Serological evidence for antigenic differences between the Mason-Pfizer monkey virus (MPMV) and an MPMV-like virus (PMFV) detected in a malignant permanent human cell line

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The protein patterns of type D retroviruses isolated either from permanent human cells (HeLa virus, HeLa V, and HEp-2 virus, HEp-2V) or from spectacled langur (langur virus, LV), an Old World monkey, were investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Labeling with 14C-amino acids and 3H-glucosamine revealed for each of these isolates five polypeptides with molecular weights of 10,000, 12,000, 15,000, 25,000 and about 68,000 and identified the 68,000 D-protein as a glycoprotein. An additional glycoprotein with a molecular weight of 20,000 (gp20) was resolved in LV similarly as in previous studies with Mason-Pfizer monkey virus. However, in accordance with our recent analysis of a third type D isolate from human cells (PMFV), gp20 was not detectable in both HeLaV and HEp-2V regardless of the cell line in which they were grown. Thus, it appears that the type D virus isolates from human cells are slightly distinct from the presently known type D retroviruses of Old World monkeys. The relevance of this finding to the origin of the human isolates, however, remains to be shown.

Section: Comparison Of the Polypeptide Compositions Of Helav Hep-2v supporting

confidence: 76%

Section: Introductionmentioning

confidence: 98%

Type D retroviruses from human cells do not contain a minor glycoprotein shared by old world monkey type D viruses

Wunderlich¹,

Uckert²

1981

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“…If the immunosuppressive effect of retroviruses is based on true receptor-effector molecule reactions, the broad interspecies reactivity of PMFV as well as FeLV can be explained only by viral proteins which were highly conserved during evolution. In PMFV a low molecular weight suppressive component, p 10--15, has been detected (12) and some structural and immunological differences in the p 15s have been found between PMFV and MPMV (22,23,29,30). Cross-reacting antigen epitopes have been observed on pl5s of most type C and type D retroviruscs (28).…”

Section: Interspecies Reactivity O/pme V-induced Suppression O/lymphomentioning

confidence: 99%

Suppression of human lymphocyte mitogen response by retroviruses of type D

Denner¹,

Indzhiia²,

Wunderlich³

et al. 1985

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In contrast to the human cell line derived type D retrovirus PMFV, the Mason-Pfizer monkey virus (MPMV) does not suppress the mitogen response of normal human lymphocytes. Both viruses have been propagated on the same cell lines and purified by the same methods. MPMV did not contain a factor able to abolish PMFV-induced suppression of the mitogen response. Neither could MPMV suppress the mitogen response of lymphocytes from rhesus monkeys or baboons. PMFV however inhibited their reactivity.

Further evidence from tryptic peptide analysis for heterogeneity among type D retroviruses

Uckert¹,

Hertling²,

Denner³

1984

Tryptic peptide analyses were performed on the major internal structural proteins of type D retroviruses isolated originally from subhuman primate species (MPMV, SMRV, LV) and from permanent human cell lines (HeLaV, HEp-2V, PMFV). The p25 peptide maps of MPMV, LV, PMFV and HEp-2V were very similar but showed a striking dissimilarity to the p25 map of HeLaV and the p35 map of SMRV. All type D viruses included in this study could be distinguished by peptide maps of at least two of their three low-molecular-weight proteins p10, p12 and p15. These studies further demonstrate the heterogeneity among type D retroviruses.