Serological evidence of hantavirus infection in rural and urban regions in the state of Amazonas, Brazil

Gimaque, João Bosco Lima; Bastos, Michele de Souza; Braga, Wornei Silva Miranda; Oliveira, Cíntia Mara Costa de; Castilho, Márcia da Costa; Figueiredo, Regina Maria Pinto de; Galusso, Elizabeth dos Santos; Itapirema, Evaulino; Figueiredo, Luíz Tadeu Moraes; Mourão, Maria Paula Gomes

doi:10.1590/s0074-02762012000100019

Cited by 21 publications

(21 citation statements)

References 12 publications

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“…Our results showed a higher rate of seropositivity to hantavirus (13.6%) when compared with other serological studies conducted in all regions of Brazil by using the same methodology (0.6% to 4.7%) ( 5) (6) (7) (8) (9) (10) . In this study, we used ELISAs that were based on the recombinant N protein of ARAV, despite not having this virus described in MT yet.…”

supporting

confidence: 43%

Serological evidence of hantavirus infection in an urban area in Mato Grosso State, Brazil

Vieira

Silva

Barreto

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction:In Brazil, Mato Grosso (MT) has the highest number of hantavirus cardiopulmonary syndrome cases. Our study aimed to identify anti-hantavirus antibodies in the sera of patients from Sinop, MT, presenting with acute febrile illness. Methods: A retrospective analysis of data for 198 sera samples assessed using enzyme-linked immunosorbent assay (ELISA) was conducted. Results: Immunoglobulins G (IgGs) against the hantavirus nucleoprotein were found in 13.6% of the tested sera. No sample had immunoglobulin M (IgM) antibodies to hantavirus. Seropositivity occurred mainly in female residents in urban areas who worked around the household. Conclusions: Our findings suggest circulation of hantavirus in Sinop.Keywords: Zoonosis. Serosurvey study. Hantavirus.Hantaviruses (genus Hantavirus, family Bunyaviridae) are emerging zoonotic viruses of great importance for public health. In South America, the rodents of the Sigmodontinae subfamily are reservoirs of these viruses. Some hantaviruses infect humans, causing either hemorrhagic fever with renal syndrome (HFRS) and/or hantavirus cardiopulmonary syndrome (HCPS) (1) .The first cases of HCPS diagnosed in Brazil occurred in 1993. Since then, more than 1,920 cases of hantavirus have been notified in all Brazilian regions, with a case fatality rate of up to 39.5%. Mato Grosso (MT) is the leading state for HCPS cases and accounts for 16.1% of the total notified cases in Brazil. In a previous study conducted in the municipality of Sinop, Mato Grosso State, we carried out an epidemiological surveillance for arboviruses in 198 sera samples from patients presenting with acute febrile illness and clinical-epidemiological diagnoses of dengue. The results showed 46 samples positive for arboviruses. However, it was intriguing that more than 70% of the patients did not have dengue or other arbovirus infections that were tested for (2) . To address the possibility of hantavirus infection, we investigated the presence of antibodies against the hantavirus nucleoprotein in these febrile patients.Sinop is a municipality located 503km from the capital Cuiabá, in a geographical transition zone between a savannah and rainforest. The city was founded in 1974 and currently has an estimated population of approximately 130,000 inhabitants with 17.1% of them living in rural areas. The economic activities include an intensive agriculture based on the grain production, animal husbandry, logging, and human services. All of the sera analyzed in this study were part of the serum bank of the Laboratory of Immunology and Molecular Biology of Universidade Federal de Mato Grosso (UFMT), Campus of Sinop, which have been previously described in detail (2) . At the time of blood collection, the patients filled out a questionnaire that contained their epidemiological data, sex, age, occupation, address and region of birth, as well as information about any contact with rural or forested areas, and contact with rodents. These data were collected to analyze the risk factors associated with infect...

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supporting

confidence: 43%

Serological evidence of hantavirus infection in an urban area in Mato Grosso State, Brazil

Vieira

Silva

Barreto

et al. 2016

Rev. Soc. Bras. Med. Trop.

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“…The 0.6% seroprevalence of antibodies against hantavirus detected by us is similar to the serological survey conducted by Gimaque et al (2012) that used the same technique and found an IgG seropositivity of 0.6% in samples from the urban and rural areas of four municipalities in the State of Amazon. Another study, conducted in cities in the State of Santa Catarina near the border between Brazil and Argentina (Souza et al, 2011), found a much higher seropositivity (3.5%) for IgG antibodies to hantavirus compared with our study; however, most of the HCPS have occurred in this country's southern and southeast regions.…”

Section: Discussionsupporting

confidence: 87%

A Seroepidemiological Survey of Hantavirus in Ilheus County

Moreli

Costa

Pariz

2012

American Journal of Virology

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Hantaviral diseases are infections caused by a group of viruses that belong to the genus hantavirus and the family Bunyaviridae. In Brazil, several cases of hantavirus have been detected with the use of serological methods including immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA). To date, 1.573 cases have been reported in several states across the country with a fatality rate of 41%. No seroepidemiologic study has ever been conducted in the city of, or region surrounding, Ilheus, where large remnants of the Atlantic Forest provide shelter for a variety of rodents. The authors used the N recombinant protein of Araraquara virus as antigen to test for the presence of hantavirus-specific IgG antibodies by ELISA in samples provided by blood donors in the city of Ilheus, State of Bahia. The authors collected blood samples from 500 donors. Among these, 93.6% of the donors came from an urban area and 6.4% were from a rural area. A total of 0.6% of the donors was seropositive for anti-hantavirus IgG antibodies. This positivity was detected only in males at a rate of 1.1%. However, there was no observed association for hantavirus seropositivity between the genders (p = 0.252). There was a higher frequency of seropositive individuals aged between 31 and 40 years (1.6%), although the difference observed was not statistically significant (p = 0.41). There was also a higher rate of seropositive individuals among donors from rural areas (6.6%); the association for seropositivity between participants in rural and urban areas was statistically significant (p = 0.011) by Fisher's exact test (p<0.05). The present study describes Araraquara virus circulation in this region for the first time. The results of this study provide the basis for future investigation of the hantaviruses that are associated with cardiopulmonary syndrome and the other antigenic ally related hantaviruses in this region.

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“…Diante das evidências identificadas no presente estudo que comprovam a circulação de hantavírus em roedores, no Estado do Acre, e em humanos, apontadas na literatura 18,20 , sugere-se que existam subnotificações de casos humanos por meio dos serviços de saúde e da Quanto aos ambientes em que os roedores silvestres sororreagentes foram capturados, estes possuíam características de área de plantação de cana-de-açúcar, de pastagem para bovinocultura com capim-napier e peridomiciliar, caracterizada por ser área limítrofe entre capim-braquiária e cultivo de cana-de-açúcar.…”

Section: Materiais E Métodosunclassified