitalium was localized on the blot by recombinant polyclonal rabbit antibody PabMgPa [Clausen et al 2001]. The band intensities were described as weak, intermediate or strong and only patient's sera that reacted intermediately or strongly with MgPa protein were defined as seropositive.…”

Section: Immunoblotting For Detection Of Antibodies Against M Genitamentioning

confidence: 99%

“…These women can be regarded as a higher risk population since they had unprotected sex, resulting in pregnancy and the possibility of exposure to sexually transmitted bacteria. In addition, we decided to perform this study because of the results from the previous studies examining the presence of antibodies to genital mycoplasmas and the correlation with tubal factor infertility [Clausen et al 2001;Baczynska et al 2005]. The infertile patients examined in the studies must have had an infection at an earlier stage in their life.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence ofMycoplasma genitalium, Mycoplasma hominisandChlamydia trachomatisAmong Danish Patients Requesting Abortion

Baczynska

Hvid

Lamy

et al. 2008

Systems Biology in Reproductive Medicine

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Prevalence of Mycoplasma genitalium, Mycoplasma hominis and Chlamydia trachomatis Among Danish Patients Requesting AbortionThe aim of the study was to determine lower genital tract carriage rate of Mycoplasma genitalium (M. genitalium) and to compare it to the carriage rates of Mycoplasma hominis (M. hominis ) and Chlamydia trachomatis (C. trachomatis) among 102 women requesting termination of pregnancy at the Horsens Hospital in Denmark. Real-Time PCR was used for the detection of bacterial DNA, and the presence of antibodies to the three microorganisms was determined by ELISA and immunoblotting. Real-Time PCR detected M. genitalium in one swab sample (0.98%) only, while the prevalence of C. trachomatis was high (15.69%) and M. hominis colonization (18.63%) was similar to colonization observed among sexually experienced adults. There was a significant difference in prevalence of M. hominis infection in the different age groups. C. trachomatis load in the cervical samples was significantly higher among young patients. There was no correlation between the presence of genital infection with C. trachomatis and genital mycoplasmas and no correlation between the presence of antibodies to these bacteria. In conclusion, in Danish patients it is not necessary to test for M. genitalium before abortion since less than 1% were found positive. The prevalence of genital C. trachomatis infections was high among the abortionseeking patients.

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“…Universidad de Chile Walter Gübelin Harcha wgubelin@ctcinternet.cl toria pelviana (EIP) [12][13] e infertilidad 14 . Sin embargo, como ocurre con los otros micoplasma genitales, el microorganismo también ha sido detectado en personas sin signos o síntomas de infección genital 3,6 .…”

Section: Universidad De Los Andes (Wgh)unclassified

Aplicación de método molecular en la detección de Mycoplasma genitalium en hombres y en mujeres embarazadas

et al. 2006

Rev. chil. infectol.

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Serological investigation of Mycoplasma genitalium in infertile women

Abstract: This study indicates that M. genitalium may be an independent risk factor in the development of an inflammatory process leading to scarring of the uterine tubes in women and thereby causing infertility.

Cited by 175 publications

References 19 publications

Interaction between the P1 protein of Mycoplasma pneumoniae and receptors on HEp-2 cells

Interaction between the P1 protein of Mycoplasma pneumoniae and receptors on HEp-2 cells

Prevalence ofMycoplasma genitalium, Mycoplasma hominisandChlamydia trachomatisAmong Danish Patients Requesting Abortion

Aplicación de método molecular en la detección de Mycoplasma genitalium en hombres y en mujeres embarazadas

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