Serological Epidemiology of Reovirus Infection (1 Report)

Fukumi, Hideo; Takeuchi, Yasue; Ishida, Masatoshi; Saito, Hideyuki

doi:10.7883/yoken1952.22.13

Cited by 5 publications

(5 citation statements)

References 5 publications

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“…Similar trends in reovirus infections containing multiple serotypes have been previously reported in healthy dogs [ 28 , 29 , 30 ], cattle [ 31 ], swine [ 32 ] and humans [ 9 , 33 , 34 ]. Infections with multiple MRVs represent the basis for the genesis of new reassortant strains in permissive hosts, and insectivorous bats may play an important role in this genesis.…”

Section: Discussionsupporting

confidence: 80%

Detection and Characterization of a Novel Reassortant Mammalian Orthoreovirus in Bats in Europe

Lelli

Moreno

Steyer

et al. 2015

Viruses

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A renewed interest in mammalian orthoreoviruses (MRVs) has emerged since new viruses related to bat MRV type 3, detected in Europe, were identified in humans and pigs with gastroenteritis. This study reports the isolation and characterization of a novel reassortant MRV from the lesser horseshoe bat (Rhinolophus hipposideros). The isolate, here designated BatMRV1-IT2011, was first identified by electron microscopy and confirmed using PCR and virus-neutralization tests. The full genome sequence was obtained by next-generation sequencing. Molecular and antigenic characterizations revealed that BatMRV1-IT2011 belonged to serotype 1, which had not previously been identified in bats. Phylogenetic and recombination detection program analyses suggested that BatMRV1-IT2011 was a reassortant strain containing an S1 genome segment similar to those of MRV T1/bovine/Maryland/Clone23/59 and C/bovine/Indiana/MRV00304/2014, while other segments were more similar to MRVs of different hosts, origins and serotypes. The presence of neutralizing antibodies against MRVs has also been investigated in animals (dogs, pigs, bovines and horses). Preliminary results suggested that MRVs are widespread in animals and that infections containing multiple serotypes, including MRVs of serotype 1 with an S1 gene similar to BatMRV1-IT2011, are common. This paper extends the current knowledge of MRVs and stresses the importance to continue and improve MRV surveillance in bats and other mammals through the development and standardization of specific diagnostic tools.

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Section: Discussionsupporting

confidence: 80%

Detection and Characterization of a Novel Reassortant Mammalian Orthoreovirus in Bats in Europe

Lelli

Moreno

Steyer

et al. 2015

Viruses

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“…As reovirus is commonly isolated from contaminated water sources [15, 18, 30], dogs housed outdoor are prone to exposure to reovirus. This result coincides with a report where a higher percentage of stray dogs were found to be reovirus seropositive [6]. …”

supporting

confidence: 92%

“…Hence, reovirus infection of more than one serotype can still occur depending on exposure. Similar trends of reovirus infection of multiple serotypes have been previously reported in healthy dogs [6, 17, 19], cattle [24], swine [7] and humans [20, 21, 31]. This finding has provided important information, suggesting that neutralizing antibodies against reovirus T1L and T2A will not affect the usage of reovirus T3D as therapy in canine cancers.…”

supporting

confidence: 79%

“…However, unlike in humans, seroepidemiological data of reovirus in healthy dogs are limited. Reports have indicated that 14–63% of sampled dog populations have an elevated reovirus neutralizing antibody titer [5, 6, 17, 19]. Even though isolation of various serotypes of reovirus from dogs and cats has been reported, it is usually incidental [2, 4, 9, 11, 13, 14, 16, 27].…”

mentioning

confidence: 99%

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Seroepidemiology of Reovirus in Healthy Dogs in Six Prefectures in Japan

Hwang

Mochizuki

Maeda

et al. 2014

The Journal of Veterinary Medical Science

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Reovirus infection is common in mammals. However, seroepidemiological data of reovirus neutralizing antibodies are limited in dogs. In this study, sera of 65 healthy dogs from six prefectures across Japan were tested for neutralizing antibodies against reovirus serotype 1 strain Lang (T1L), serotype 2 strain Amy (T2A) and serotype 3 strain Dearing (T3D) using plaque reduction neutralization test (PRNT). Seropositivity against reovirus T1L, T2A and T3D was 53.85%, 33.85% and 46.15%, respectively. Distribution of reovirus seropositive samples displayed no distinguishable geographical pattern. However, reovirus seropositivity increased with age and in dogs housed outdoor. Co-infection of multiple reovirus serotypes in dogs was also detected. These data will provide valuable insights towards the usage of reovirus in oncolytic virotherapy in canine cancers.

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“…Unfortunately, unlike in the human population, limited data is available regarding reovirus infection and antibody production in dogs [48]–[50]. Therefore, prediction of the effects of pre-existing neutralizing antibodies in reovirus therapy in dogs is difficult.…”

Section: Discussionmentioning

confidence: 99%

Oncolytic Reovirus in Canine Mast Cell Tumor

et al. 2013

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The usage of reovirus has reached phase II and III clinical trials in human cancers. However, this is the first study to report the oncolytic effects of reovirus in veterinary oncology, focusing on canine mast cell tumor (MCT), the most common cutaneous tumor in dogs. As human and canine cancers share many similarities, we hypothesized that the oncolytic effects of reovirus can be exploited in canine cancers. The objective of this study was to determine the oncolytic effects of reovirus in canine MCT in vitro, in vivo and ex vivo. We demonstrated that MCT cell lines were highly susceptible to reovirus as indicated by marked cell death, high production of progeny virus and virus replication. Reovirus induced apoptosis in the canine MCT cell lines with no correlation to their Ras activation status. In vivo studies were conducted using unilateral and bilateral subcutaneous MCT xenograft models with a single intratumoral reovirus treatment and apparent reduction of tumor mass was exhibited. Furthermore, cell death was induced by reovirus in primary canine MCT samples in vitro. However, canine and murine bone marrow-derived mast cells (BMCMC) were also susceptible to reovirus. The combination of these results supports the potential value of reovirus as a therapy in canine MCT but warrants further investigation on the determinants of reovirus susceptibility.

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Serological Epidemiology of Reovirus Infection (1 Report)

Cited by 5 publications

References 5 publications

Detection and Characterization of a Novel Reassortant Mammalian Orthoreovirus in Bats in Europe

Detection and Characterization of a Novel Reassortant Mammalian Orthoreovirus in Bats in Europe

Seroepidemiology of Reovirus in Healthy Dogs in Six Prefectures in Japan

Oncolytic Reovirus in Canine Mast Cell Tumor

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