Serological evidence of infection with dengue and Zika viruses in horses on French Pacific Islands

Beck, Cécile; Leparc-Goffart, Isabelle; Desoutter, Denise; Debergé, Estelle; Bichet, Hervé; Lowenski, Steeve; Marine, Dumarest; Gonzalez, Gaëlle; Migné, Camille Victoire; Vanhomwegen, Jessica; Zientara, Stéphan; Durand, Benoı̂t; Lecollinet, Sylvie

doi:10.1371/journal.pntd.0007162

Cited by 23 publications

(18 citation statements)

References 47 publications

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“…When enough volume was available, bird serum samples were tested first using a commercial ID Screen West Nile Competition Multi-species ELISA kit, IDvet, Montpellier, France. Assays were performed according to the method described in Beck et al [50], and interpreted according to manufacturer's instructions. This ELISA test uses plates pre-coated with the envelope (E) protein of WNV and measures the competition between antibodies present in the animal serum tested and a monoclonal anti-WNV E antibody conjugated to horseradish peroxidase (HRP).…”

Section: Laboratory Analysesmentioning

confidence: 99%

“…Samples with % S/NC > 50% were considered negative. All positive samples by ELISA and all sera with insufficient volume and that could not be tested by ELISA were analyzed by microneutralization tests (MNT) on Vero NK cells, with the WNV lineage 1 IS-98-ST1 and USUV 206795/Italy 2012 strains (kindly provided by Philippe Desprès, IPP and D. Lelli, IZSLER respectively) following the protocol described in Beck et al [50]. Sera of birds was heat-inactivated at 56 • C for 30 min, then serially diluted (dilution factor of 2, 1/5 to 1/320) and mixed with a volume of 50 µL of Dulbecco's Modified Eagle's medium (DMEM), containing 100 tissue culture infectious dose 50 of WNV strain IS-89-ST1, or USUV strain 206795.…”

Section: Laboratory Analysesmentioning

confidence: 99%

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Serological Evidence of West Nile and Usutu Viruses Circulation in Domestic and Wild Birds in Wetlands of Mali and Madagascar in 2008

Chevalier

Marsot

Molia

et al. 2020

IJERPH

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The geographical distribution and impact on animal and human health of both West Nile and Usutu viruses, two flaviviruses of the Japanese encephalitis complex, have been increasing during the past two decades. Both viruses circulate in Europe and Africa within a natural cycle between wild birds and mosquitoes, mainly from the Culex genus. We retrospectively analyzed sera from domestic and wild birds sampled in 2008 in two wetlands, namely the Inner Niger Delta, Mali, and the Lake Alaotra area, Madagascar. Sera were first tested using a commercial ID Screen West Nile Competition Multi-species ELISA kit. Then, positive sera and sera with insufficient volume for testing with ELISA were tested with a Microneutralization Test. In Mali, the observed seroprevalence in domestic birds was 28.5% [24.5; 32.8] 95%CI, 3.1 % [1.8; 5.2] 95%CI, 6.2% [3.4; 10.2] 95%CI and 9.8 % [7.3; 12.8] 95%CI, for West Nile virus (WNV), Usutu virus (USUV), undetermined flavivirus, and WNV/USUV respectively. Regarding domestic birds of Madagascar, the observed seroprevalence was 4.4 % [2.1; 7.9]95%CI for WNV, 0.9% [0.1; 3.1] 95%CI for USUV, 1.3% [0.5; 2.8] 95%CI for undetermined flavivirus, and null for WNV/USUV. Among the 150 wild birds sampled in Madagascar, two fulvous whistling-ducks (Dendrocygna bicolor) were positive for WNV and two for an undetermined flavivirus. One white-faced whistling-duck (Dendrocygna viduata) and one Hottentot teal (Spatula hottentota) were tested positive for USUV. African and European wetlands are linked by wild bird migrations. This first detection of USUV—as well as the confirmed circulation of WNV in domestic birds of two wetlands of Mali and Madagascar—emphasizes the need to improve the surveillance, knowledge of epidemiological patterns, and phylogenetic characteristics of flavivirus in Africa, particularly in areas prone to sustained, intense flavivirus transmission such as wetlands.

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Section: Laboratory Analysesmentioning

confidence: 99%

Section: Laboratory Analysesmentioning

confidence: 99%

Serological Evidence of West Nile and Usutu Viruses Circulation in Domestic and Wild Birds in Wetlands of Mali and Madagascar in 2008

Chevalier

Marsot

Molia

et al. 2020

IJERPH

Self Cite

View full text Add to dashboard Cite

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“…Serological diagnosis is especially challenging in regions with flavivirus cocirculation. In Brazil, epidemiological studies have demonstrated the presence of other flaviviruses of public health importance in the equine population, such as dengue virus (DENV), Zika virus (ZIKV), Mayaro virus (MAYV), Saint Louis Encephalitis Virus (SLEV), Ilhéus virus (ILHV), Iguape virus (IGUV), Rocio virus (ROCV) and Cacipacore virus (CACV) (Castro-Jorge et al, 2019;Beck et al, 2019). Since horses can be infected and seroconvert against these arboviruses of human importance, they serve as sentinels for these viruses as well.…”

Section: Important Topics To Consider In Brazilmentioning

confidence: 99%

Epidemiological surveillance of West Nile virus in the world and Brazil

Costa¹,

Bayeux²,

Silva

et al. 2020

Braz. J. Vet. Res. Anim. Sci.

