Serological evidence of canine exposure to arthropod-borne pathogens in different landscapes in Rio de Janeiro, Brazil

Willi, Liliane Maria Valentim; Mendes-de-Almeida, Flavya; Souza, Celeste da Silva Freitas de; Laeta, Tainá; Paiva, Jonimar Pereira; Miranda, Marcia Gonçalves Nobre de; Knackfuss, Fabiana Batalha; Labarthe, Norma

doi:10.1016/j.vprsr.2016.11.003

Cited by 4 publications

(3 citation statements)

References 23 publications

(24 reference statements)

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“…Therefore, the possibility of interference from other infectious agents in these areas, including Trypanosoma cruzi, Trypanosoma caninum, Toxoplasma gondii, Neospora caninum, Babesia canis, and Ehrlichia canis, as previously observed 11,17,18 , must be considered. This possibility is reinforced by the reported 47% canine seroprevalence of parasites transmitted by ticks in the eastern region of Rio de Janeiro 19 and 80% seroprevalence in the surrounding area of the natural reserve in the state of Mato Grosso (unpublished data).…”

Section: Discussionmentioning

confidence: 94%

Evaluation of DPP® and SNAP® Rapid Tests for diagnosis of Leishmania infantum canine infections

Souza

Silva

Labarthe

2019

Rev. Soc. Bras. Med. Trop.

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Introduction: Visceral leishmaniasis is a disease that affects humans, wildlife, and domestic species. Since dogs play a key role in urban Leishmania spp. transmission, the Brazilian government maintains the Monitoring and Control Program of Visceral Leishmaniasis (VLMCP) in endemic regions, which promotes awareness campaigns aiming to enhance the control of the infection. The VLMCP recommends the Dual Path Platform (DPP ® ) canine visceral leishmaniasis test (Bio-Manguinhos, Brazil) for screening and enzyme-linked immunosorbent assay to confirm the infection. The DPP ® test is produced and distributed by the Health Ministry to the Municipal Health Centers responsible for the local VLMCP. The test is not available to all the clinics, forcing some veterinarians to use other rapid tests for screening and diagnosis of this disease in their daily routine. Methods: The present study was conducted to compare the performance of the DPP ® and SNAP ® tests using sera from the dogs with confirmed infections of L. infantum and from the dogs with no previous testing, residing in areas with a low Leishmania infection. Results: There was 97.0% agreement between the two tests. Sensitivity and specificity of the SNAP ® test were 96.3% and 100%, respectively. Agreement between both the antibody tests and the parasitological detection methods was 96.8%. The DPP ® test had 95.8% sensitivity and 100% specificity. Conclusions: The SNAP ® and the DPP ® tests were virtually equivalent in terms of detection of canine antibodies against L. infantum, and both the tests demonstrated high and similar levels of sensitivity and specificity.

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Section: Discussionmentioning

confidence: 94%

Evaluation of DPP® and SNAP® Rapid Tests for diagnosis of Leishmania infantum canine infections

Souza

Silva

Labarthe

2019

Rev. Soc. Bras. Med. Trop.

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“…The study was conducted in two coastal areas of the state of Rio de Janeiro in sandbanks of the municipalities of Arraial do Cabo and Araruama, where the dogs are exposed to a high challenge, 80.8 and 58.4% respectively [ 10 ]. Both areas are located at sea level, apart from one another for 10 km.…”

Section: Main Textmentioning

confidence: 99%

Can P-glycoprotein and β-tubulin polymorphisms be used as genetic markers of resistance in Dirofilaria immitis from Rio de Janeiro, Brazil?

