Serological evidence for antigenic relationships between Ehrlichia canis and Cowdria ruminantium

Kelly, Patrick J.; Matthewman, Linda; Mahan, Suman M.; Semu, S. M.; Peter, Trevor; Mason, Peter R.; Brouqui, P.; Raoult, Didier

doi:10.1016/0034-5288(94)90100-7

Cited by 36 publications

(23 citation statements)

References 12 publications

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“…For example, an Ehrlichia ruminantium -like sp. has been detected by PCR in domestic dogs in South Africa (Allsopp and Allsopp 2001) and the serologic cross-reactivity of E. canis and E. ruminantium has been demonstrated (Kelly et al 1994). Previous studies have identified Bartonella henselae in lions, but both serologic and molecular-based prevalence rates are low (Molia et al 2004;Pretorius et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and diversity of Babesia, Hepatozoon, Ehrlichia, and Bartonella in wild and domestic carnivores from Zambia, Africa

et al. 2013

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A molecular survey was conducted for several hemoparasites of domestic dogs and three species of wild carnivores from two sites in Zambia. Three Babesia spp. were detected including Babesia felis and Babesia leo in lions (Panthera leo) and a Babesia sp. (similar to Babesia lengau) in spotted hyenas (Crocuta crocuta) and a single lion. All wild dogs (Lycaon pictus) and domestic dogs were negative for Babesia . High prevalences for Hepatozoon were noted in all three wild carnivores (38-61 %) and in domestic dogs (13 %). Significantly higher prevalences were noted in hyenas and wild dogs compared with domestic dogs and lions. All carnivores were PCR negative for Ehrlichia canis , Ehrlichia ewingii, and Bartonella spp. Overall, high prevalences and diversity of Babesia and Hepatozoon were noted in wild carnivores from Zambia. This study is the first molecular characterization of Babesia from any hyena species and is the first report of a Babesia sp. closely related to B. lengau, a parasite previously only reported from cheetahs (Acinonyx jubatus ), in lions and hyenas. Although usually benign in wild carnivores, these hemoparasites can be pathogenic under certain circumstances. Importantly, data on vectors for these parasites are lacking, so studies are needed to identify vectors as well as determine transmission routes, infection dynamics, and host specificity of these hemoparasites in wildlife in Africa and also the risk of transmission between domestic animals and wildlife.

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Section: Discussionmentioning

confidence: 99%

Prevalence and diversity of Babesia, Hepatozoon, Ehrlichia, and Bartonella in wild and domestic carnivores from Zambia, Africa

et al. 2013

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“…Eight of these dogs were pCS20 probe positive, suggesting that they had acquired an E. ruminantium infection before their arrival in the country. Other workers have shown that dogs infected experimentally with E. ruminantium Crystal Springs did not become sick but showed visible pCS20 amplicons for up to 3 weeks after infection (16). This indicates that, although some dogs may show symptoms sug- gestive of ehrlichiosis, apparently healthy dogs may be asymptomatic carriers of E. ruminantium.…”

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confidence: 92%

Novel Ehrlichia Genotype Detected in Dogs in South Africa

Allsopp

2001

J Clin Microbiol

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DNA samples from dogs presenting with symptoms suggestive of canine ehrlichiosis, but with no morulae detected on blood smears, frequently failed to give a positive reaction with a North American Ehrlichia canis-specific PCR assay targeting the 16S rRNA gene. We suspected the presence of a pathogen genetically different from North American E. canis, and we performed experiments to test this hypothesis. DNA from one canine blood sample was subjected to PCR with primers designed to amplify Ehrlichia (Cowdria) ruminantium ruminantium 16S and map1 genes. Amplicon sequencing yielded 16S and map1 sequences which were more closely related to other E. ruminantium sequences than to those of any other Ehrlichia species. Fifty canine DNA samples were subjected to a PCR assay, previously found to be Cowdria-specific, which targets the pCS20 gene. Thirty-seven (74%) gave a positive signal, and 16 (32%) also gave visible amplicons after gel electrophoresis, suggesting that this E. ruminantium organism is common in the Pretoria-Johannesburg area. The organism has not been isolated in culture, so we cannot definitively state that it was responsible for the canine ehrlichiosis symptoms, although the occurrence of several similar cases suggests this to be so. Most importantly, we also do not yet know whether the organism is infective for, or causes heartwater in, ruminants.

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“…Since serological tests cannot distinguish between infection by E. canis and other Ehrlichia species (KELLY et al, 1994;ALLSOPP;ALLSOPP, 2001), the present study aimed to evaluate the presence of Ehrlichia species by molecular tools in blood samples of dogs from the municipality of Poconé, Pantanal region, Brazil, where higher canine seroprevalence of Ehrlichia spp. has been recently reported (MELO et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Ehrlichia canisin dogs from the Pantanal of Mato Grosso State, Brazil

Santos

Melo

Moraes‐Filho

et al. 2013

Rev. Bras. Parasitol. Vet.

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The present study evaluated the presence of Ehrlichia DNA in the blood samples of 320 dogs from the urban and rural areas of the municipality of Poconé, Pantanal region, Mato Grosso state, by Polymerase Chain Reaction (PCR), targeting the ehrlichial dsb gene. Risk factors for infection in dogs were also evaluated. Forty-eight (15%, 95% CI: 11.4-19.5%) dogs were positive: 25 (15.6%, 95% CI: 10.4-22.2%) from the urban area and 23 (14.4%, 95% CI: 9.3-20.8%) from the rural area (P > 0.05). Partial DNA sequence obtained from PCR products of 18 samples from the urban area and 16 samples from the rural area were 100% identical to E. canis from Brazil and the USA. This study reports the first E. canis molecular detection in dogs from the northern Pantanal region.Keywords: Dogs, tick borne disease, Ehrlichia canis, Anaplasmataceae, Poconé, Brazil. ResumoO presente estudo avaliou a presença de DNA de Ehrlichia spp. em 320 cães das áreas urbana e rural do município de Poconé, região do Pantanal de Mato Grosso, pela PCR visando o gene dsb. Os fatores de risco para a infecção em cães também foram avaliados. Quarenta e oito (15%, IC 95%: 11,4-19,5%) cães foram positivos, 25 (15,6%, IC 95%: 10,4-22,2%) da área urbana e 23 (14,37%, 95% CI: 9,3-20,8%) da área rural (P > 0,05). Sequências parciais de DNAs obtidos a partir de produtos da PCR de 18 amostras da área urbana e 16 da área rural foram 100% idênticas a E. canis do Brasil e EUA. Este estudo relata a primeira detecção molecular de E. canis em cães da região norte do Pantanal.

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Serological evidence for antigenic relationships between Ehrlichia canis and Cowdria ruminantium

Cited by 36 publications

References 12 publications

Prevalence and diversity of Babesia, Hepatozoon, Ehrlichia, and Bartonella in wild and domestic carnivores from Zambia, Africa

Prevalence and diversity of Babesia, Hepatozoon, Ehrlichia, and Bartonella in wild and domestic carnivores from Zambia, Africa

Novel Ehrlichia Genotype Detected in Dogs in South Africa

Molecular detection of Ehrlichia canisin dogs from the Pantanal of Mato Grosso State, Brazil

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