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West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus that is maintained in nature by a zoonotic transmissioncycle between avian hosts and ornithophilic mosquito vectors, mostly from the Culex genus. Until the 1990s, WNV wasconsidered to be an old-world arbovirus, but in 1999, WNV emerged in the United States (US) and spread rapidly, becoming amajor threat to public health. WNV adapted to the transmission cycle involving American mosquitoes and birds and reachedCentral and South America in subsequent years. In 2003, the National West Nile Fever Surveillance System was created in Brazilbased on serological screening of animals and sentinel vectors, as recommended by the Pan American Health Organization(PAHO) and the World Health Organization (WHO). Since 2008, serological evidence of WNV infection in Brazilian horseshas been reported, and the circulation of WNV has been monitored through the regular serological screening of sentinel horsesand reporting of encephalomyelitis cases. Horses are highly susceptible to WNV infection, and outbreaks of neurologicaldisease among horses often precede human cases. In this regard, equine surveillance has been essential in providing earlywarning to public and animal health authorities in several countries, including Brazil. This demonstrates the need for animaland public health intervention programs to allocate resources to make veterinarians aware of the role they can play in thehuman surveillance processes by monitoring horses. This review discusses the importance of equine surveillance and the gapthat veterinarians can fill on the front line in human surveillance, in Brazil and worldwide, in the context of “One Health”

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“…However, the CDC does not specify which DENV serotype or whether multiple serotypes should be used [47]. A cursory examination into recent ZIKV serological studies shows that many laboratories employ a single strain of DENV for neutralization assays [48][49][50]. This could also lead to misdiagnosis of ZIKV since data throughout this manuscript have shown that neutralization of DENV by mAbs and subject sera is serotype specific.…”

Section: Discussionmentioning

confidence: 99%

Strain-Dependent Activity of Zika Virus and Exposure History in Serological Diagnostics

et al. 2020

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Zika virus (ZIKV) circulates as two separate lineages, with significant genetic variability between strains. Strain-dependent activity has been reported for dengue virus, herpes simplex virus and influenza. Strain-dependent activity of subject specimens to a virus could be an impediment to serological diagnosis and vaccine development. In order to determine whether ZIKV exhibits strain-dependent activity when exposed to antibodies, we measured the neutralizing properties of polyclonal serum and three monoclonal antibodies (ZKA185, 753(3)C10, and 4G2) against three strains of ZIKV (MR−766, PRVABC59, and R103454). Here, MR−766 was inhibited almost 60% less by ZKA185 than PRVABC59 and R103454 (p = 0.008). ZKA185 enhanced dengue 4 infection up to 50% (p = 0.0058). PRVABC59 was not inhibited by mAb 753(3)C10 while MR−766 and R103453 were inhibited up to 90% (p = 0.04 and 0.036, respectively). Patient serum, regardless of exposure history, neutralized MR−766~30%−40% better than PRVABC56 or R103454 (p = 0.005−0.00007). The most troubling finding was the significant neutralization of MR−766 by patients with no ZIKV exposure. We also evaluated ZIKV antibody cross reactivity with various flaviviruses and found that more patients developed cross-reactive antibodies to Japanese encephalitis virus than the dengue viruses. The data here show that serological diagnosis of ZIKV is complicated and that qualitative neutralization assays cannot discriminate between flaviviruses.Trop. Med. Infect. Dis. 2020, 5, 38 2 of 14 with ZIKV, dengue virus (DENV) and other endemic flaviviruses is recommended by the Centers for Disease Control and Prevention ( CDC) to confirm diagnosis when RT-PCR is negative, and IgM ELISA is "not negative" [7]. Unfortunately, even PRNT can be problematic for diagnosis with over half of patients exhibiting significant neutralization of ZIKV, DENV, and other flaviviruses when positive for ZIKV IgM [9].ZIKV co-circulates with other flaviviruses, including DENV, West Nile virus (WNV), Japanese Encephalitis virus (JEV) and Yellow Fever virus (YFV). Antibody-based assays can be problematic as serological cross-reactivity can confound results [10][11][12]. Furthermore, previous exposures and co-infections can further complicate diagnostic tests [13][14][15]. Additional difficulties arise when diagnosing clinical samples in different locations as reference virus and antigen sources can produce divergent results in response to locally circulating viral isolates [16].Prior to the 2016 outbreak, there were very few ZIKV isolates available for use, which included the prototype MR−766 isolate as well as IbH 30656 a Nigerian isolate and DAK AR 41524 from Senegal (BEIResources.org). MR−766 had been extensively characterized prior to the outbreak and was the reference strain used by many researchers in the early days following emergence [6,[17][18][19][20]. Within a year of emergence, the CDC prototype PRVABC59 was released for use through BEI resources and many labs began using this modern isolate for their research and dia...

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Serological evidence of infection with dengue and Zika viruses in horses on French Pacific Islands

Cited by 23 publications

References 47 publications

Serological Evidence of West Nile and Usutu Viruses Circulation in Domestic and Wild Birds in Wetlands of Mali and Madagascar in 2008

Serological Evidence of West Nile and Usutu Viruses Circulation in Domestic and Wild Birds in Wetlands of Mali and Madagascar in 2008

Epidemiological surveillance of West Nile virus in the world and Brazil

Strain-Dependent Activity of Zika Virus and Exposure History in Serological Diagnostics

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