et al. 2018

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ObjectiveDirofilaria immitis, the causative agent of canine heartworm infection, is worldwide the most important filarid to affect domestic dogs. Prevention of this infection is done by macrocyclic lactones, but some reports on the lack of efficacy have been published. Although the actual cause of resistance is unknown, single nucleotide polymorphisms (SNPs) on a P-glycoprotein ABC transporter and β-tubulin genes have been pointed out as candidates for genetic markers of resistance. We conducted a survey to verify the presence of these suggested genetic markers in microfilariae from 30 naturally infected dogs under macrocyclic lactones treatment living in an endemic area in the state of Rio de Janeiro.ResultsThe analysis of these specific SNPs demonstrated no sign of polymorphism on the P-glycoprotein loci, while 72 and 48% of the samples were polymorphic to the first and second SNPs on β-tubulin loci, respectively. This work demonstrates that the P-glycoprotein position 11 and 618 were not polymorphic and, therefore, not suitable as a genetic marker of resistance in Rio de Janeiro whereas both β-tubulin loci were polimorphic. This work points out the difficulty of finding a universal genetic marker for resistance.Electronic supplementary materialThe online version of this article (10.1186/s13104-018-3259-z) contains supplementary material, which is available to authorized users.

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“…In Brazil, as in other South American countries, D. immitis and E. canis are the most prevalent arthropod-borne parasites (Labarthe et al, 2018;Bendas et al, 2017;Willi, et al, 2017;Labarthe et al, 2014;Vezzani et al, 2011;Guilarte et al, 2011;Melo et al, 2011;Aguiar at al., 2007;Vieira et al, 2011). This wide distribution of E. canis is probably due to the fact that its vector is the wide-spread Rhipicephalus sanguineus (Miranda & Máttar, 2015;Vieira et al, 2011;Dantas-Torres, 2008), a prevalent species all over Brazil (Labruna & Pereira 2001).…”

Section: Introductionmentioning

confidence: 99%

Serological evidence of canine arthropod-borne infections in an ecotone area of a natural reserve at the Pantanal, Brazil

et al. 2019

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Arthropod-borne infections are dependent on environmental conditions; therefore, anthropomorphic meddling may disrupt the natural balance that maintains wildlife. It is common to find dogs roaming in Brazilian natural reserves, what favors the spillover of pathogens among species. The aim of this study was to determine the canine seroprevalence of Ehrlichia canis, E. ewingii, Anaplasma platys, A. phagocytophilum, Borrelia burgdorferi, Leishmania infantum and Dirofilaria immitis using 84 serum samples from dogs from the border area of the SESC-Pantanal reserve (RPPN SESC-Pantanal 16°40´51´´S;56º17´45´´W) stored at the Laboratório de Protozoologia e Imunomodulação, Instituto Oswaldo Cruz. Samples were tested with SNAP  Canine Leishmania Antibody Test (IDEXX Laboratories) or DPP  canine visceral leishmaniasis test (Bio-Manguinhos) for the presence of L. infantum antibodies and with SNAP  4Dx Plus Test (IDEXX Laboratories) for D. immitis, Ehrlichia spp., Anaplasma spp. and B. burgdorferi seroprevalences. The seroprevalence for tick-borne parasites was 79.8%, 13.1% for L. infantum and 7.1% for D. immitis. Since tick-borne parasites were the most frequent among the examined dogs it may be suggested that these parasites, ticks and hosts display special resilience skills to overcome the hostile local conditions. The low L. infantum prevalence suggests that the local biodiversity, especially the bird abundance, depurates Leishmania circulation. The mosquito-borne D. immitis prevalence was higher than expected, suggesting that the local flooding regime provides suitable mosquito-breeding spots. On the other hand, the only known vector in the area feeds on birds, therefore reducing mosquitoes worm burden and imparing D. immitis transmission.

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Serological evidence of canine exposure to arthropod-borne pathogens in different landscapes in Rio de Janeiro, Brazil

Cited by 4 publications

References 23 publications

Evaluation of DPP® and SNAP® Rapid Tests for diagnosis of Leishmania infantum canine infections

Evaluation of DPP® and SNAP® Rapid Tests for diagnosis of Leishmania infantum canine infections

Can P-glycoprotein and β-tubulin polymorphisms be used as genetic markers of resistance in Dirofilaria immitis from Rio de Janeiro, Brazil?

Serological evidence of canine arthropod-borne infections in an ecotone area of a natural reserve at the Pantanal, Brazil

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